Overview
Background
Professor Lars Nielsen is leading the development of experimental and computational tools to analyse and design complex biological systems. His expertise in metabolic modelling and flux analysis is available nowhere else in Australia – and in few labs across the world. Professor Nielsen’s studies of biological systems as diverse as bacteria, baker's yeast, sugarcane, insects and mammals has attracted industrial partnerships with companies including Dow, Metabolix, Amyris, LanzaTech, Boeing, Virgin Australia and GE. These metabolic engineering partnerships have focussed on developing new ways of producing aviation fuel, various materials and bioactives (antibiotics, biopesticides, monoclonal antibodies). Professor Nielsen is also applying system analysis and design approaches to tissue engineering including novel strategies for generating microtissues for drug screening and using stem cells to produce red and white blood cells for transfusion.
International links
Professor Nielsen collaborates with some of the world’s pre-eminent metabolic engineers. A joint project with Prof Sang Yup Lee (KAIST, Korea) enabled several extended mutual visits to explore use of sugar for higher value products. A separate project focused on producing synthetic aviation fuel based on isoprenoids involves Professor Nielsen collaborating with global synthetic biotechnology company Amyris and leading isoprenoid metabolic engineer Professor Jay Keasling, from UC Berkeley. Professor Nielsen has secured $8million since 2006 from industry through research grants with US, European, Japanese, Korean, New Zealand and Australian companies.
Availability
- Professor Lars Nielsen is:
- Available for supervision
- Media expert
Fields of research
Qualifications
- Masters (Coursework), Technical University of Denmark
- Doctor of Philosophy, The University of Queensland
Research impacts
Research
Systems and synthetic biology. Professor Nielsen is dedicated to both the advancement of bioengineering science and its application to specific problems. Using thermodynamic principles, novel approaches are developed for handling complex, transient dynamics in developing tissue as well as rational design of complex pathways. These novel approaches are used in the design of bioprocesses as diverse as the production of blood cells for transfusion and the production of industrial biopolymers.
Memberships, patents and funding
Professor Nielsen has been granted four patents – two in stem cells and another two in metabolic engineering. He is on the Scientific Advisory Board of InSphero (2009–), a Swiss company commercialising microtissue technology originating in the Nielsen laboratory. He is an editorial board member of ACS Synthetic Biology, Metabolic Engineering, Biotechnology Journal, Biotechnology and Bioengineering and Bioprocess and Biosystems Engineering. Professor Nielsen formed and chaired the 2005 CRC SIIB Industrial Biotechnology Panel to develop an industrial biotechnology strategy for the Australian sugar industry. He successfully lobbied for the inclusion of Industrial Biotechnology in the Queensland Biotechnology Strategic Plan 2005-2015. Since then he has served as expert advisor to government, research bodies, domestic and major international companies such as Dow Chemical, DuPont and GS Caltex. In the past decade, he has been part of successful research grant applications totalling $56 million, with $31.2 million for his own systems and synthetic biology group at AIBN.
Awards and plenaries
Professor Nielsen has received the UQ Foundation Research Excellence Award; and the Australian Institute of Political Science Queensland Young Tall Poppy Award. He was invited to speak at the 2010 Science at the Shine Dome Symposium on Genomics & Mathematics, Canberra; and the Australian Academy of Technical Sciences & Engineering’s National Symposium on Future-Proofing Australia.
