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2006

Conference Publication

The impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.

Bialasiewicz S., Whiley D. and Sloots, Theodorus P. (2006). The impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.. Annual Health and Medical Research Conference of Queensland, Brisbane, Australia., 2006.

The impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR.

2006

Journal Article

Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays

Whiley, David M. and Sloots, Theo P. (2006). Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays. Journal of Clinical Virology, 35 (1), 81-83. doi: 10.1016/j.jcv.2005.05.002

Sequence variation can affect the performance of minor groove binder TaqMan probes in viral diagnostic assays

2006

Journal Article

Nucleic acid amplification testing for Neisseria gonorrhoeae: An ongoing challenge

Whiley, David M., Tapsall, John W. and Sloots, Theo P. (2006). Nucleic acid amplification testing for Neisseria gonorrhoeae: An ongoing challenge. Journal of Molecular Diagnostics, 8 (1), 3-15. doi: 10.2353/jmoldx.2006.050045

Nucleic acid amplification testing for Neisseria gonorrhoeae: An ongoing challenge

2006

Journal Article

Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target

Whiley, David M., Anderson, Trevor P., Barratt, Kevin, Beaman, Miles H., Buda, Philip J., Carter, Martine, Freeman, Kevin, Hallsworth, Peter, Limnios, E. Athena, Lum, Gary, Merien, Fabrice, Vernel-Pauillac, Frédérique, Tapsall, John W., Witt, Michael J., Nissen, Michael D. and Sloots, Theo P. (2006). Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target. Pathology, 38 (5), 445-448. doi: 10.1080/00313020600928253

Evidence that the gonococcal porA pseudogene is present in a broad range of Neisseria gonorrhoeae strains; suitability as a diagnostic target

2006

Book Chapter

Clinical Virology

Whiley, D. M. and Sloots, T. P. (2006). Clinical Virology. Real-Time PCR. (pp. 231-276) edited by M. Tevfik Dorak. UK: Taylor & Francis.

Clinical Virology

2005

Journal Article

A 5 '-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1

Whiley, David M. and Sloots, Theo P. (2005). A 5 '-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1. Diagnostic Microbiology and Infectious Disease, 53 (4), 335-337. doi: 10.1016/j.diagmicrobio.2005.08.002

A 5 '-nuclease real-time reverse transcriptase-polymerase chain reaction assay for the detection of a broad range of influenza A subtypes, including H5N1

2005

Journal Article

Sequence variation in primer targets affects the accuracy of viral quantitative PCR

Whiley, David M. and Sloots, Theo P. (2005). Sequence variation in primer targets affects the accuracy of viral quantitative PCR. Journal of Clinical Virology, 34 (2), 104-107. doi: 10.1016/j.jcv.2005.02.010

Sequence variation in primer targets affects the accuracy of viral quantitative PCR

2005

Journal Article

A cluster of culture positive gonococcal infections but with false negative cppB gene based PCR

Lum, G., Freeman, K., Nguyen, N. L., Limnios, E. A., Tabrizi, S. M., Carter, I., Chambers, I. W., Sloots, T. P., Whiley, D. M., Garland, S. M. and Tapsall, J. (2005). A cluster of culture positive gonococcal infections but with false negative cppB gene based PCR. Sexually Transmitted Infections, 81 (5), 400-402. doi: 10.1136/sti.2004.013805

A cluster of culture positive gonococcal infections but with false negative cppB gene based PCR

2005

Journal Article

A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens

Whiley, David M., Buda, Philip P., Freeman, Kevin, Prattle, Neville I., Bates, John and Sloots, Theo P. (2005). A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens. Diagnostic Microbiology and Infectious Disease, 52 (1), 1-5. doi: 10.1016/j.diagmicrobio.2004.12.011

A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens

2005

Journal Article

Cryptic-plasmid-free gonococci may contribute to failure of cppB gene-based assays to confirm results of BD ProbeTEC PCR for identification of Neisseria gonorrhoeae

Tapsall, J. W., Limnios, E. A., Nguyen, N. L., Carter, I., Lum, G., Freeman, K., Tabrizi, S. N., Garland, S. M., Whiley, D. M., Sloots, T. P. and Chambers, I. W. (2005). Cryptic-plasmid-free gonococci may contribute to failure of cppB gene-based assays to confirm results of BD ProbeTEC PCR for identification of Neisseria gonorrhoeae. Journal of Clinical Microbiology, 43 (4), 2036-2037. doi: 10.1128/JCM.43.4.2036-2037.2005

