
Overview
Background
I am interested in ion channels in health and disease. Improved technologies for genetic screening is revealing an expanding catalogue of genetic variants to ion channels that give rise neurological disorders, such as epilespy syndromes, autism spectrum disorder and other neurodevelopmental disorders. My main research focus is on neurotransmitter-activated receptor ion channels that are found at neuronal synapses. These include GABA-A, glycine and glutamate (NMDA) receptors. I also work on other ion channesl such as voltage-gated sodium channels and synaptic receptors expressed in invertebrate nervous systems.
I am an expert at recording single ion channel, synaptic and conventional whole-cell currents for functional and pharmacological analyisis.
I collaborate with biophysicists, molecular biologists, geneticists, electrophysiologists and clinicians that specialise in genetic neurological disorders. I have active projects in collaboration with research groups in Australia, USA and Europe.
Availability
- Dr Angelo Keramidas is:
- Available for supervision
Fields of research
Qualifications
- Bachelor of Science, Institution to be confirmed
- Doctor of Philosophy, Institution to be confirmed
Research impacts
I am collaborating with basic and clinical research groups that bring together a great diversity of research techniques with the common aim of developing personalised medicine for affected individuals with neurological disorders.
Works
Search Professor Angelo Keramidas’s works on UQ eSpace
2017
Journal Article
Effects of glutamate and ivermectin on single glutamate-gated chloride channels of the parasitic nematode H. contortus
Atif, Mohammed, Estrada-Mondragon, Argel, Nguyen, Bindi, Lynch, Joseph W. and Keramidas, Angelo (2017). Effects of glutamate and ivermectin on single glutamate-gated chloride channels of the parasitic nematode H. contortus. PLoS Pathogens, 13 (10) e1006663, e1006663. doi: 10.1371/journal.ppat.1006663
2017
Journal Article
Physiological and pharmacological properties of inhibitory postsynaptic currents mediated by α5β1γ2, α5β2γ2 and α5β3γ2 GABAA receptors
Chen, Xiumin, Keramidas, Angelo and Lynch, Joseph W. (2017). Physiological and pharmacological properties of inhibitory postsynaptic currents mediated by α5β1γ2, α5β2γ2 and α5β3γ2 GABAA receptors. Neuropharmacology, 125, 243-253. doi: 10.1016/j.neuropharm.2017.07.027
2017
Journal Article
Gamma 1-containing GABA-A receptors cluster at synapses where they mediate slower synaptic currents than gamma 2-containing GABA-A receptors
Dixon, Christine L., Sah, Pankaj, Keramidas, Angelo, Lynch, Joseph W. and Durisic, Nela (2017). Gamma 1-containing GABA-A receptors cluster at synapses where they mediate slower synaptic currents than gamma 2-containing GABA-A receptors. Frontiers in Molecular Neuroscience, 10 178, 178. doi: 10.3389/fnmol.2017.00178
2017
Journal Article
Structure-function analysis of the GlyR alpha 2 subunit autism mutation p.R323L reveals a gain-of-function
Zhang, Yan, Ho, Thi Nhu Thao, Harvey, Robert J., Lynch, Joseph W. and Keramidas, Angelo (2017). Structure-function analysis of the GlyR alpha 2 subunit autism mutation p.R323L reveals a gain-of-function. Frontiers in Molecular Neuroscience, 10 158, 158. doi: 10.3389/fnmol.2017.00158
2017
Journal Article
Probing the structural mechanism of partial agonism in glycine receptors using the fluorescent artificial amino acid, ANAP
Soh, Ming S., Estrada-Mondragon, Argel, Durisic, Nela, Keramidas, Angelo and Lynch, Joseph W. (2017). Probing the structural mechanism of partial agonism in glycine receptors using the fluorescent artificial amino acid, ANAP. ACS Chemical Biology, 12 (3), 805-813. doi: 10.1021/acschembio.6b00926
2017
Journal Article
Potent neuroprotection after stroke afforded by a double-knot spider-venom peptide that inhibits acid-sensing ion channel 1a
Chassagnon, Irene R., McCarthy, Claudia A., Chin, Yanni K.-Y., Pineda, Sandy S., Keramidas, Angelo, Mobli, Mehdi, Pham, Vi, De Silva, T. Michael, Lynch, Joseph W., Widdop, Robert E., Rash, Lachlan D. and King, Glenn F. (2017). Potent neuroprotection after stroke afforded by a double-knot spider-venom peptide that inhibits acid-sensing ion channel 1a. Proceedings from the National Academy of Sciences of the United States of America, 114 (14), 3750-3755. doi: 10.1073/pnas.1614728114
2017
Journal Article
Multiple sodium channel isoforms mediate the pathological effects of Pacific ciguatoxin-1