Works
Search Professor Lars Nielsen’s works on UQ eSpace
2005
Journal Article
Emulsion strategies in the microencapsulation of cells: Pathways to thin coherent membranes
Leung, A, Ramaswamy, Y, Munro, P, Lawrie, G, Nielsen, L and Trau, M (2005). Emulsion strategies in the microencapsulation of cells: Pathways to thin coherent membranes. Biotechnology And Bioengineering, 92 (1), 45-53. doi: 10.1002/bit.20597
2005
Journal Article
Modeling hybridoma cell metabolism using a generic genome-scale metabolic model of Mus musculus
Sheikh, K. M., Forster, J. and Nielsen, L. K. (2005). Modeling hybridoma cell metabolism using a generic genome-scale metabolic model of Mus musculus. Biotechnology Progress, 21 (1), 112-121. doi: 10.1021/bp0498138
2005
Journal Article
Properties of a unique mutant of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus that exhibits a partial many polyhedra and few polyhedra phenotype on extended serial passaging in suspension cell cultures
Pedrini, Marcia R. S., Nielsen, Lars K., Reid, Steven and Chan, Leslie C. L. (2005). Properties of a unique mutant of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus that exhibits a partial many polyhedra and few polyhedra phenotype on extended serial passaging in suspension cell cultures. In Vitro Cellular & Developmental Biology - Animal, 41 (8-9), 289-297. doi: 10.1290/0503019R.1
2005
Conference Publication
Development of sugarcane as a biofactory for biopolymers
Brumbley, S. M., Purnell, M. P., Petrasovits, L. A., McQualter, R., Fong Chong, B., Meyer, K., Van Dyk, D. E., O'Shea, M. G., Walton, N. J., Nielsen, L. K. and Viitanen, P. V. (2005). Development of sugarcane as a biofactory for biopolymers. The Second World Congress on Industrial Biotechnology and Bioprocessing: Linking Biotechnology, Chemistry and Agriculture to Create New Value Chains, Orlando, FL, USA, 20–22 April 2005.
2004
Journal Article
Identification of three gene candidates for multicellular resistance in colon carcinoma
Timmins, N. E., Maguire, T. L., Grimmond, S. M. and Nielsen, L. K. (2004). Identification of three gene candidates for multicellular resistance in colon carcinoma. Cytotechnology, 46 (1), 9-18. doi: 10.1007/s10616-005-1476-5
2004
Journal Article
Hanging-drop multicellular spheriods as a model of tumour angiogenesis
Timmins, N. E., Dietmair, S. and Nielsen, L. K. (2004). Hanging-drop multicellular spheriods as a model of tumour angiogenesis. Angiogenesis, 7 (2), 97-103. doi: 10.1007/s10456-004-8911-7
2004
Conference Publication
Development Of sugarcane as a biofactory for biopolymers
Brumbley, S. M., Purnell, M. P., Petrasovits, L. A., McQualter, R., Fong Chong, B., Meyer, K., Van Dyk, D. E. and Nielsen, L. K. (2004). Development Of sugarcane as a biofactory for biopolymers. International Symposium on Biological Polyesters (ISBP 2004), Beijing, China, 22-28 August 2004.
2004
Journal Article
Design of artificial myocardial microtissues
Kelm, J. M., Ehler, E., Nielsen, L. K., Schlatter, S., Perriard, J. C. and Fussenegger, M. (2004). Design of artificial myocardial microtissues. Tissue Engineering, 10 (1-2), 201-214. doi: 10.1089/107632704322791853
2004
Conference Publication
Development of sugarcane as a biofactory for biopolymers
Brumbley, Stevens M., Purnell, Matt P., Petrasovits, Lars A., O'Shea, Michael G. and Nielsen, Lars K. (2004). Development of sugarcane as a biofactory for biopolymers. Plant & Animal Genomes XII Conference, San Diego, U.S., 10-14 January 2004. San Deigo, California, U.S.: GenomeWeb.
2003
Journal Article
Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types
Kelm, Jens M., Timmins, Nicholas E., Brown, Catherine J., Fussenegger, Martin and Nielsen, Lars K. (2003). Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types. Biotechnology And Bioengineering, 83 (2), 173-180. doi: 10.1002/bit.10655
2003
Conference Publication
Production of polyhydroxyalkanoates in sugarcane
Brumbley, S. M., Petrasovits, L., Purnell, M., O'Shea, M. and Nielsen, L (2003). Production of polyhydroxyalkanoates in sugarcane. 6th Asia Pacific Biochemical Engineering Conference (APBioChEC): Harnessing New Technology for Bioprocess Development, Brisbane, Australia, 30 November- 4 December 2003.
