Overview
Availability
- Associate Professor Anne Lagendijk is:
- Available for supervision
Qualifications
- Doctor of Philosophy, Utrecht University
Research interests
-
Cellular mechanisms to maintain a healthy vasculature
Our vascular system transports approximately 7500 liters of blood each day. Arteries deliver oxygen and nutrients throughout the body after which the venous system returns the deoxygenated blood back to the heart. Architecturally, these blood vessels are extremely heterogeneous. The Lagendijk group investigates the development and maintenance of a functional blood vessel network in zebrafish and bioengineered human microvessels. The cells that make up our blood vessels continuously adapt their size, adhesiveness and compliance order to ensure the right balance between vessel integrity and permeability in a context dependent manner. Mechanical cues play a major role in the functional adaptation of blood vessels. Despite ongoing research unraveling the structural components of mechanical hubs in the cells, it is essential to assess the magnitude of forces that are transduced at these sites and the biological consequences for vessel function. Dr. Lagendijk has previously developed a VE-cadherin tension biosensor line in zebrafish. This line provides the first vertebrate model that reports intra-molecular tension and was utilised to identify changes in junctional organisation and VE-cadherin tension that occur as arteries mature and revealed molecular pathways that allow for this maturation to happen. In addition, the lab has established disease models for vascular malformations that are known to lead to neurological deficits and stroke. Modelling in zebrafish allows analyses of the initiating mechanisms of these vascular pathologies at unprecedented cellular and subcellular resolution.
Works
Search Professor Anne Lagendijk’s works on UQ eSpace
2013
Journal Article
A Nodal-independent and tissue-intrinsic mechanism controls heart-looping chirality
Noel, Emily S., Verhoeven, Manon, Lagendijk, Anne Karine, Tessadori, Federico, Smith, Kelly, Choorapoikayil, Suma, Den Hertog, Jeroen and Bakkers, Jeroen (2013). A Nodal-independent and tissue-intrinsic mechanism controls heart-looping chirality. Nature Communications, 4 (1) 2754. doi: 10.1038/ncomms3754
2012
Journal Article
Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues
Lagendijk, A. K., Moulton, J. D. and Bakkers, J. (2012). Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open, 1 (6), 566-569. doi: 10.1242/bio.2012810
2012
Journal Article
Bmp signaling exerts opposite effects on cardiac differentiation
de Pater, Emma, Ciampricotti, Metamia, Priller, Florian, Veerkamp, Justus, Strate, Ina, Smith, Kelly, Lagendijk, Anne Karine, Schilling, Thomas F., Herzog, Wiebke, Abdelilah-Seyfried, Salim, Hammerschmidt, Matthias and Bakkers, Jeroen (2012). Bmp signaling exerts opposite effects on cardiac differentiation. Circulation Research, 110 (4), 578-587. doi: 10.1161/CIRCRESAHA.111.261172
2011
Journal Article
Transmembrane protein 2 (Tmem2) is required to regionally restrict atrioventricular canal boundary and endocardial cushion development
Smith, Kelly A., Lagendijk, Anne K., Courtney, Andrew D., Chen, Huijun, Paterson, Scott, Hogan, Benjamin M., Wicking, Carol and Bakkers, Jeroen (2011). Transmembrane protein 2 (Tmem2) is required to regionally restrict atrioventricular canal boundary and endocardial cushion development. Development, 138 (19), 4193-4198. doi: 10.1242/dev.065375
2011
Journal Article
MicroRNA-23 restricts cardiac valve formation by inhibiting has2 and extracellular hyaluronic acid production
Lagendijk, Anne Karine, Goumans, Marie Jose, Burkhard, Silja Barbara and Bakkers, Jeroen (2011). MicroRNA-23 restricts cardiac valve formation by inhibiting has2 and extracellular hyaluronic acid production. Circulation Research, 109 (6), 649-657. doi: 10.1161/CIRCRESAHA.111.247635
2011
Journal Article
Macrophage development from HSCs requires PU.1-coordinated microRNA expression
