Development of cryopreservation for high value provenance collections of recalcitrant plant species used in post-mining restoration (2009-2011)
Abstract
This project will investigate the major factors that determine the ability of various plant species to survive cryogenic storage, with a focus on recalcitrant and/or endangered species of relevance to post-mining rehabilitation programs. The project will focus on defining membrane model systems for representative plant species, designing experimental and modelling predictions to provide guidelines for cryopreservation protocols for in vitro culture collections of valuable and irreplaceable genotypes and applying model systems to a wider range of species to assess the robustness of these models in predicting optimum cryogenic conditions.