Overview
Background
1991-94 BSc Honours I (Chemistry) University of Queensland, University Medal
1995-99 PhD (Cancer Pathology) University of Queensland
1999-00 Post-Doctoral Fellow, Queensland University of Technology
2001-03 NHMRC CJ Martin/RG Menzies Fellow, Scripps Research Institute, San Diego, CA, USA
2003-05 NHMRC CJ Martin/RG Menzies Fellow, Queensland University of Technology
2005-09 NHMRC RD Wright Fellow, Queensland University of Technology
2010-15 Associate Professor, Mater Research Institute, The University of Queensland
2012-16 ARC Future Fellow, Mater Research Institute, The University of Queensland
2016- Professor of Cancer Biology, Mater Research Institute, The University of Queensland
Availability
- Honorary Professor John Hooper is:
- Available for supervision
Research interests
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Cancers of the reproductive system and gastrointestinal tract
Our focus is on the identification and evaluation of molecular targets and biomarkers of cancer. As much as possible our research employs disease relevant models that incorporate patient tumours. We have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals.
Research impacts
My major research contributions are in the identification and evaluation of molecular targets and biomarkers for cancers of the ovary, pancreas, prostate and bowel. At a molecular level my focus is on cell surface receptors, proteolytic enzymes, intracellular signal transducers, mediators of metabolism and protein post-translational modifications. Most recently we have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals. To date my research has attracted ~$11M in funding, producing 4 patents and 95 papers.
Works
Search Professor John Hooper’s works on UQ eSpace
2018
Journal Article
The potential role of miRNAs and exosomes in chemotherapy in ovarian cancer
Alharbi, Mona, Zuñiga, Felipe, Elfeky, Omar, Guanzon, Dominic, Lai, Andrew, Rice, Gregory E., Perrin, Lewis, Hooper, John and Salomon, Carlos (2018). The potential role of miRNAs and exosomes in chemotherapy in ovarian cancer. Endocrine-Related Cancer, 25 (12), R663-R685. doi: 10.1530/erc-18-0019
2018
Journal Article
Proteomic analysis of exosomes reveals an association between cell invasiveness and exosomal bioactivity on endothelial and mesenchymal cell migration
Sharma, Shayna, Alharbi, Mona, Kobayashi, Miharu, Lai, Andrew, Guanzon, Dominic, Zuñiga, Felipe, Ormazabal, Valeska, Palma, Carlos, Scholz-Romero, Katherin, Rice, Gregory E., Hooper, John D. and Salomon, Carlos (2018). Proteomic analysis of exosomes reveals an association between cell invasiveness and exosomal bioactivity on endothelial and mesenchymal cell migration. Clinical Science, 132 (18), 2029-2044. doi: 10.1042/CS20180425
2018
Journal Article
Evidence that cell surface localization of serine protease activity facilitates cleavage of the protease activated receptor CDCP1
He, Yaowu, Reid, Janet C., He, Hui, Harrington, Brittney S., Finlayson, Brittney, Khan, Tashbib and Hooper, John D. (2018). Evidence that cell surface localization of serine protease activity facilitates cleavage of the protease activated receptor CDCP1. Biological Chemistry, 399 (9), 1091-1097. doi: 10.1515/hsz-2017-0308
2018
Journal Article
L1 retrotransposon heterogeneity in ovarian tumor cell evolution
Nguyen, Thu H. M., Carreira, Patricia E., Sanchez-Luque, Francisco J., Schauer, Stephanie N., Fagg, Allister C., Richardson, Sandra R., Davies, Claire M., Jesuadian, J. Samuel, Kempen, Marie-Jeanne H. C., Troskie, Robin-Lee, James, Cini, Beaven, Elizabeth A., Wallis, Tristan P., Coward, Jermaine I. G., Chetty, Naven P., Crandon, Alexander J., Venter, Deon J., Armes, Jane E., Perrin, Lewis C., Hooper, John D., Ewing, Adam D., Upton, Kyle R. and Faulkner, Geoffrey J. (2018). L1 retrotransposon heterogeneity in ovarian tumor cell evolution. Cell Reports, 23 (13), 3730-3740. doi: 10.1016/j.celrep.2018.05.090
2018
Conference Publication
Granulocyte Colony Stimulating Factor impacts on osteomacs and bone marrow macrophages - implications for prostate cancer osteoblastic lesion formation
Millard, Susan, Wu, Andy, Kaur, Simran, He, Yaowu, Batoon, Lena, Hooper, John and Pettit, Allison (2018). Granulocyte Colony Stimulating Factor impacts on osteomacs and bone marrow macrophages - implications for prostate cancer osteoblastic lesion formation. Annual Meeting of the American Society for Bone and Mineral Research, Montréal, Quebéc, Canada, Sep 28-Oct 01, 2018. Hoboken, NJ, United States: Wiley-Blackwell Publishing. doi: 10.1002/jbmr.3621
2018
Conference Publication
Integrative Analysis of Multi-Omics Exosomal Data for Identifying Multi-Markers for Susceptibility to Chemotherapy in Ovarian Cancer
Alharbi, Mona, Lai, Andrew, Guanzon, Dominic, Sharma, Shayna, Palma, Carlos, Zuniga, Felipe, Rice, Gregory E., Perrin, Lewis, Hooper, John D. and Salomon, Carlos (2018). Integrative Analysis of Multi-Omics Exosomal Data for Identifying Multi-Markers for Susceptibility to Chemotherapy in Ovarian Cancer. 65th Annual Scientific Meeting of the Society-for-Reproductive-Investigation (SRI), San Diego Ca United States, 6-10 March 2018. Thousand Oaks, CA United States: Sage.
2017
Journal Article
Tumor-derived exosomes in ovarian cancer - liquid biopsies for early detection and real-time monitoring of cancer progression
Sharma, Shayna, Zuñiga, Felipe, Rice, Gregory E., Perrin, Lewis C., Hooper, John D. and Salomon, Carlos (2017). Tumor-derived exosomes in ovarian cancer - liquid biopsies for early detection and real-time monitoring of cancer progression. Oncotarget, 8 (61), 104687-104703. doi: 10.18632/oncotarget.22191
2017
Journal Article
Kallikrein-related peptidase 4 induces cancer-associated fibroblast features in prostate-derived stromal cells
Kryza, Thomas, Silva, Lakmali M., Bock, Nathalie, Fuhrman-Luck, Ruth, Stephens, Carson, Gao, Jin, Samaratunga, Hema, Lawrence, Mitchell, Hooper, John D., Dong, Ying, Risbridger, Gail and Clements, Judith A. (2017). Kallikrein-related peptidase 4 induces cancer-associated fibroblast features in prostate-derived stromal cells. Molecular Oncology, 11 (10), 1307-1329. doi: 10.1002/1878-0261.12075
2017
Journal Article
The emerging roles of extracellular vesicles as communication vehicles within the tumor microenvironment and beyond
Sullivan, Ryan, Maresh, Grace, Zhang, Xin, Salomon, Carlos, Hooper, John, Margolin, David and Li, Li (2017). The emerging roles of extracellular vesicles as communication vehicles within the tumor microenvironment and beyond. Frontiers in Endocrinology, 8 (AUG) 194, 194. doi: 10.3389/fendo.2017.00194
2017
Journal Article
Plasmin(ogen) serves as a favorable biomarker for prediction of survival in advanced high-grade serous ovarian cancer
Zhao, Shuo, Dorn, Julia, Napieralski, Rudolf, Walch, Axel, Diersch, Sandra, Kotzsch, Matthias, Ahmed, Nancy, Hooper, John D., Kiechle, Marion, Schmitt, Manfred and Magdolen, Viktor (2017). Plasmin(ogen) serves as a favorable biomarker for prediction of survival in advanced high-grade serous ovarian cancer. Biological Chemistry, 398 (7), 765-773. doi: 10.1515/hsz-2016-0282
2017
Journal Article
Expression of CDCA3 is a prognostic biomarker and potential therapeutic target in non–small cell lung cancer
Adams, Mark N., Burgess, Joshua T., He, Yaowu, Gately, Kathy, Snell, Cameron, Zhang, Shu-Dong, Hooper, John D., Richard, Derek J. and O'Byrne, Kenneth J. (2017). Expression of CDCA3 is a prognostic biomarker and potential therapeutic target in non–small cell lung cancer. Journal of Thoracic Oncology, 12 (7), 1071-1084. doi: 10.1016/j.jtho.2017.04.018
2017
Journal Article
Development of an enzyme-linked immunosorbent assay for detection of CDCP1 shed from the cell surface and present in colorectal cancer serum specimens
Chen, Yang, Harrington, Brittney S., Lau, Kevin C. N., Burke, Lez J., He, Yaowu, Iconomou, Mary, Palmer, James S., Meade, Brian, Lumley, John W. and Hooper, John D. (2017). Development of an enzyme-linked immunosorbent assay for detection of CDCP1 shed from the cell surface and present in colorectal cancer serum specimens. Journal of Pharmaceutical and Biomedical Analysis, 139, 65-72. doi: 10.1016/j.jpba.2017.02.047
2017
Conference Publication
Exosomal content in the plasma of patients with ovarian cancer reflect tumor state and induce the epithelial to mesenchymal transition in target cells
