
Overview
Background
A life-long fascination in sciences provided me with the inspiration to graduate in exercise physiology (University of Sherbrooke, Canada, 2004), complete a PhD in physiology/biophysics (University of Sherbrooke, 2009) and continue in my current role as a postdoctoral researcher at the School of Biomedical Sciences (SBMS) of The University of Queensland. I am a physiologist first and foremost with a particular interest in understanding how skeletal muscle cell normally functions so as to try and elucidate what changes or factors contribute to various forms of muscle weakness with ageing, inactivity or various chronic diseases.
During my previous postdoctoral appointment at La Trobe University (Melbourne, 2010-2017), I have gained considerable experience using the "mechanically skinned muscle fibre" technique in animal muscle. Importantly, I have developed this technique for the first time in human muscle which allows the exciting opportunity to investigate cellular mechanisms of muscle weakness in different clinical population. This is vitally important since most of our existing knowledge on muscle function comes from studies on muscles obtained from animal models. This technical breakthrough has been recognized by editorials of different leading scientific journals in the field of Physiology. I’m now a world recognized expert of this technique which has immense potential for examining any number of physiological questions and even allows for biochemical analyses of any protein of interest in the same cell.
Availability
- Dr Cedric Lamboley is:
- Not available for supervision
Fields of research
Research interests
-
Skeletal muscle weakness with ageing and inactivity
This research interest is central to any understanding of the mechanism(s) underlying abnormalities in protein expression and oxidation-induced dysfunction responsible for marked muscle weakness and fatigability occurring during ageing or inactivity. The aims of this study is to identify the specific mechanism and target protein responsible for the loss of muscle force in these populations. This is the first study to comprehensively investigate the effects of ageing and inactivity on the physiological and biochemical changes in human skeletal muscle at the single muscle cell level. Protecting muscle from exacerbated levels of oxidative stress represents a critical therapeutic approach to improve muscle function and quality of life of aged and inactive persons until a cure is developed. The identification in these populations of the precise molecular site and mechanisms involved in oxidative damage would be highly significant and make it realistic to design drugs to bind and protect this molecular site, and thereby aid muscle performance in disease states.
-
Ryanodine receptor mutations and skeletal muscle weakness
Ryanodine receptors (RyR) are the Ca2+ release channels of skeletal muscle fibres that regulate muscle contraction. There are many known mutations in the RyR that can affect the function of this channel. In the event of mutated RyRs the outcome for an individual can be no obvious effect on muscle strength, or mild, through to very severe and life-shortening myopathy. As the severity of the mutation increases, so does the weakness of the muscle. The aim of this project is to apply the latest techniques established in our lab to assess how calcium moves in muscle with these mutations to find targets to improve muscle strength in this disease.
Research impacts
My current postdoctoral research project within Brad Launikonis’ lab utilizes my unique abilities to perform comprehensive physiological examinations (skinned muscle fibre technique coupled with confocal microscopy) on single segments of individual fresh muscle cells, obtained from animal models or muscle biopsies of human subjects. No other research group other than ours is able to produce such results to date. This new and exciting development will open up enormous potential applications in physiotherapy, human physiology, understanding muscle fatigue and adaptability, as well as uncovering basic muscle defects in disease in humans.
I have made several important contributions to the field of human muscle physiology. Most notably, my research helped to debunk the commonly held misconception that the deficit in Ca2+ release from the sarcoplasmic reticulum (SR) observed in aged skeletal muscle was induced by a reduction in the number of voltage sensors coupled with the adjacent Ca2+ release channels (RyRs) (excitation-contraction uncoupling). Instead, my recent results demonstrated for the first time that there is an increased leakage of Ca2+ out of the SR through the RyRs in type I muscle fibres in aged humans as a result of the oxidative modification of the RyRs. This Ca2+ leakage is probably the primary cause of the decreased available SR Ca2+ content seen in such fibres and is a major contributing factor involved in muscle atrophy and weakness with ageing (Lamboley et al., J. Physiol. 2015, 2016). Such SR Ca2+ leakage and depletion in human fibres may arise from a self-reinforcing cycle in which Ca2+ leakage through the RyRs leads to increased reactive oxygen species production by the mitochondria, which in turn further exacerbates RyR leakage.