Inserra, Marco C., Isreal, Mathilde, Caldwell, Ashlee, Castro, Joel, Deuis, Jennifer R., Harrington, Andrea M., Keramidas, Angelo, Garcia-Caraballo, Sonia, Maddern, Jessica, Erickson, Andelain, Grundy, Luke, Rychkov, Grigori Y., Zimmerman, Katharina, Lewis, Richard J., Brierley, Stuart M. and Vetter, Irina (2017). Multiple sodium channel isoforms mediate the pathological effects of Pacific ciguatoxin-1. Scientific Reports, 7 (1) 42810, 42810. doi: 10.1038/srep42810
2016
Journal Article
Ivermectin-activated, cation-permeable glycine receptors for the chemogenetic control of neuronal excitation
Islam, Robiul, Keramidas, Angelo, Xu, Li, Durisic, Nela, Sah, Pankaj and Lynch, Joseph W. (2016). Ivermectin-activated, cation-permeable glycine receptors for the chemogenetic control of neuronal excitation. ACS Chemical Neuroscience, 7 (12), 1647-1657. doi: 10.1021/acschemneuro.6b00168
2016
Journal Article
The free zinc concentration in the synaptic cleft of artificial glycinergic synapses rises to at least 1 µm
Zhang, Yan, Keramidas, Angelo and Lynch, Joseph W. (2016). The free zinc concentration in the synaptic cleft of artificial glycinergic synapses rises to at least 1 µm. Frontiers in Molecular Neuroscience, 9 (SEP2016) 88, 88. doi: 10.3389/fnmol.2016.00088
2016
Journal Article
Investigating the mechanism by which gain-of-function mutations to the a1 glycine receptor cause hyperekplexia
Zhang, Yan, Bode, Anna, Nguyen, Bindi, Angelo Keramidas and Lynch, Joseph W. (2016). Investigating the mechanism by which gain-of-function mutations to the a1 glycine receptor cause hyperekplexia. Journal of Biological Chemistry, 291 (29), 15332-15341. doi: 10.1074/jbc.M116.728592
2015
Journal Article
Correlating structural and energetic changes in glycine receptor activation
Scott, Suzanne, Lynch, Joseph W and Keramidas, Angelo (2015). Correlating structural and energetic changes in glycine receptor activation. Journal of Biological Chemistry, 290 (9), 5621-5634. doi: 10.1074/jbc.M114.616573
2015
Journal Article
Zolpidem and eszopiclone prime α1β2γ2 GABAA receptors for longer duration of activity
Dixon, Christine L., Harrison, Neil L., Lynch, Joseph W. and Keramidas, Angelo (2015). Zolpidem and eszopiclone prime α1β2γ2 GABAA receptors for longer duration of activity. British Journal of Pharmacology, 172 (14), 3522-3536. doi: 10.1111/bph.13142
2015
Journal Article
Functional reconstitution of glycinergic synapses incorporating defined glycine receptor subunit combinations
Zhang, Yan, Dixon, Christine L., Keramidas, Angelo and Lynch, Joseph W. (2015). Functional reconstitution of glycinergic synapses incorporating defined glycine receptor subunit combinations. Neuropharmacology, 89, 391-397. doi: 10.1016/j.neuropharm.2014.10.026
2014
Journal Article
GABAa receptor α and γ subunits shape synaptic currents via different mechanisms
Dixon, Christine, Sah, Pankaj, Lynch, Joseph W. and Keramidas, Angelo (2014). GABAa receptor α and γ subunits shape synaptic currents via different mechanisms. Journal of Biological Chemistry, 289 (9), 5399-5411. doi: 10.1074/jbc.M113.514695
2013
Journal Article
New hyperekplexia mutations provide insight into glycine receptor assembly, trafficking, and activation mechanisms
Bode, Anna, Wood, Sian-Elin, Mullins, Jonathan G. L., Keramidas, Angelo, Cushion, Thomas D., Thomas, Rhys H., Pickrell, William O., Drew, Cheney J. G., Masri, Amira, Jones, Elizabeth A., Vassallo, Grace, Born, Alfred P., Alehan, Fusun, Aharoni, Sharon, Bannasch, Gerald, Bartsch, Marius, Kara, Bulent, Krause, Amanda, Karam, Elie G., Matta, Stephanie, Jain, Vivek, Mandel, Hanna, Freilinger, Michael, Graham, Gail E., Hobson, Emma, Chatfield, Sue, Vincent-Delorme, Catherine, Rahme, Jubran E., Afawi, Zaid ... Lynch, Joseph W. (2013). New hyperekplexia mutations provide insight into glycine receptor assembly, trafficking, and activation mechanisms. Journal of Biological Chemistry, 288 (47), 33745-33759. doi: 10.1074/jbc.M113.509240
2013
Journal Article
Novel missense mutations in the glycine receptor β subunit gene (GLRB) in startle disease
James, Victoria M., Bode, Anna, Chung, Seo-Kyung, Gill, Jennifer L., Nielsen, Maartje, Cowan, Frances M., Vujic, Mihailo, Thomas, Rhys H., Rees, Mark I., Harvey, Kirsten, Keramidas, Angelo, Topf, Maya, Ginjaar, Ieke, Lynch, Joseph W. and Harvey, Robert J. (2013). Novel missense mutations in the glycine receptor β subunit gene (GLRB) in startle disease. Neurobiology of Disease, 52, 137-149. doi: 10.1016/j.nbd.2012.12.001
2012
Journal Article
An outline of desensitization in pentameric ligand-gated ion channel receptors
Keramidas, Angelo and Lynch, Joseph W. (2012). An outline of desensitization in pentameric ligand-gated ion channel receptors. Cellular and Molecular Life Sciences, 70 (7), 1241-1253. doi: 10.1007/s00018-012-1133-z
