Overview
Background
I am a reproductive biologist specialising in assisted reproductive technologies (ART), with a focus on IVF, ICSI, cloning, and embryo technologies for animal production and wildlife conservation. My work aims to improve our understanding of reproductive biology and develop innovative reproductive biotechnologies across livestock, equine, and wildlife species, with strong links to industry and international collaborators.
My work has delivered key advances in the field, including:
- First cloned horses in South America (2010) and Australia (2018)
- Development of efficient OPU–ICSI systems in horses
- First in vitro-produced kangaroo embryos (2025)
- Embryo production across species, including donkeys and mules (2022)
- First cloned zebra embryos worldwide (2020)
With international research experience in South America, the USA and Europe, my current work focuses on optimising cloning IVF and ICSI systems, identifying biomarkers of gamete and embryo quality and developing biobanking strategies for threatened species biology.
I welcome opportunities for HDR supervision, research collaboration, and industry partnerships in animal reproduction and conservation technologies.
Availability
- Dr Andres Gambini is:
- Available for supervision
- Media expert
Fields of research
Qualifications
- Graduate Diploma in Veterinary Medicine, Universidad Nacional de Río Cuarto
- Doctor of Philosophy of Animal Reproduction, Universidad de Buenos Aires
Research interests
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Gamete Preservation Technologies
Developing and optimizing innovative methods for the cryopreservation and in vitro storage of gametes to enhance reproductive success in livestock and wildlife. My research focuses on improving sperm, oocyte, and embryo viability post-thaw, with applications in conservation, assisted reproductive technologies, and genetic improvement. This work supports sustainable breeding programs, biodiversity preservation, and advancements in fertility treatments for endangered and domestic species.
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In Vitro Embryo Development for Animal Production and Conservation
Advancing in vitro embryo production to improve reproductive efficiency in livestock and support conservation efforts for endangered species. My research focuses on optimizing culture conditions, embryo viability, and developmental competence to enhance breeding outcomes. These technologies contribute to genetic improvement, sustainable animal production, and the preservation of biodiversity by enabling the propagation of valuable or at-risk genetic lines.
Research impacts
My research delivers impact in animal production and wildlife conservation by applying advanced reproductive technologies to real-world challenges.
In the livestock and equine industries, my work on IVF improves embryo production efficiency, supporting genetic gain, reproductive performance, and economic outcomes. These technologies are actively translated through collaborations with veterinarians and industry partners.
My research also builds capacity by training students and researchers, and by developing biobanking and reproductive platforms that can be applied across species.
Overall, my work connects science and application to support sustainable animal production and conservation outcomes.
Works
Search Professor Andres Gambini’s works on UQ eSpace
2021
Journal Article
Aggregation of Leopardus geoffroyi hybrid embryos with domestic cat tetraploid blastomeres
Duque Rodriguez, Matteo, Gambini, Andres, Ratner, Laura D., Sestelo, Adrian J., Briski, Olinda, Gutnisky, Cynthia, Rulli, Susana B., Fernandez Martin, Rafael, Cetica, Pablo and Salamone, Daniel F. (2021). Aggregation of Leopardus geoffroyi hybrid embryos with domestic cat tetraploid blastomeres. Reproduction, 161 (5), 539-548. doi: 10.1530/REP-20-0589
2020
Journal Article
Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern
Gambini, Andres, Duque Rodriguez, Matteo, Belen Rodriguez, Maria, Briski, Olinda, Flores Bragulat, Ana P., Demergassi, Natalia, Losinno, Luis and Salamone, Daniel F. (2020). Horse ooplasm supports in vitro preimplantation development of zebra ICSI and SCNT embryos without compromising YAP1 and SOX2 expression pattern. PLoS One, 15 (9) e0238948, e0238948. doi: 10.1371/journal.pone.0238948
2020
Journal Article
Developmentally programmed tankyrase activity upregulates beta-catenin and licenses progression of embryonic genome activation
Gambini, Andres, Stein, Paula, Savy, Virginia, Grow, Edward J., Papas, Brian N., Zhang, Yingpei, Kenan, Anna C., Padilla-Banks, Elizabeth, Cairns, Bradley R. and Williams, Carmen J. (2020). Developmentally programmed tankyrase activity upregulates beta-catenin and licenses progression of embryonic genome activation. Developmental Cell, 53 (5), 545-560. doi: 10.1016/j.devcel.2020.04.018
2020
Conference Publication
Ability of the zebra sperm to induce pig and horse oocyte activation and in vitro preimplantation embryo development after ICSI
Gambini, A., Rodriguez, M. B., Briski, O., Flores Bragulat, A., Demergasi, N., Losinno, L. and Salamone, D. F. (2020). Ability of the zebra sperm to induce pig and horse oocyte activation and in vitro preimplantation embryo development after ICSI. 10th International Symposium on Equine Embryo Transfer, Pisa, Italy, 2-5 July 2020. Philadelphia, PA USA: Elsevier. doi: 10.1016/j.jevs.2020.103052
2019
Book Chapter
The relevance of the donor cell for horse cloning
Gambini, Andres and Salamone, Daniel (2019). The relevance of the donor cell for horse cloning. Equine assisted reproduction. (pp. 77-89) edited by L. Losinno, R. Sanchez and T. Stout. Colombia: FAVE Editorial.