Works
Search Professor Cedric Lamboley’s works on UQ eSpace
2023
Journal Article
Ryanodine receptor activity and store‐operated Ca <sup>2+</sup> entry: critical regulators of Ca <sup>2+</sup> content and function in skeletal muscle
Pearce, Luke, Meizoso‐Huesca, Aldo, Seng, Crystal, Lamboley, Cedric R., Singh, Daniel P. and Launikonis, Bradley S. (2023). Ryanodine receptor activity and store‐operated Ca 2+ entry: critical regulators of Ca 2+ content and function in skeletal muscle. The Journal of Physiology, 601 (19), 4183-4202. doi: 10.1113/jp279512
2023
Journal Article
A bivalent remipede toxin promotes calcium release via ryanodine receptor activation
Maxwell, Michael J., Thekkedam, Chris, Lamboley, Cedric, Chin, Yanni K.-Y., Crawford, Theo, Smith, Jennifer J., Liu, Junyu, Jia, Xinying, Vetter, Irina, Laver, Derek R., Launikonis, Bradley S., Dulhunty, Angela, Undheim, Eivind A. B. and Mobli, Mehdi (2023). A bivalent remipede toxin promotes calcium release via ryanodine receptor activation. Nature Communications, 14 (1) 1036, 1-13. doi: 10.1038/s41467-023-36579-w
2023
Journal Article
Evolutionary isolation of ryanodine receptor isoform 1 for muscle-based thermogenesis in mammals
Singh, Daniel P., Pearce, Luke, Choi, Rocky H., Meizoso-Huesca, Aldo, Wette, Stefan G., Scott, John W., Lamboley, Cedric R., Murphy, Robyn M. and Launikonis, Bradley S. (2023). Evolutionary isolation of ryanodine receptor isoform 1 for muscle-based thermogenesis in mammals. Proceedings of the National Academy of Sciences, 120 (4) e2117503120, 1-12. doi: 10.1073/pnas.2117503120
2022
Journal Article
Tiny changes in cytoplasmic [Ca<sup>2+</sup>] cause large changes in mitochondrial Ca<sup>2+</sup>: what are the triggers and functional implications?
Seng, Crystal, Pearce, Luke, Meizoso-Huesca, Aldo, Singh, Daniel P., Murphy, Robyn M., Lamboley, Cedric and Launikonis, Bradley S. (2022). Tiny changes in cytoplasmic [Ca2+] cause large changes in mitochondrial Ca2+: what are the triggers and functional implications?. American Journal of Physiology-Cell Physiology, 323 (4), C1285-C1289. doi: 10.1152/ajpcell.00092.2022
2021
Journal Article
Ryanodine receptor leak triggers fiber Ca2+ redistribution to preserve force and elevate basal metabolism in skeletal muscle
Lamboley, Cedric R., Pearce, Luke, Seng, Crystal, Meizoso-Huesca, Aldo, Singh, Daniel P., Frankish, Barnaby P., Kaura, Vikas, Lo, Harriet P., Ferguson, Charles, Allen, Paul D., Hopkins, Philip M., Parton, Robert G., Murphy, Robyn M., van der Poel, Chris, Barclay, Christopher J. and Launikonis, Bradley S. (2021). Ryanodine receptor leak triggers fiber Ca2+ redistribution to preserve force and elevate basal metabolism in skeletal muscle. Science Advances, 7 (44) eabi7166, 1-15. doi: 10.1126/sciadv.abi7166
2020
Journal Article
Effects of high-intensity intermittent exercise on the contractile properties of human type I and type II skeletal muscle fibers
Lamboley, Cedric R., Rouffet, David M., Dutka, Travis L., McKenna, Michael J. and Lamb, Graham D. (2020). Effects of high-intensity intermittent exercise on the contractile properties of human type I and type II skeletal muscle fibers. Journal of Applied Physiology, 128 (5), 1207-1216. doi: 10.1152/japplphysiol.00014.2020
2020
Conference Publication
Total Calcium Content of Sarcoplasmic Reticulum and Mitochondria in Ryanodine Receptor Variant Muscle
Lamboley, Cedric R., Pearce, Luke and Launikonis, Bradley S. (2020). Total Calcium Content of Sarcoplasmic Reticulum and Mitochondria in Ryanodine Receptor Variant Muscle. In: 64th Annual Meeting of the Biophysical Society, San Diego, CA United States, (98A-99A). 15-19 February 2020.