2003
Journal Article
Sensitivity of Helicoverpa armigera nucleopolyhedrovirus polyhedra to sodium dodecyl sulfate
Lua, L. H. L., Nielsen, L. K. and Reid, S. (2003). Sensitivity of Helicoverpa armigera nucleopolyhedrovirus polyhedra to sodium dodecyl sulfate. Biological Control, 26 (1), 57-67. doi: 10.1016/S1049-9644(02)00116-0
2003
Conference Publication
Production of polyhydroxyalkanoates in sugarcane
Brumbley, S. M., Petrasovits, L. A., Bonaventura, P. A., O'Shea, M. J., Purnell, M. P. and Nielsen, L. K. (2003). Production of polyhydroxyalkanoates in sugarcane. 4th ISSCT Molecular Biology Workshop, Montpelier, France, 7-14 April, 2003.
2003
Journal Article
Amplifying the cellular reduction potential of Streptococcus zooepidemicus
Chong, B. F. and Nielsen, L. K. (2003). Amplifying the cellular reduction potential of Streptococcus zooepidemicus. Journal of Biotechnology, 100 (1), 33-41. doi: 10.1016/S0168-1656(02)00239-0
2003
Conference Publication
Production of polyhydroxyalkanoates in sugarcane
Brumbley, S. M., Petrasovits, L. A., Bonaventura, P. A., O'Shea, M. J., Purnell, M. P. and Nielsen, L. K. (2003). Production of polyhydroxyalkanoates in sugarcane. IVth International Workshop on Plant Biotechnology and Sustainable Development (BioVeg2003), Ciego de Avila, Cuba, 14-19 April 2003.
2003
Journal Article
Aerobic cultivation of Streptococcus zooepidemicus and the role of NADH oxidase
Fong Chong, B. and Nielsen, L. K. (2003). Aerobic cultivation of Streptococcus zooepidemicus and the role of NADH oxidase. Biochemical Engineering Journal, 16 (2), 153-162. doi: 10.1016/S1369-703X(03)00031-7
2003
Journal Article
Quantitative prediction of phase diagrams for polymer partitioning in aqueous two-phase systems
Croll, T., Munro, P. D., Winzor, D. J., Trau, M. and Nielsen, L. K. (2003). Quantitative prediction of phase diagrams for polymer partitioning in aqueous two-phase systems. Journal of Polymer Science Part B-polymer Physics, 41 (5), 437-443. doi: 10.1002/polb.10402
2003
Journal Article
Analysis of the phase behavior of the aqueous poly(ethylene glycol)-Ficoll system
Croll, T., Munro, P., Winzor, D. J., Trau, M. and Nielsen, L. K. (2003). Analysis of the phase behavior of the aqueous poly(ethylene glycol)-Ficoll system. Biotechnology Progress, 19 (4), 1269-1273. doi: 10.1021/bp025711a
2002
Journal Article
Kinetics of baculovirus replication and release using real-time quantitative polymerase chain reaction
Rosinski, Matthew, Reid, Steven and Nielsen, Lars Keld (2002). Kinetics of baculovirus replication and release using real-time quantitative polymerase chain reaction. Biotechnology And Bioengineering, 77 (4), 476-480. doi: 10.1002/bit.10126
2002
Conference Publication
Application of biotechnology for future sugar industry diversification
Brumbley, S. M., Petrasovits, L., Purnell, M., O'Shea, M. G., Geijskes, J., Prakash Lakshmanan, Smith, G. R. and Nielsen, L. K. (2002). Application of biotechnology for future sugar industry diversification. 2002 Conference of the Australian Society of Sugar Cane Technologists, Cairns, Queensland, Australia, 29 April - 2 May, 2002. PK Editorial Services Pty Ltd.
Funding
Current funding
Past funding
Supervision
Availability
- Professor Lars Nielsen is:
- Available for supervision
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Supervision history
Current supervision
-
Doctor Philosophy
C1 Global Metabolic Atlas
Principal Advisor
-
Doctor Philosophy
Biological CO2 and H2 utilization for sustainable animal feed production
Associate Advisor
Other advisors: Dr James Heffernan, Professor Esteban Marcellin
-
Doctor Philosophy
Biorecycyling carbon using acetogens
Associate Advisor
Other advisors: Professor Gary Schenk, Dr Axa Gonzalez, Dr James Heffernan, Professor Esteban Marcellin
Completed supervision
-
2021
Doctor Philosophy
Investigating High Dimensional Alternative Splicing During Haematopoiesis.
