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Associate Professor Steve Johnston
Associate Professor

Steve Johnston

Email: 
Phone: 
+61 7 54601 076

Overview

Background

A/Prof Stephen Johnston is a Reader in Reproduction (40% Teaching; 40% Research and 20% Service) in the School of Environment at the University of Queensland, where he teaches animal reproduction and captive husbandry to Bachelor of Wildlife Science and Science undergraduate and postgraduate students. He is current program coordinator for the Bachelor of Wildlife Science Program. He is also a research affiliate in the School of Veterinary Science. Stephen was trained as a zoologist and specialises in the area of reproductive biology in a broad diversity of species ranging from prawns to tigers but with a major focus on Australian mammals. A/Prof Johnston has published 300 scientific works in basic and applied science disciplines including reproductive anatomy, physiology and behaviour. He was the first person in the world to produce a pouch young following artificial insemination in a marsupial, a task that he and his colleagues have now carried out successfully in the koala, a total of 34 times. Stephen is also a specialist in the cryopreservation of marsupial spermatozoa and in the assessment of sperm DNA fragmentation, including human and domestic animal spermatozoa. A/Prof Johnston’s recent research interests and grant success include studies aimed at a better understanding of the effect of chlamydia on male koala reproduction, heat stress in koalas, sociobiology of koalas, genetic and reproductive management of koalas, wombat captive reproduction, echidna captive breeding, crocodile artificial insemination and prawn aquaculture. He is a Fellow of the Society for Reproductive Biology (2019)

Availability

Associate Professor Steve Johnston is:
Not available for supervision
Media expert

Qualifications

  • Bachelor (Honours) of Science (Advanced), The University of Queensland
  • Doctor of Philosophy, The University of Queensland

Research interests

  • Reproductive Biology of Wildlife

    The study of fundamental (anatomy, physiology and behaviour) and applied biology of the reproduction of Australian fauna and exotic mammals

  • Assessment of male human fertility

    Studies centred around the assessment of sperm DNA fragmentation in human reproductive medicine

  • Cryopreservation of spermatozoa

    A study of the cryopreservation of spermatozoa of vertebrate species

Research impacts

For 25 years, A/Prof Johnston has been the leader in the development of assisted breeding technology in Australian native fauna, with a strong interest in marsupial reproduction. His successful koala artificial breeding technology has been incorporated into State Government Conservation Policy and Legislation. The koala assisted breeding technology, which he developed, is also currently been used by the Queensland Zoo industry for genetic exchange and there are plans to use the technology for the overseas export of koala sperm to Japan and the United States. This work has also received recent acknowledgement n the Koala Expert Panel Final Report to the Queensland Government (2018). He provides expert advice and consultancy to the (1) Zoo Industry - Dreamworld, Australia Zoo, Currumbin Wildlife Sanctuary, Taronga Zoo, Lone Pine Koala Sanctuary, Rockhampton Zoo, Perth Zoo, Australian Animals Care and Education, Sea World, Paradise Country, San Diego Zoo, Australian Japan Wildlife Education Conservation Foundation, Japanese Zoo Industry (2) Production Animal Industry - Koorana Crocodile Farm, Ag Force – teacher education programs, Prawn and Aquaculture (DAFFI and CSIRO). He is a regular media commentator on wildlife related issues for TV and radio and has been involved with numerous documentaries on marsupial and monotreme reproduction including “Koala Miracle” by Lyndal Davies (1998) from Rock Wallaby Productions, (which featured his work on the successful artificial insemination of the koala) “Miracle babies” produced by National Geographic Channel (2012) - http://channel.nationalgeographic.com/wild/episodes/koala-dating-game1/ and morerecently, a new documentary “Born in the Wild” produced by Charlotte Hunt-Grubble from Windfall Films on my koala and echidna reproductive research.