2019
Journal Article
Effects of S-glutathionylation on the passive force-length relationship in skeletal muscle fibres of rats and humans
Watanabe, Daiki, Lamboley, Cedric R. and Lamb, Graham D. (2019). Effects of S-glutathionylation on the passive force-length relationship in skeletal muscle fibres of rats and humans. Journal of Muscle Research and Cell Motility, 41 (2-3), 239-250. doi: 10.1007/s10974-019-09563-5
2019
Journal Article
Skeletal muscle fibre swelling contributes to force depression in rats and humans: a mechanically-skinned fibre study
Watanabe, Daiki, Dutka, Travis L., Lamboley, Cedric R. and Lamb, Graham D. (2019). Skeletal muscle fibre swelling contributes to force depression in rats and humans: a mechanically-skinned fibre study. Journal of Muscle Research and Cell Motility, 40 (3-4), 343-351. doi: 10.1007/s10974-019-09521-1
2018
Journal Article
Effect of androgen deprivation therapy on the contractile properties of type I and type II skeletal muscle fibres in men with non-metastatic prostate cancer
Lamboley, Cedric R., Xu, Hongyang, Dutka, Travis L., Hanson, Erik D., Hayes, Alan, Violet, John A., Murphy, Robyn M. and Lamb, Graham D. (2018). Effect of androgen deprivation therapy on the contractile properties of type I and type II skeletal muscle fibres in men with non-metastatic prostate cancer. Clinical and Experimental Pharmacology and Physiology, 45 (2), 146-154. doi: 10.1111/1440-1681.12873
2017
Journal Article
S-nitrosylation and S-glutathionylation of Cys134 on troponin I have opposing competitive actions on Ca2+ sensitivity in rat fast-twitch muscle fibers
Dutka, T. L., Mollica, J. P., Lamboley, C. R., Weerakkody, V. C., Greening, D. W., Posterino, G. S., Murphy, R. M. and Lamb, G. D. (2017). S-nitrosylation and S-glutathionylation of Cys134 on troponin I have opposing competitive actions on Ca2+ sensitivity in rat fast-twitch muscle fibers. American Journal of Physiology: Cell Physiology, 312 (3), C316-C327. doi: 10.1152/ajpcell.00334.2016
2016
Journal Article
Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca2+ properties and contractility in human type i and type II skeletal muscle fibers
Lamboley, C. R., Wyckelsma, V. L., Perry, B. D., McKenna, M. J. and Lamb, G. D. (2016). Effect of 23-day muscle disuse on sarcoplasmic reticulum Ca2+ properties and contractility in human type i and type II skeletal muscle fibers. Journal of Applied Physiology, 121 (2), 483-492. doi: 10.1152/japplphysiol.00337.2016
2016
Journal Article
Cell specific differences in the protein abundances of GAPDH and Na+,K+-ATPase in skeletal muscle from aged individuals
Wyckelsma, Victoria L., McKenna, Michael J., Levinger, Itamar, Petersen, Aaron C., Lamboley, Cedric R. and Murphy, Robyn M. (2016). Cell specific differences in the protein abundances of GAPDH and Na+,K+-ATPase in skeletal muscle from aged individuals. Experimental Gerontology, 75, 8-15. doi: 10.1016/j.exger.2015.12.010
2016
Journal Article
Ca2+ leakage out of the sarcoplasmic reticulum is increased in type I skeletal muscle fibres in aged humans
Lamboley, C. R., Wyckelsma, V. L., Mckenna, M. J., Murphy, R. M. and Lamb, G. D. (2016). Ca2+ leakage out of the sarcoplasmic reticulum is increased in type I skeletal muscle fibres in aged humans. Journal of Physiology, 594 (2), 469-481. doi: 10.1113/JP271382
2015
Journal Article