Principal Advisor
Other advisors: Dr Darren Korbie
-
2017
Doctor Philosophy
A Bayesian framework for sampling the feasible kinetic behavior of metabolic reaction networks
Principal Advisor
-
2016
Doctor Philosophy
Understanding CHO cells biology for enhanced biopharmaceutical production: a comparative transcriptomic and proteomic approach
Principal Advisor
Other advisors: Professor Esteban Marcellin, Professor Peter Gray
-
2016
Doctor Philosophy
Order from Noise: Modelling the Stochasticity of Lineage Commitment
Principal Advisor
-
2016
Doctor Philosophy
Unraveling metabolic flux control of the methylerythritol phosphate (MEP) pathway: a systems biology study
Principal Advisor
-
2015
Doctor Philosophy
RNA CaptureSeq: Targeted Sequencing for Comprehensive Transcriptome Studies
Principal Advisor
Other advisors: Professor Timothy Mercer
-
2015
Doctor Philosophy
Systems Level Signalling Analysis A Case Study of Growth Hormone Receptor Signalling
Principal Advisor
-
2015
Doctor Philosophy
Quantitative bacterial phosphoproteomics
Principal Advisor
Other advisors: Professor Esteban Marcellin
-
2014
Doctor Philosophy
Understanding and overcoming monoterpene toxicity in Saccharomyces cerevisia for the production of sustainable jet fuels
Principal Advisor
Other advisors: Professor Peter Gray
-
2014
Doctor Philosophy
Global metabolic effect of manipulating pyruvate dehydrogenase complex activity in mammalian cells
Principal Advisor
Other advisors: Professor Peter Gray
-
2014
Doctor Philosophy
Efficient production of a model designed peptide from sucrose in Escherichia coli
Principal Advisor
-
2014
Doctor Philosophy
Simulating Information Flow in Cellular Scale Models of Signal Transduction
Principal Advisor
-
2014
Doctor Philosophy
Characterisation of the Saccharopolyspora erythraea metabolic switch: a systems approach
Principal Advisor
Other advisors: Professor Esteban Marcellin
-
2014
Doctor Philosophy
Using network thermodynamics and metabolomics for the study of dynamic mammalian cell metabolism
Principal Advisor
Other advisors: Professor Peter Gray
-
2013
Doctor Philosophy
Understanding phenotypic asymmetry in Escherichia coli: Development and characterization of multi-omics datasets to explain diversity amongst strains of E. coli
Principal Advisor
-
2012
Doctor Philosophy
Characterisation of mammalian cells during protein production - a systems biology approach
Principal Advisor
Other advisors: Professor Peter Gray
-
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2011
Doctor Philosophy
Carbon Mobilisation and Utilisation in the Sugarcane Biofactory
Principal Advisor
-
-
2010
Doctor Philosophy
Understanding molecular weight control of hyaluronic acid production in Streptococcus zooepidemicus: towards a systems approach
Principal Advisor
-
2010
Doctor Philosophy
System level assessment of mammalian cell metabolim for production of recombinant protein
Principal Advisor
-
2009
Doctor Philosophy
Directed differentiation of mouse embryonic stem cells to haematopoietic lineages using EPL induction
Principal Advisor
-
2008
Doctor Philosophy
Self Assembled Nano-coats for Protection of Foreign Tissue Transplants
Principal Advisor
Other advisors: Professor Matt Trau
-
2006
Doctor Philosophy
HYALURONIC ACID PRODUCTION IN CONTINUOUS CULTURES OF STREPTOCOCCUS ZOOEPIDEMICUS
Principal Advisor
-
2005
Doctor Philosophy
OPTIMIZATION OF DENDRITIC CELLS FOR CANCER IMMUNOTHERAPY
Principal Advisor
Other advisors: Professor Ranjeny Thomas
-
2005
Doctor Philosophy
PRODUCTION OF POLY-HYDROXYALKANOATES IN SUGARCANE
Principal Advisor
-
2005
Doctor Philosophy
METABOLIC FLUX ANALYSIS OF EUKARYOTIC CELLS: A STUDY OF THEORETICAL AND EXPERIMENTAL FRAMEWORKS