Sharma, Shayna, Scholz-Romero, Katherin, Kline, Richard, Wade, Katrina, Estes, Jacob, Palma, Carlos, Guanzon, Dominic, Lai, Andrew, Hooper, John, Rice, Gregory E. and Salomon, Carlos (2017). Exosomal content in the plasma of patients with ovarian cancer reflect tumor state and induce the epithelial to mesenchymal transition in target cells. 64th Annual Scientific Meeting of the Society for Reproductive Investigation (SRI), Orlando, FL, United States, 15-18 March 2017. Thousand Oaks, CA, United States: Sage Publications. doi: 10.1177/1933719117699773
2017
Journal Article
Mapping transmembrane residues of proteinase activated recpetor 2 (PAR2) that influence ligand-modulated calcium signaling
Suen, J.Y., Adams, M. N., Lim, J., Madala, P.K., Xu, W, Cotterell, A., He, Y., Yua, Mei-Kwan, Hooper, J. D. and Fairlie, D.P. (2017). Mapping transmembrane residues of proteinase activated recpetor 2 (PAR2) that influence ligand-modulated calcium signaling. Pharmacological Research, 117, 328-342. doi: 10.1016/j.phrs.2016.12.020
2017
Journal Article
MUC13 overexpression in renal cell carcinoma plays a central role in tumor progression and drug resistance
Sheng, Yonghua, Ng, Choa Ping, Lourie, Rohan, Shah, Esha T., He, Yaowu, Wong, Kuan Yua, Seim, Inge, Oancea, Iulia, Morais, Christudas, Jeffery, Penny L., Hooper, John, Gobe, Glenda C. and Mcguckin, Michael A. (2017). MUC13 overexpression in renal cell carcinoma plays a central role in tumor progression and drug resistance. International Journal of Cancer, 140 (10), 2351-2363. doi: 10.1002/ijc.30651
2017
Conference Publication
Ovarian cancer cells transfer resistance to chemotherapy to other cells via exosomes
Alharbi, Mona, Kline, Richard, Wade, Katrina, Estes, Jacob, Hooper, John, Rice, Gregory E. and Salomon, Carlos (2017). Ovarian cancer cells transfer resistance to chemotherapy to other cells via exosomes. 64th Annual Scientific Meeting of the Society for Reproductive Investigation (SRI), Orlando, FL, United States, 5-18 March 2017. Heidelberg, Germany: Springer.
2017
Journal Article
Osteoporosis-Related Health Behaviors in Men With Prostate Cancer and Survivors Exploring Osteoporosis Knowledge, Health Beliefs, and Self-Efficacy
Lassemillante, Annie-Claude M., Skinner, Tina L., Hooper, John D., Prins, John B. and Wright, Olivia R. L. (2017). Osteoporosis-Related Health Behaviors in Men With Prostate Cancer and Survivors Exploring Osteoporosis Knowledge, Health Beliefs, and Self-Efficacy. American Journal of Men’s Health, 11 (1), 13-23. doi: 10.1177/1557988315615956
2017
Journal Article
Pericellular regulation of prostate cancer expressed kallikrein-related peptidases and matrix metalloproteinases by cell surface serine proteases
Reid, Janet C., Matsika, Admire, Davies, Claire M., He, Yaowu, Broomfield, Amy, Bennett, Nigel C., Magdolen, Viktor, Srinivasan, Bhuvana, Clements, Judith A. and Hooper, John D. (2017). Pericellular regulation of prostate cancer expressed kallikrein-related peptidases and matrix metalloproteinases by cell surface serine proteases. American Journal of Cancer Research, 7 (11), 2257-2274.