Contractile properties and sarcoplasmic reticulum calcium content in type I and type II skeletal muscle fibres in active aged humans
Lamboley, C. R., Wyckelsma, V. L., Dutka, T. L., McKenna, M. J., Murphy, R. M. and Lamb, G. D. (2015). Contractile properties and sarcoplasmic reticulum calcium content in type I and type II skeletal muscle fibres in active aged humans. Journal of Physiology, 593 (11), 2499-2514. doi: 10.1113/JP270179
2015
Journal Article
Single-fiber expression and fiber-specific adaptability to short-term intense exercise training of Na+-K+-ATPaseα- and α-isoforms in human skeletal muscle
Wyckelsma, V. L., McKenna, M. J., Serpiello, F. R., Lamboley, C. R., Aughey, R. J., Stepto, N. K., Bishop, D. J. and Murphy, R. M. (2015). Single-fiber expression and fiber-specific adaptability to short-term intense exercise training of Na+-K+-ATPaseα- and α-isoforms in human skeletal muscle. Journal of Applied Physiology, 118 (6), 699-706. doi: 10.1152/japplphysiol.00419.2014
2015
Journal Article
New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin
Lamboley, Cédric R. H., Guena, Sandrine A. Kake, Touré, Fatou, Hébert, Camille, Yaddaden, Louiza, Nadeau, Stephanie, Bouchard, Patrice, Wei-LaPierre, Lan, Lainé, Jean, Rousseau, Eric C., Frenette, Jérôme, Protasi, Feliciano, Dirksen, Robert T. and Pape, Paul C. (2015). New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin. Journal of General Physiology, 145 (2), 127-153. doi: 10.1085/jgp.201411250
2014
Journal Article
Acute effects of taurine on sarcoplasmic reticulum Ca2+ accumulation and contractility in human type i and type II skeletal muscle fibers
Dutka, T. L., Lamboley, C. R., Murphy, R. M. and Lamb, G. D. (2014). Acute effects of taurine on sarcoplasmic reticulum Ca2+ accumulation and contractility in human type i and type II skeletal muscle fibers. Journal of Applied Physiology, 117 (7), 797-805. doi: 10.1152/japplphysiol.00494.2014
2014
Journal Article
Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle
Lamboley, C. R., Murphy, R. M., Mckenna, M. J. and Lamb, G. D. (2014). Sarcoplasmic reticulum Ca2+ uptake and leak properties, and SERCA isoform expression, in type I and type II fibres of human skeletal muscle. Journal of Physiology, 592 (6), 1381-1395. doi: 10.1113/jphysiol.2013.269373
2014
Conference Publication
New Method for Determining the Total Calcium Content of Tissue Applied to Whole Skeletal Muscles from Mice with and Without Calsequestrin Knocked Out
Kake-Guena, Sandrine A., Lamboley, Cedric R. H., Bouchard, Patrice, Frenette, Jerome, Rousseau, Eric, Dirksen, Robert T., Protasi, Feliciano and Pape, Paul C. (2014). New Method for Determining the Total Calcium Content of Tissue Applied to Whole Skeletal Muscles from Mice with and Without Calsequestrin Knocked Out. 58th Annual Meeting of the Biophysical-Society, San Francisco Ca, Feb 15-19, 2014. CAMBRIDGE: CELL PRESS. doi: 10.1016/j.bpj.2013.11.4033
Supervision
Availability
- Dr Cedric Lamboley is:
- Not available for supervision
Supervision history
Completed supervision
-
2024
Master Philosophy
RyR1 Myopathy: A model of hypermetabolism
Associate Advisor
Other advisors: Dr David Simmons, Professor Bradley Launikonis
Media
Enquiries
For media enquiries about Dr Cedric Lamboley's areas of expertise, story ideas and help finding experts, contact our Media team: