Live molecular imaging using super resolution microscopy, two photon and spinning disk confocal microscopy (2013-2014)
Abstract
A new era of molecular live imaging has begun which allows rapid 3-D visualisation of biological processes such as brain wiring in vivo down to the tracking of a single molecule. This relies on recent advances in imaging including multiphoton, spinning-disk and particularly super-resolution microscopy. The goal of this application is to establish the first multi-instrument super-resolution system in Queensland, allowing a team of multi-institutional cross-disciplinary researchers to use these emerging technologies to image single molecules within the cellular environment in living cells. Such insight is key to understanding basical biological interactions that govern brain wiring, communication between cells, and cell-cell adhesion.
