Sustaining neuronal communication through bulk endocytosis (2009-2011)

Neuronal communication relies on the release of neurotransmitter from synaptic vesicles. In order to sustain a high level of communication, synaptic vesicles are recycled locally. Neurons need to keep the number of synaptic vesicles relatively stable in order to cope with periods of intense activity. This project aims at exploring the fundamental role of bulk endocytosis in recycling synaptic vesicles. We will characterize the nature and formation of large invaginations from which synaptic vesicles emanate following sustained nerve stimulation. We will demonstrate that interfering with bulk endocytosis blocks neurons ability to maintain neurotransmitter release.