Principal Advisor
-
2004
Doctor Philosophy
QUANTITATIVE ELUCIDATION OF THE PHENOMENOLOGICAL SELECTIVE ADVANTAGE OF THE FEW POLYHEDRA MUTANT STRAIN OF Helicoverpa armigera NUCLEOPOLYHEDROVIRUS UNDER in-vitro CONDITIONS
Principal Advisor
-
2004
Doctor Philosophy
EXTENDING THE THIRD DIMENSION: NOVEL METHODS AND APPLICATIONS FOR 3D MULTICELLULAR SPHEROIDS
Principal Advisor
-
2002
Doctor Philosophy
METABOLIC ENGINEERING OF HYALURONIC ACID PRODUCTION
Principal Advisor
-
2002
Doctor Philosophy
HYALURONIC ACID OVERPRODUCTION BY METABOLIC PATHWAY MANIPULATION
Principal Advisor
-
2023
Doctor Philosophy
Modelling and characterising life and death of mammalian suspension cultures
Associate Advisor
Other advisors: Dr Verónica Martinez Salazar, Dr Gert Talbo, Professor Esteban Marcellin
-
2023
Doctor Philosophy
Sustainable fuel and chemical production via Gas Fermentation: exploring CO2 metabolism by Clostridium autoethanogenum
Associate Advisor
Other advisors: Dr Axa Gonzalez, Professor Esteban Marcellin
-
2021
Doctor Philosophy
Insight into acetogens metabolism through the use of thermodynamic metabolic flux analysis
Associate Advisor
Other advisors: Professor Esteban Marcellin
-
2020
Doctor Philosophy
Understanding toxin production in Clostridium tetani: a systems approach
Associate Advisor
Other advisors: Professor Esteban Marcellin
-
2019
Doctor Philosophy
From waste to chemicals: On the metabolism of Clostridium autoethanogenum and PHB production from gas fermentation
Associate Advisor
Other advisors: Professor Esteban Marcellin
-
2018
Doctor Philosophy
Exploring the metabolism of propionibacteria using genome-scale modelling
Associate Advisor
Other advisors: Professor Esteban Marcellin
-
2018
Doctor Philosophy
Bioeroding sponges in a time of change: insights into the physiology and cell biology of a photosymbiotic coral-eroding sponge
Associate Advisor
Other advisors: Professor Ove Hoegh-Guldberg
-
2018
Doctor Philosophy
Modulating gene expression in yeast to optimize metabolic networks for sesquiterpene and monoterpene production
Associate Advisor
-
2018
Doctor Philosophy
Metabolic Engineering of propionibacteria's Wood Werkman cycle in Escherichia coli and Saccharomyces cerevisiae
Associate Advisor
Other advisors: Professor Mark Turner, Professor Esteban Marcellin
-
2017
Doctor Philosophy
Understanding high propionic acid production in Propionibacterium
Associate Advisor
Other advisors: Professor Esteban Marcellin
-
2015
Doctor Philosophy
Novel Cell Engineering of the Unfolded Protein Response to Achieve Efficient Therapeutic Protein Production Cell Line
Associate Advisor
Other advisors: Professor Peter Gray
-
2015
Doctor Philosophy
Separating Growth and Production Phases Using Synthetic Quorum Sensing in Saccharomyces cerevisiae
Associate Advisor
-
2013
Doctor Philosophy
Metabolic engineering for bio-production of industrial isoprenoids from sucrose using Escherichia coli
Associate Advisor
-
2010
Doctor Philosophy
A microfluidic platform for screening immobilised biomolecule mixtures
Associate Advisor
Other advisors: Professor Justin Cooper-White
-
2008
Doctor Philosophy
Angular Momentum in Optical Tweezers
Associate Advisor
Other advisors: Professor Halina Rubinsztein-Dunlop, Dr Timo Nieminen
-
2003
Doctor Philosophy
STABILITY STUDIES OF WILD TYPE BACULOVIRUSES PRODUCED VIA FERMENTATION - HELIOTHIS AS A MODEL SYSTEM
Associate Advisor
Media
Enquiries
Contact Professor Lars Nielsen directly for media enquiries about:
- Engineering - biological
- Metabolic engineering
- Synthetic biology
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