Advanced optical tweezers technologies for biophysical measurements (2010-2014)

We will use new optical tweezers technologies and methods to measure forces, torques, and mechanical properties in biophysical systems, including single molecules, molecular motors, and cells. These measurements will make major contributions to the understanding of the molecular mechanisms by which cells produce or respond to mechanical forces and other important biophysical problems. Using recently developed and new methods, including the use of rotating probe particles and optically-driven micromachines, we will be able to meake measurements not previously possible. Outcomes will include both direct measurements and the development of new instruments and methods.