Works

Search Professor Steve Johnston’s works on UQ eSpace

305 works between 1992 and 2025

1 - 20 of 305 works

2025

Journal Article

Factors influencing scrotal temperature in the bull during simulated heat treatments and thermoneutral conditions as determined by multimodel inferencing

Wallage, A. L., Lees, A. M., Lisle, A. T., Lees, J. C., Johnston, S. D. and Gaughan, J. B. (2025). Factors influencing scrotal temperature in the bull during simulated heat treatments and thermoneutral conditions as determined by multimodel inferencing. Journal of Thermal Biology, 127 104037, 104037. doi: 10.1016/j.jtherbio.2024.104037

Factors influencing scrotal temperature in the bull during simulated heat treatments and thermoneutral conditions as determined by multimodel inferencing

2025

Journal Article

Observations on the reproductive morphology of the female short-beaked echidna, Tachyglossus aculeatus

Fenelon, Jane C., Ferrier, Stephanie B., Johnston, Stephen D. and Renfree, Marilyn B. (2025). Observations on the reproductive morphology of the female short-beaked echidna, Tachyglossus aculeatus. Journal of Anatomy, 246 (1), 120-133. doi: 10.1111/joa.14142

Observations on the reproductive morphology of the female short-beaked echidna, Tachyglossus aculeatus

2024

Conference Publication

28 Successful production of kangaroo ICSI embryos

Palacios, P. D., Gurkin, R. J., Campbell, Y., Zhao, J., Pini, T., Johnston, S. and Gambini, A. (2024). 28 Successful production of kangaroo ICSI embryos. Annual Conference of the International Embryo Technology Society, Fort Worth, TX United States, 18–22 January 2025. Clayton, VIC Australia: CSIRO Publishing. doi: 10.1071/rdv37n1ab28

28 Successful production of kangaroo ICSI embryos

2024

Journal Article

Strong correlation between double-strand DNA Breaks and total sperm DNA fragmentation in the human ejaculate

Gosálvez, Jaime, Johnston, Stephen D., Prado, Ahinoa, López-Fernández, Carmen, Contreras, Pablo, Bartolomé-Nebreda, Javier, González-Martínez, Mercedes, Fernández, José Luis, de la Vega, Carlos García and Góngora, Alfredo (2024). Strong correlation between double-strand DNA Breaks and total sperm DNA fragmentation in the human ejaculate. Archives of Medical Research, 55 (8) 103122, 1-8. doi: 10.1016/j.arcmed.2024.103122

Strong correlation between double-strand DNA Breaks and total sperm DNA fragmentation in the human ejaculate

2024

Journal Article

Semen collection and baseline semen characteristics of the king quail (Synoicus chinesis)

Wong, Isabella, Doneley, Robert and Johnston, Stephen (2024). Semen collection and baseline semen characteristics of the king quail (Synoicus chinesis). Theriogenology Wild, 5 100117, 1-9. doi: 10.1016/j.therwi.2024.100117

Semen collection and baseline semen characteristics of the king quail (Synoicus chinesis)

2024

Journal Article

Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation

Damyanova, Katerina B., Nixon, Brett, Johnston, Stephen D., Gambini, Andrés, Benitez, Patricio P. and Lord, Tessa (2024). Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation. Biology of Reproduction, 111 (4), 757-779. doi: 10.1093/biolre/ioae109

Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation

2024

Journal Article

The utility of the koala scat: a scoping review

Johnston, Stephen D., Hulse, Lyndal, Keeley, Tamara, Mucci, Albano, Seddon, Jennifer and Maynard, Sam (2024). The utility of the koala scat: a scoping review. Biology, 13 (7) 523, 523. doi: 10.3390/biology13070523

The utility of the koala scat: a scoping review

2024

Journal Article

Immunohistochemical characterization of the immune cell response during chlamydial infection in the male and female koala (Phascolarctos cinereus) reproductive tract

Pagliarani, Sara, Johnston, Stephen D., Beagley, Kenneth W. and Palmieri, Chiara (2024). Immunohistochemical characterization of the immune cell response during chlamydial infection in the male and female koala (Phascolarctos cinereus) reproductive tract. Veterinary Pathology, 61 (4), 621-632. doi: 10.1177/03009858231225499

Immunohistochemical characterization of the immune cell response during chlamydial infection in the male and female koala (Phascolarctos cinereus) reproductive tract

2024

Journal Article

Body fat and circulating leptin levels in the captive short-beaked echidna (Tachyglossus aculeatus)