2019
Journal Article
Targeting epigenetic nuclear reprogramming in aggregated cloned equine embryos
Teixeira, Thiago V. Damasceno, Fry, Richard C., McKinnon, Angus, Fry, Kerri L., Kelly, Jennifer M., Verma, Paul J., Burden, Chelsie, Salamone, Daniel F. and Gambini, Andres (2019). Targeting epigenetic nuclear reprogramming in aggregated cloned equine embryos. Reproduction Fertility and Development, 31 (12), 1885-1893. doi: 10.1071/RD19239
2019
Journal Article
Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection
Belen Rodriguez, Maria, Gambini, Andres, Clerico, Gabriel, Eugenia Ynsaurralde-Rivolta, Amada, Briski, Olinda, Largel, Hernan, Sansinena, Marina and Salamone, Daniel F. (2019). Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection. Reproduction Fertility and Development, 31 (12), 1805-1811. doi: 10.1071/RD19248
2018
Journal Article
Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse
Miao, Yi-Liang, Gambini, Andres, Zhang, Yingpei, Padilla-Banks, Elizabeth, Jefferson, Wendy N., Bernhardt, Miranda L., Huang, Weichun, Li, Leping and Williams, Carmen J. (2018). Mediator complex component MED13 regulates zygotic genome activation and is required for postimplantation development in the mouse. Biology of Reproduction, 98 (4), 449-464. doi: 10.1093/biolre/ioy004
2018
Conference Publication
A journey through horse cloning
Gambini, Andres and Maserati, Marc (2018). A journey through horse cloning. Annual Conference of the International Embryo Technology Society (IETS), Bangkok, Thailand, 13-16 January 2018. Clayton, VIC Australia: CSIRO. doi: 10.1071/RD17374
2016
Journal Article
Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse
Gambini, Andres, De Stefano, Adrian, Jarazo, Javier, Buemo, Carla, Karlanian, Florencia and Felipe Salamone, Daniel (2016). Embryo aggregation does not improve the development of interspecies somatic cell nuclear transfer embryos in the horse. Theriogenology, 86 (4), 1081-1091. doi: 10.1016/j.theriogenology.2016.03.040
2016
Journal Article
LUTs of blastocyst nuclei cell for quantification
Gambini, Andrés and Williams, Carmen J. (2016). LUTs of blastocyst nuclei cell for quantification. Molecular Reproduction and Development, 83 (7), 575-575. doi: 10.1002/mrd.22677
2016
Journal Article
Embryo aggregation in pig improves cloning efficiency and embryo quality
Paola Buemo, Carla, Gambini, Andres, Natalia Moro, Lucia, Ines Hiriart, Maria, Fernandez-Martin, Rafael, Collas, Philippe and Felipe Salamone, Daniel (2016). Embryo aggregation in pig improves cloning efficiency and embryo quality. PLoS One, 11 (2) e0146390. doi: 10.1371/journal.pone.0146390
2016
Other Outputs
1. Methods for Artificial Oocyte Activation
Bernhardt, Miranda L., Williams, Carmen J., Gambini, Andres and Mehlmann, Lisa M. (2016). 1. Methods for Artificial Oocyte Activation.
2014
Journal Article
The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos
Gambini, Andres, De Stefano, Adrian, Jimena Bevacqua, Romina, Karlanian, Florencia and Felipe Salamone, Daniel (2014). The aggregation of four reconstructed zygotes is the limit to improve the developmental competence of cloned equine embryos. PLoS One, 9 (11) e110998. doi: 10.1371/journal.pone.0110998
2014
Journal Article
Effect of collection-maturation interval time and pregnancy status of donor mares on oocyte developmental competence in horse cloning
Andrés, G., Javier, J., Florencia, K., De Stéfano, A. and Salamone, D. F. (2014). Effect of collection-maturation interval time and pregnancy status of donor mares on oocyte developmental competence in horse cloning. Journal of Animal Science, 92 (2), 561-567. doi: 10.2527/jas.2013-7026
2014
Journal Article
New micromanipulation technologies in domestic species Nuevas tecnologías de micromanipulación en especies domésticas
Salamone, D., Bevacqua, R., Gambini, A., Canel, N., Hiriart, M. I., Moro, L., Buemo, C. and Gibbons, A. (2014). New micromanipulation technologies in domestic species Nuevas tecnologías de micromanipulación en especies domésticas. Taurus, 16 (62), 66-68.