2016
Journal Article
In vitro evidence that KLK14 regulates the components of the HGF/Met axis, pro-HGF and HGF-activator inhibitor 1A and 1B
Reid, Janet C., Bennett, Nigel C., Stephens, Carson R., Carroll, Melanie L., Magdolen, Viktor, Clements, Judith A. and Hooper, John D. (2016). In vitro evidence that KLK14 regulates the components of the HGF/Met axis, pro-HGF and HGF-activator inhibitor 1A and 1B. Biological Chemistry, 397 (12), 1299-1305. doi: 10.1515/hsz-2016-0163
2016
Journal Article
MUC13 protects colorectal cancer cells from death by activating the NF-κB pathway and is a potential therapeutic target
Sheng, Y. H., He, Y., Hasnain, S. Z., Wang, R., Tong, H., Clarke, D. T., Lourie, R., Oancea, I., Wong, K. Y., Lumley, J. W., Florin, T. H., Sutton, P., Hooper, J. D., McMillan, N. A. and McGuckin, M. A. (2016). MUC13 protects colorectal cancer cells from death by activating the NF-κB pathway and is a potential therapeutic target. Oncogene, 36 (5), 700-713. doi: 10.1038/onc.2016.241
Funding
Current funding
Supervision
Availability
- Honorary Professor John Hooper is:
- Available for supervision
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Supervision history
Current supervision
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Doctor Philosophy
Novel theranostic targets for metastatic colorectal cancer
Principal Advisor
Other advisors: Professor David Clark
-
Doctor Philosophy
Cancer-associated post-translational modifications of the receptor CDCP1 Background:
Principal Advisor
-
Doctor Philosophy
Factors impacting receptor processing in response to peptide and antibody ligands
Principal Advisor
Other advisors: Dr Jodi Saunus
-
Doctor Philosophy
Molecular and cellular determinants of CDCP1 targeted, payload-delivery antibodies.
Principal Advisor
Other advisors: Associate Professor Michael Landsberg
-
Doctor Philosophy
Understanding the function of CDCP1 and its potential as a theranostic target for cholangiocarcinoma
Principal Advisor
Other advisors: Professor Kristofer Thurecht
-
Doctor Philosophy
Investigations into genetic aberrations underlying recurrent ovarian cancer
Principal Advisor
Other advisors: Professor Geoff Faulkner
-
Doctor Philosophy
Developing novel strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
-
Doctor Philosophy
Enhancing immune responses to cancer
Associate Advisor
Other advisors: Dr Jazmina Gonzalez Cruz, Professor Brian Gabrielli
-
Doctor Philosophy
Biomarker driven diagnostic and therapeutic innovations in breast cancer
Associate Advisor
-
Doctor Philosophy
Genomic and epigenomic correlates of prostate cancer therapy
Associate Advisor
Other advisors: Dr Adam Ewing
-
Doctor Philosophy
Characterisation of EV-associated lipids in the progression of ovarian cancer
Associate Advisor
Other advisors: Dr Dominic Guanzon, Professor Carlos Salomon Gallo, Dr Andrew Lai
-
Doctor Philosophy
Developing new strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
Completed supervision
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2022
Doctor Philosophy
Theranostics: Molecular Imaging and Molecularly-Directed Radionuclide Therapy for Metastatic Colorectal Cancer
Principal Advisor
-
2022
Doctor Philosophy
Profiling of Immunoglobulin (Ig) G, IgM and IgA Isotype Immune Responses and Development of Autoantibody Biomarkers for Early Detection of Colorectal Cancer
Principal Advisor
-
2022
Doctor Philosophy
Investigating New Therapeutic Targets for Clear Cell Cancers
Principal Advisor
-
2016
Doctor Philosophy
Thesis Title: Investigation into the role of the cell surface glycoprotein CDCP1 in high-grade serous ovarian cancer progression
Principal Advisor
Other advisors: Professor Brian Gabrielli
-
2020
Doctor Philosophy
Opportunities for epigenetic therapies in ovarian cancer
Associate Advisor
Other advisors: Associate Professor Jason Lee
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2020
Doctor Philosophy
Regulation of Epithelial Ovarian Cancer Initiation and Progression by Exosomal Proteins and miRNAs
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2020
Doctor Philosophy
New Strategies for Identification of Therapeutic Target of Ovarian Cancer
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2018
Master Philosophy
The effects of Rab13 derived from lymph node stromal cell extracellular vesicles on the pathogenesis of colorectal cancer
Associate Advisor
-
2018
Doctor Philosophy
Dysregulation of EGF-trafficking in squamous cell carcinoma and cetuximab resistance
Associate Advisor
Other advisors: Associate Professor Fiona Simpson
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2018
Doctor Philosophy
Targeting Apoptosis as A Novel Therapy for Medulloblastoma
Associate Advisor
Other advisors: Dr Laura Genovesi, Professor Brandon Wainwright
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2010
Doctor Philosophy
Androgen receptor, caveolin-1 and androgen self-sufficiency in prostate cancer
Associate Advisor
Other advisors: Professor David Johnson
Media
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