Dutton-Regester, Kate J., Roser, Alice, Meer, Haley, Hill, Andrew, Pyne, Michael, Al-Najjar, Aiman, Whaites, Tim, Fenelon, Jane C., Buchanan, Katherine L., Keeley, Tamara, Renfree, Marilyn B. and Johnston, Stephen D. (2024). Body fat and circulating leptin levels in the captive short-beaked echidna (Tachyglossus aculeatus). Journal of Comparative Physiology B, 194 (4), 1-15. doi: 10.1007/s00360-024-01559-z

Body fat and circulating leptin levels in the captive short-beaked echidna (Tachyglossus aculeatus)

2024

Journal Article

The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation, and copy number in normal and abnormal human ejaculates

Tímermans, Ana, Otero, Fátima, Garrido, Manuel, Gosálvez, Jaime, Johnston, Stephen and Fernández, José Luis (2024). The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation, and copy number in normal and abnormal human ejaculates. Andrology, 12 (4), 870-880. doi: 10.1111/andr.13539

The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation, and copy number in normal and abnormal human ejaculates

2024

Journal Article

Role of DNase activity in human sperm DNA fragmentation

Gosálvez, Jaime, Fernández, Carmen López, Johnston, Stephen D. and Bartolomé-Nebreda, Javier (2024). Role of DNase activity in human sperm DNA fragmentation. Biomolecules, 14 (3) 304, 1-12. doi: 10.3390/biom14030304

Role of DNase activity in human sperm DNA fragmentation

2024

Other Outputs

Complete multimodel dataset

Lees, Angela, Gaughan, John, Wallage, Andrea and Johnston, Steve (2024). Complete multimodel dataset. The University of Queensland. (Dataset) doi: 10.48610/fb62e47

Complete multimodel dataset

2024

Journal Article

The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa

González-Martínez, Mercedes, Sánchez-Martín, Pascual, López-Fernández, Carmen, Johnston, Stephen D. and Gosálvez, Jaime (2024). The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa. Asian Pacific Journal of Reproduction, 13 (1), 22-27. doi: 10.4103/apjr.apjr_42_23

The relationship between DNA fragmentation and the intensity of morphologically abnormal human spermatozoa

2023

Conference Publication

Koala sperm induces bovine oocyte activation after intracytoplasmic sperm injection

Palacios Benitez, P. D., Duncan, N., Nilesh Haldankar, A., Johnston, S. and Gambini, A. (2023). Koala sperm induces bovine oocyte activation after intracytoplasmic sperm injection. Annual Conference of the International Embryo Technology Society 2024, Denver, CO USA, 9-12 January 2024. Clayton, VIC Australia: CSIRO Publishing. doi: 10.1071/rdv36n2ab35

Koala sperm induces bovine oocyte activation after intracytoplasmic sperm injection

2023

Journal Article

Gut microbiota in the short‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation

Buthgamuwa, Isini, Fenelon, Jane C., Roser, Alice, Meer, Haley, Johnston, Stephen D. and Dungan, Ashley M. (2023). Gut microbiota in the short‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation. MicrobiologyOpen, 12 (6) e1392, e1392. doi: 10.1002/mbo3.1392

Gut microbiota in the short‐beaked echidna (Tachyglossus Aculeatus) shows stability across gestation

2023

Journal Article

Addendum to: The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation and copy number in normal and abnormal human ejaculates

Tímermans, Ana, Otero, Fátima, Garrido, Manuel, Gosálvez, Jaime, Johnston, Stephen and Fernández, José Luis (2023). Addendum to: The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation and copy number in normal and abnormal human ejaculates. Andrology, 12 (5), 1111-1118. doi: 10.1111/andr.13559

Addendum to: The relationship between sperm nuclear DNA fragmentation, mitochondrial DNA fragmentation and copy number in normal and abnormal human ejaculates

2023

Journal Article

Anatomy of the female koala reproductive tract

Pagliarani, Sara, Palmieri, Chiara, McGowan, Michael, Carrick, Frank, Boyd, Jackson and Johnston, Stephen D. (2023). Anatomy of the female koala reproductive tract. Biology, 12 (11) 1445, 1-34. doi: 10.3390/biology12111445

Anatomy of the female koala reproductive tract

2023

Journal Article

An evaluation of DNA sample source and molecular markers to determine gender in the short-beaked echidna (Tachyglossus aculeatus)