2013
Conference Publication
HORSE EMBRYO BIOPSY: EFFECT ON PREGNANCY RATES AND SUCCESSFUL SEX DETERMINATION DEPENDING ON THE SIZE OF THE EMBRYO
Jarazo, J., Gambini, A., Muredas, L., Fernandez-Martin, R. and Salamone, D. (2013). HORSE EMBRYO BIOPSY: EFFECT ON PREGNANCY RATES AND SUCCESSFUL SEX DETERMINATION DEPENDING ON THE SIZE OF THE EMBRYO. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv25n1Ab151
2013
Conference Publication
EFFECT OF CULTURE AT LOW OR ATMOSPHERIC OXYGEN TENSION IN SOMATIC DONOR CELLS FOR HORSE NUCLEAR TRANSFER
Gambini, A., Jarazo, J., De Stefano, A., Karlaninan, F. and Salamone, D. (2013). EFFECT OF CULTURE AT LOW OR ATMOSPHERIC OXYGEN TENSION IN SOMATIC DONOR CELLS FOR HORSE NUCLEAR TRANSFER. COLLINGWOOD: CSIRO PUBLISHING. doi: 10.1071/RDv25n1Ab35
2012
Conference Publication
Outgrowths and capsule derived from aggregated cloned equine embryos
Gambini, A., Jarazo, J., De Stefano, A., Karlanian, F. and Salamone, D. (2012). Outgrowths and capsule derived from aggregated cloned equine embryos. 17th International Congress on Animal Reproduction (ICAR), Vancouver Canada, Jul 29-Aug 02, 2012. HOBOKEN: WILEY-BLACKWELL.
2012
Journal Article
Equine cloning: in vitro and in vivo development of aggregated embryos
Gambini, Andres, Jarazo, Javier, Olivera, Ramiro and Salamone, Daniel F. (2012). Equine cloning: in vitro and in vivo development of aggregated embryos. Biology of Reproduction, 87 (1) 15. doi: 10.1095/biolreprod.112.098855
Supervision
Availability
- Dr Andres Gambini is:
- Available for supervision
Looking for a supervisor? Read our advice on how to choose a supervisor.
Available projects
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In vitro embryo development and gamete conservation for Animal Production and Conservation
Supervision history
Current supervision
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Doctor Philosophy
In-Vitro Approaches to Shield the Oocyte Activation Program from Heat-Induced Damage in Bovine Oocytes
Principal Advisor
Other advisors: Dr Vitor Mercadante
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Master Philosophy
Novel Techniques for Donkey and Horse Sperm and Somatic Cell Preservation
Principal Advisor
Other advisors: Associate Professor Albert Sole Guitart, Dr Taylor Pini
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Master Philosophy
Enhancing equid welfare in equine-assisted services. A survey of practitioner perspectives and practices in Australia.
Principal Advisor
Other advisors: Associate Professor Carlie Driscoll, Dr Suresh Krishnasamy
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Doctor Philosophy
Novel methods for improving in vitro production of bovine embryos
Principal Advisor
Other advisors: Associate Professor Steve Johnston
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Doctor Philosophy
Optimizing Equine OPU-ICSI Outcomes Through Enhanced In Vitro Maturation, Embryo Culture, and Sperm Selection Strategies
Principal Advisor
Other advisors: Associate Professor Gry Boe-Hansen
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Doctor Philosophy
Novel strategies for marsupial genetic preservation and in vitro embryo production
Principal Advisor
Other advisors: Associate Professor Steve Johnston
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Doctor Philosophy
Rescue, Rehabilitation and Stress Biology of Koala (Phascolarctos cinereus) Joeys
Associate Advisor
Other advisors: Dr Viviana Gonzalez Astudillo
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Doctor Philosophy
The investigation of the use of odour-detection dogs for non-invasive pregnancy diagnosis in cattle
Associate Advisor
Other advisors: Dr Kieren McCosker, Associate Professor Gry Boe-Hansen
Media
Enquiries
Contact Dr Andres Gambini directly for media enquiries about:
- animal conservation
- animal reproduction
- cattle
- cell culture
- cloning
- donkey
- egg
- embryo
- frozeen
- genetics
- horse reproduction
- ICSI
- IVF
- oocyte
- SCNT
- sperm
- vitrificaction
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