Cryptic-plasmid-free gonococci may contribute to failure of cppB gene-based assays to confirm results of BD ProbeTEC PCR for identification of Neisseria gonorrhoeae

2005

Journal Article

Melting curve analysis using hybridisation probes: limitations in microbial molecular diagnostics

Whiley, David M. and Sloots, Theo P. (2005). Melting curve analysis using hybridisation probes: limitations in microbial molecular diagnostics. Pathology, 37 (3), 254-256. doi: 10.1080/00313020500099270

Melting curve analysis using hybridisation probes: limitations in microbial molecular diagnostics

2005

Journal Article

Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies

Whiley, David M. and Sloots, Theo P. (2005). Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies. Pathology, 37 (5), 364-370. doi: 10.1080/00313020500254552

Comparison of three in-house multiplex PCR assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis using real-time and conventional detection methodologies

2004

Journal Article

A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene

Whiley, D. M., Buda, P. J., Bayliss, J., Cover, L., Bates, J. and Sloots, T. P. (2004). A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene. European Journal of Clinical Microbiology & Infectious Diseases, 23 (9), 705-710. doi: 10.1007/s10096-004-1170-0

A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene

2004

Journal Article

Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction

Whiley, David M., Mackay, Ian M., Syrmis, Melanie W., Witt, Michael J. and Sloots, Theo P. (2004). Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction. Journal of Clinical Virology, 30 (1), 32-38. doi: 10.1016/j.jcv.2003.08.003

Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction

2004

Journal Article

Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria

Whiley, David M., LeCornec, Genevera M., Baddeley, Annette, Savill, John, Syrmis, Melanie W., Mackay, Ian M., Siebert, David J., Burns, Dominic, Nissen, Michael and Sloots, Theo P. (2004). Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria. Diagnostic Microbiology And Infectious Disease, 49 (1), 25-29. doi: 10.1016/j.diagmicrobio.2003.10.014

Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria

2004

Journal Article

A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples

Syrmis, Melanie W., Whiley, David M., Thomas, Marion, Mackay, Ian M., Williamson, Jeanette, Siebert, David J., Nissen, Michael D. and Sloots, Theo P. (2004). A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples. Journal of Molecular Diagnostics, 6 (2), 125-131. doi: 10.1016/S1525-1578(10)60500-4

A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples

2004

Journal Article

Simultaneous detection and differentiation of human Polyomaviruses JC and BK by a rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population

Whiley, David M., Arden, Katherine E., Mackay, Ian M., Syrmis, Melanie W. and Sloots, Theo P. (2004). Simultaneous detection and differentiation of human Polyomaviruses JC and BK by a rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population. Journal of Medical Virology, 72 (3), 467-472. doi: 10.1002/jmv.20005

Simultaneous detection and differentiation of human Polyomaviruses JC and BK by a rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population

2003

Journal Article

Preliminary comparison of three lightcycler pcr assays for the detection of herpes simplex virus in swab specimens

Whiley, D. M., Syrmis, M. W., Mackay, I. M. and Sloots, T. P. (2003). Preliminary comparison of three lightcycler pcr assays for the detection of herpes simplex virus in swab specimens. European Journal of Clinical Microbiology & Infectious Diseases, 22 (12), 764-767. doi: 10.1007/s10096-003-1031-2

Preliminary comparison of three lightcycler pcr assays for the detection of herpes simplex virus in swab specimens

2003

Journal Article

Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA

Mackay, Ian M., Gardam, Tim, Arden, Katherine E., McHardy, Suzi, Whiley, David M., Crisante, Erin and Sloots, Theo P. (2003). Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA. Journal of Clinical Virology, 28 (3), 291-302. doi: 10.1016/S1386-6532(03)00072-6

Co-detection and discrimination of six human herpesviruses by multiplex PCR-ELAHA

2003

Journal Article

Detection of Neisseria meningitidis by LightCycler PCR

Whiley, David M., Crisante, Michelle E., Syrmis, Melanie W., Mackay, Ian M. and Sloots, Theo P. (2003). Detection of Neisseria meningitidis by LightCycler PCR. Pathology, 35 (4), 347-349. doi: 10.1080/0031302031000152874

Detection of Neisseria meningitidis by LightCycler PCR