Ghani, Saeed, Riemke, Pia, Schönheit, Jörg, Lenze, Dido, Stumm, Jürgen, Hoogenkamp, Maarten, Lagendijk, Anne, Heinz, Sven, Bonifer, Constanze, Bakkers, Jeroen, Abdelilah-Seyfried, Salim, Hummel, Michael and Rosenbauer, Frank (2011). Macrophage development from HSCs requires PU.1-coordinated microRNA expression. Blood, 118 (8), 2275-2284. doi: 10.1182/blood-2011-02-335141
2010
Journal Article
Genetics of congenital heart defects: A candidate gene approach
Lagendijk, Anne Karine, Smith, Kelly A. and Bakkers, Jeroen (2010). Genetics of congenital heart defects: A candidate gene approach. Trends in Cardiovascular Medicine, 20 (4), 124-128. doi: 10.1016/j.tcm.2010.10.003
2009
Journal Article
Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model
Marques, Ines J., Weiss, Frank U., Vlecken, Danielle H., Nitsche, Claudia, Bakkers, Jeroen, Lagendijk, Anne K., Partecke, Lars I., Heidecke, Claus-Dieter, Lerch, Markus M. and Bagowski, Christoph P. (2009). Metastatic behaviour of primary human tumours in a zebrafish xenotransplantation model. BMC Cancer, 9 (Article# 128) 128, 1-13. doi: 10.1186/1471-2407-9-128
2008
Journal Article
Missorting of the Aquaporin-2 mutant E258K to multivesicular bodies/lysosomes in dominant NDI is associated with its monoubiquitination and increased phosphorylation by PKC but is due to the loss of E258
Kamsteeg, Erik-Jan, Savelkoul, Paul J. M., Hendriks, Giel, Konings, Irene B. M., Nivillac, Nicole M. I., Lagendijk, Anne Karine., van der Sluijs, Peter and Deen, Peter M. T. (2008). Missorting of the Aquaporin-2 mutant E258K to multivesicular bodies/lysosomes in dominant NDI is associated with its monoubiquitination and increased phosphorylation by PKC but is due to the loss of E258. Pflugers Archiv European Journal of Physiology, 455 (6), 1041-1054. doi: 10.1007/s00424-007-0364-6
2007
Journal Article
Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development
Kloosterman, Wigard P., Lagendijk, Anne K., Ketting, Rene F., Moulton, Jon D. and Plasterk, Ronal H. A. (2007). Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biology, 5 (8), 1738-1749. doi: 10.1371/journal.pbio.0050203
2006
Journal Article
Polarisation, key to good localisation
van Beest, Moniek, Robben, Joris H., Savelkoul, Paul J. M., Hendriks, Giel, Devonald, Mark A. J., Konings, Irene B. M., Lagendijk, Anne K., Karet, Fiona and Deen, Peter M. T. (2006). Polarisation, key to good localisation. BBA: Biomembranes, 1758 (8), 1126-1133. doi: 10.1016/j.bbamem.2006.03.007
Funding
Current funding
Past funding
Supervision
Availability
- Associate Professor Anne Lagendijk is:
- Available for supervision
Before you email them, read our advice on how to contact a supervisor.
Available projects
-
How to make and maintain a healthy vasculature
Our group aims to understand how our blood vessel network is established during embryonic development and how its function is maintained during life. The development of an aberrant vessel network contributes to a range of cardio-vascular diseases. For example leaky vessels in the brain can cause stroke. In the lab we employ the zebrafish as a model organism since zebrafish embryos are viable ex utero and can be imaged at extremely high resolution which gives us a unique, live, view of vascular development. We make use of existing and novel biosensors in zebrafish to reveal dynamics of adhesion complexes at the cell-cell and cell-matrix interface whilst also examining distribution of tension at these mechanical hubs. By combining these high-end imaging approaches with detailed, innovative molecular genetics approaches, like CRISPR mutagenesis, we explore the fundamental importance of cell-matrix adhesion in vascular biology. We complement this in vivo modelling with analysis of bioengineered human micro-vessels. This adaptable system allows us to detail the impact of physical cues by the environment and by blood flow.
PhD projects include:
- The role of neuropeptides in establishing and maintaining blood vessels in zebrafish and 3D bioengineered human vessels.