Hulse, Lyndal, Tomsett, Caitlin, Roser, Alice, Clark, Lauren, Meer, Haley, Pyne, Michael, Fenelon, Jane C, Renfree, Marilyn B and Johnston, Stephen (2023). An evaluation of DNA sample source and molecular markers to determine gender in the short-beaked echidna (Tachyglossus aculeatus). Zoo Biology, 43 (1), 92-99. doi: 10.1002/zoo.21809

An evaluation of DNA sample source and molecular markers to determine gender in the short-beaked echidna (Tachyglossus aculeatus)

2023

Journal Article

The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation

Campbell, Yolande, Palmieri, Chiara, Pagliarani, Sara, Gordon, Jo and Johnston, Stephen (2023). The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation. Biology of Reproduction, 109 (5), 644-653. doi: 10.1093/biolre/ioad098

The koala (Phascolarctos cinereus) prostate: a comprehensive histological and immunohistochemical investigation

2023

Journal Article

Getting out of a mammalian egg: the egg tooth and caruncle of the echidna

Fenelon, Jane C., Bennetts, Abbie, Anthwal, Neal, Pyne, Michael, Johnston, Stephen D., Evans, Alistair R., Tucker, Abigail S. and Renfree, Marilyn B. (2023). Getting out of a mammalian egg: the egg tooth and caruncle of the echidna. Developmental Biology, 495, 8-18. doi: 10.1016/j.ydbio.2022.12.005

Getting out of a mammalian egg: the egg tooth and caruncle of the echidna

Funding

Current funding

  • 2024 - 2029
    Koala Health and Rehabilitation Collaborative for research to advance koala reproductive health and promote species survival
    Koala Health and Rehabilitation Collaborative
    Open grant
  • 2023 - 2026
    UQ Koala Research Fellowship
    Open grant

