High resolution single particle analysis of biological macromolecules (2004-2006)
Abstract
One of the great challenges of cell biology is to increase the rate of atomic resolution structure determination particularly of membrane proteins and macromolecular assemblies. The current rate-limiting step is high quality crystal production. Our goal is to prove that protein structures can be determined to atomic resolution by single-particle analysis. 3D structures will be produced by computationally aligning high-resolution electron microscope images of individual randomly oriented molecules. The importance of this project is highlighted by the fact over