2010
Journal Article
The activation mechanism of alpha(1)beta(2)gamma(2S) and alpha(3)beta(3)gamma(2S) GABA(A) receptors
Keramidas, Angelo and Harrison, Neil L. (2010). The activation mechanism of alpha(1)beta(2)gamma(2S) and alpha(3)beta(3)gamma(2S) GABA(A) receptors. Journal of General Physiology, 135 (1), 59-75. doi: 10.1085/jgp.200910317
2008
Conference Publication
Agonist-dependent single channel current and Gating in alpha(4)beta(2)delta and alpha(1)beta(2)gamma(2S) GABA(A) receptors
Keramidas, Angelo and Harrison, Neil L. (2008). Agonist-dependent single channel current and Gating in alpha(4)beta(2)delta and alpha(1)beta(2)gamma(2S) GABA(A) receptors. New York, United States: Rockefeller University Press. doi: 10.1085/jgp.200709871
2008
Journal Article
Taurine is a potent activator of extrasynaptic GABAA receptors in the thalamus
Jia, Fan, Yue, Minerva, Chandra, Dev, Keramidas, Angelo, Goldstein, Peter A., Homanics, Gregg E. and Harrison, Neil L. (2008). Taurine is a potent activator of extrasynaptic GABAA receptors in the thalamus. Journal of Neuroscience, 28 (1), 106-115. doi: 10.1523/JNEUROSCI.3996-07.2008
Funding
Current funding
Past funding
Supervision
Availability
- Dr Angelo Keramidas is:
- Available for supervision
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Available projects
-
GABA-A and glycine receptor variants in epilepsy and autism
New gene variants identified by our clinical collaborators to receptors that mediate neuronal inhibition. These variants lead to receptor functional deficits that manifest as neurological syndromes in young affected individuals.
We aim to characterise the functional deficists in these receptors and explore personalised treatment options that are tailored to each receptor variant.
-
Glutamate (NMDA) receptors in develpmental delay
Genetic variants to glutamate receptors have been discovered by our clinical geneticists. We aim the understand how these variants lead to developmental delay and co-morbid disorders in affected individuals and explore pharmacotherapies that correct the functional deficits in these important receptors.
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Tuning the activating stimulus of voltage-gated sodium channels
This project will use natural and modified peptides that are derived from venoms of different species, such as spiders and ants to probe and manipulate the functional properties of voltage-gated ion channels, which are critically important to the function of the nervous system.
You will learn patch-clamp electrophysiology (whole-cell and single channels) and how to isolate, synthesise and determine the structure of venom peptides and their mechanisms of action at ion channels.
Supervision history
Current supervision
-
Doctor Philosophy
Probing ion channel function using venom peptides
Principal Advisor
Other advisors: Professor Irina Vetter
-
Doctor Philosophy
Investigating ion channel function and pathology using toxins as tools
Associate Advisor
Other advisors: Dr Jennifer Deuis, Professor Irina Vetter
Completed supervision
-
2019
Doctor Philosophy
Glutamate-gated chloride channel receptors and mechanisms of drug resistance in pathogenic species
Joint Principal Advisor
-
2022
Master Philosophy
Investigating the role of the novel GABAA receptor variant, ß2(L51M), in the pathophysiology of epilepsy
Associate Advisor
Other advisors: Professor Irina Vetter
-
2019
Master Philosophy
Characterization of novel synthetic analgesics selectively targeting Alpha3 Glycine receptors
Associate Advisor
-
2019
Doctor Philosophy
Pharmacological and physiological investigation of GABAA and NMDA receptors containing epilepsy mutations
Associate Advisor
-
2017
Doctor Philosophy
Physiological properties of glycinergic synapses with defined subunit compositions
Associate Advisor
Other advisors: Professor Pankaj Sah
-
2016
Doctor Philosophy
Discovering novel alpha5-containing GABA-A receptor selective drugs for schizophrenia, stroke and cognitive impairments
Associate Advisor
-
2015
Doctor Philosophy
Identification and characterization of new synthetic drugs selectively targeting alpha3 glycine receptors as therapeutic leads for pain treatment
Associate Advisor
-
2014
Doctor Philosophy
Effects of new Hyperekplexia Mutations on Human Glycine Receptor Structure and Function
Associate Advisor
-
2013
Doctor Philosophy
Voltage-Clamp Fluorometry of Glycine Receptors: Relevance to Pain and Startle Disease
Associate Advisor
Media
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