Past funding

  • 2022 - 2024
    Do environmental stressors increase retrovirus activity and exacerbate disease in koalas?
    New South Wales Department of Planning and Environment
    Open grant
  • 2022 - 2024
    The Power of Koala Poo
    Celestino Pty Ltd
    Open grant
  • 2019 - 2020
    Identification of key players for the outcome of Chlamydia infection in female koalas
    Dreamworld
    Open grant
  • 2019 - 2020
    Automated immunohistochemical analysis module for the expansion of research and clinical diagnostic capabilities at UQ
    UQ Major Equipment and Infrastructure
    Open grant
  • 2019
    The immune response to Chlamydia pecorum infection in the reproductive tract of female koalas
    Annandale Bequest
    Open grant
  • 2018 - 2022
    Koala retrovirus epidemic: genetic diversity, genome invasion and disease
    ARC Discovery Projects
    Open grant
  • 2017 - 2021
    Breeding echidnas in captivity (ARC Linkage Project administered by The University of Melbourne)
    University of Melbourne
    Open grant
  • 2017 - 2018
    Chlamydia infiection in the ovary: effects on fertility in female koalas
    Annandale Bequest
    Open grant
  • 2017 - 2021
    A Living Koala Genome Bank
    Queensland Government Advance Queensland Innovation Partnerships
    Open grant
  • 2014 - 2015
    Computer Modelling of the anatomy of the Northern Hairy-Nosed Wombat (Lasiorhinus kreftii): A New Dimension in Knowledge, Understanding and Awareness
    The Wombat Foundation
    Open grant
  • 2014
    Establishment of a core facility in reproductive technology including cell micromanipulation and stem cells to improve research capacity and benefit animal health at the Gatton campus
    UQ Major Equipment and Infrastructure
    Open grant
  • 2013 - 2017
    Behavioural and physiological adaptations to heat in the koala
    Queensland Department of Environment and Heritage Protection
    Open grant
  • 2013 - 2015
    Pathways of pathogen transmission in the koala
    Queensland Department of Environment and Heritage Protection
    Open grant
  • 2013 - 2017
    The pathology, incidence, treatment and management of Chlamydiosis in the male koala.
    Queensland Department of Environment and Heritage Protection
    Open grant
  • 2013 - 2020
    Genetic Capture Technology in the Conservation of the Koala in South-East Queensland
    Dreamworld
    Open grant
  • 2011 - 2013
    Development of assisted breeding techniques in the crocodile industry
    Rural Industries Research & Development Corporation
    Open grant
  • 2010 - 2011
    Artificial insemination of the southern hairy-nosed wombat
    The Wombat Foundation
    Open grant
  • 2010 - 2011
    Gamete recovery from diseased koalas
    San Diego Zoo
    Open grant
  • 2010 - 2011
    Gamete recovery from diseased koalas
    Open grant
  • 2010 - 2011
    Use of the GnRH antagonist AZALINE B to control koala anterior pituitary function and oestrus synchronisation
    Dreamworld
    Open grant
  • 2010
    UQ Travel Awards #2 - Stephen Johnston
    UQ Travel Grants Scheme
    Open grant
  • 2009
    Deltavision Core deconvolution microscope
    UQ School/Centre Co-Funding
    Open grant
  • 2007 - 2009
    Australian Wildlife on the Web: a web resource supporting undergraduate animal behavioural studies students
    UQ Teaching & Learning Strategic Grants
    Open grant
  • 2007 - 2008
    Reproductive biology of the greater bilby Macrotis lagotis - Dreamworld
    Dreamworld
    Open grant
  • 2005 - 2007
    The Captive Management of Southern Hairy Nosed Wombats at Rockhampton Zoo
    The Wombat Foundation
    Open grant
  • 2005 - 2006
    The University of Queensland Echidna Study Program: A world class facility for collaborative echidna research
    UQ Foundation Research Excellence Awards - DVC(R) Funding
    Open grant
  • 2005 - 2008
    Reproductive Biology and Contraception in the Australian White Ibis Research Program 2005 - 2008
    Brisbane City Council
    Open grant
  • 2005 - 2008
    The Preservation And Management Of Koala Genetic Diversity Using Reproductive Biotechnology And Molecular Genetics: A Model For Endangered Australian Marsupials
    ARC Linkage Projects
    Open grant
  • 2003
    Reproductive technology for the aviculture industry: A model for breeding endangered parrots
    University of Queensland Research Development Grants Scheme
    Open grant
  • 2002 - 2005
    A model for the development of ex situ conservation of Papua New Guinea's critically endangered long-beaked echidna
    Currumbin Wildlife Sanctuary
    Open grant
  • 2000
    Reproductive Endocrinology of the Koala: Evidence for a Unique Pattern of Marsupial Reproduction.
    UQ Early Career Researcher
    Open grant
  • 1999
    Investigating improved methods for the cryopreservation of marsupial spermatozoa: establishing a research programme
    UQ New Staff Research Start-Up Fund
    Open grant

Supervision

Availability

Associate Professor Steve Johnston is:
Not available for supervision

Supervision history

Current supervision

  • Doctor Philosophy

    Comparative Anatomy and Physiology of Monotreme and Marsupial Accessory Glands

    Principal Advisor

    Other advisors: Professor Chiara Palmieri, Dr Taylor Pini

  • Doctor Philosophy

    Novel strategies for marsupial genetic preservation and in vitro embryo production

    Associate Advisor

    Other advisors: Dr Andres Gambini

  • Doctor Philosophy

    Novel methods for improving in vitro production of bovine embryos

    Associate Advisor

    Other advisors: Dr Andres Gambini

  • Doctor Philosophy

    Hatching out the secrets of female monotreme reproductive biology

    Associate Advisor

    Other advisors: Professor Chiara Palmieri, Dr Taylor Pini

Completed supervision

Media

Enquiries

Contact Associate Professor Steve Johnston directly for media enquiries about:

  • Animal reproduction
  • Bilbies
  • Breeding technologies - for animals
  • Captive breeding programs
  • Crocodile reproduction
  • Cryopreservation - animal genetics
  • Embryo transfer - animals
  • Exotic animals - reproductive biology
  • IVF - for animals
  • Kangaroos
  • Koalas
  • Koalas - reproductive biology
  • Reproduction in animals
  • Rhinos
  • Spermatology - animal studies
  • Wildlife reproductive biology
  • Wombats
  • Zoo captive breeding

Need help?

For help with finding experts, story ideas and media enquiries, contact our Media team:

communications@uq.edu.au