- Using CRISPR mutagenesis to uncover novel players that drive a vascular disease that can cause stroke.
- Modelling how the brain vasculature impacts on brain tumors growth and cancer treatment using zebrafish models.
- Profiling changes in tension across wild-type and mutant forms of the adhesion protein VE-cadherin using in vivo live imaging.
- The impact of cell-matrix interactions on vascular growth in zebrafish mutant models.
Supervision history
Current supervision
-
Doctor Philosophy
Uncovering why CCM vascular malformations are restricted to the brain.
Principal Advisor
Other advisors: Professor Robert Parton
-
Doctor Philosophy
Targetting blood vessel dysfunction in disease
Principal Advisor
Other advisors: Dr Samantha Stehbens, Dr Emma Gordon
-
Doctor Philosophy
Investigating the mechanosensory properties of blood vessels
Principal Advisor
Other advisors: Dr Samantha Stehbens, Dr Emma Gordon
-
Doctor Philosophy
Targetting blood vessel dysfunction in disease
Principal Advisor
Other advisors: Dr Samantha Stehbens, Dr Emma Gordon
-
Doctor Philosophy
Investigating the role of the blood vasculature in Medulloblastoma progression using zebrafish models
Principal Advisor
Other advisors: Dr Laura Genovesi, Dr Emma Gordon
-
Doctor Philosophy
Characterisation of a molecular pathway controlling cell-cell adhesion in veins but not arteries
Principal Advisor
Other advisors: Professor Alpha Yap
-
Doctor Philosophy
Characterisation of a molecular pathway controlling cell-cell adhesion in veins but not arteries
Principal Advisor
Other advisors: Professor Alpha Yap
-
Doctor Philosophy
Characterisation of a molecular pathway controlling cell-cell adhesion in veins but not arteries
Principal Advisor
Other advisors: Professor Alpha Yap
-
Doctor Philosophy
Investigating the role of the blood vasculature in Medulloblastoma progression using zebrafish models
Principal Advisor
Other advisors: Dr Laura Genovesi, Dr Emma Gordon
-
Doctor Philosophy
Uncovering dynamic changes in tumour associated vasculature using zebrafish models
Principal Advisor
Other advisors: Dr Laura Genovesi, Dr Emma Gordon
-
Doctor Philosophy
How the interaction between blood flow forces and ECM controls vessel assembly and function during development
Associate Advisor
Other advisors: Dr Mel White
-
Doctor Philosophy
The role of c-Src in facilitating endothelial dysfunction in aortic disease
Associate Advisor
Other advisors: Dr Emma Gordon, Dr Lilian Schimmel
-
Doctor Philosophy
The role of c-Src in facilitating endothelial dysfunction in aortic disease
Associate Advisor
Other advisors: Dr Emma Gordon, Dr Lilian Schimmel
-
Doctor Philosophy
The role of c-Src in facilitating endothelial dysfunction in aortic disease
Associate Advisor
Other advisors: Dr Emma Gordon, Dr Lilian Schimmel
Completed supervision
-
2021
Doctor Philosophy
Cell Polarity and Cell-Matrix Adhesion in Vascular Development
Principal Advisor
-
2025
Doctor Philosophy
The role of c-Src in facilitating endothelial dysfunction in aortic disease
Associate Advisor
Other advisors: Dr Emma Gordon, Dr Lilian Schimmel
-
2020
Doctor Philosophy
Defining the early molecular events governing AVC formation in zebrafish
Associate Advisor
-
2019
Doctor Philosophy
Elimination of apoptotic epithelial cells: Regulation of apoptotic extrusion and immune responses to epithelial apoptosis
Associate Advisor
Other advisors: Professor Alpha Yap
-
2018
Doctor Philosophy
Characterisation of novel regulators of cardiovascular development in zebrafish
Associate Advisor
-
2017
Doctor Philosophy
Characterisation of novel molecular mechanisms of lymphatic vascular development
Associate Advisor
Media
Enquiries
For media enquiries about Associate Professor Anne Lagendijk's areas of expertise, story ideas and help finding experts, contact our Media team: