Overview
Background
1991-94 BSc Honours I (Chemistry) University of Queensland, University Medal
1995-99 PhD (Cancer Pathology) University of Queensland
1999-00 Post-Doctoral Fellow, Queensland University of Technology
2001-03 NHMRC CJ Martin/RG Menzies Fellow, Scripps Research Institute, San Diego, CA, USA
2003-05 NHMRC CJ Martin/RG Menzies Fellow, Queensland University of Technology
2005-09 NHMRC RD Wright Fellow, Queensland University of Technology
2010-15 Associate Professor, Mater Research Institute, The University of Queensland
2012-16 ARC Future Fellow, Mater Research Institute, The University of Queensland
2016- Professor of Cancer Biology, Mater Research Institute, The University of Queensland
Availability
- Honorary Professor John Hooper is:
- Available for supervision
Research interests
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Cancers of the reproductive system and gastrointestinal tract
Our focus is on the identification and evaluation of molecular targets and biomarkers of cancer. As much as possible our research employs disease relevant models that incorporate patient tumours. We have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals.
Research impacts
My major research contributions are in the identification and evaluation of molecular targets and biomarkers for cancers of the ovary, pancreas, prostate and bowel. At a molecular level my focus is on cell surface receptors, proteolytic enzymes, intracellular signal transducers, mediators of metabolism and protein post-translational modifications. Most recently we have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals. To date my research has attracted ~$11M in funding, producing 4 patents and 95 papers.
Works
Search Professor John Hooper’s works on UQ eSpace
2020
Other Outputs
Substrate-biased activity-based probes identify the urokinase-plasminogen axis as a master regulator of metastatic signaling by orphan membrane receptor CDCP1
Kryza, Thomas, Khan, Tashbib, Lovell, Scott, Harrington, Brittney S., Yin, Julia, Porazinski, Sean, Pajic, Marina, Koistinen, Hannu, Rantala, Juha, Dreyer, Tobias, Magdolen, Viktor, Reuning, Ute, He, Yaowu, Tate, Edward and Hooper, John David (2020). Substrate-biased activity-based probes identify the urokinase-plasminogen axis as a master regulator of metastatic signaling by orphan membrane receptor CDCP1.
2020
Other Outputs
Binding proteins to CUB domain-containing protein (CDCP1)
Puttick, S., Kryza, T., Hooper, J. D., He, Y., Harrington, B., Quigley, J. and Deryugina, E. (2020). Binding proteins to CUB domain-containing protein (CDCP1). PCT/AU2020/051216.
2020
Journal Article
PET imaging quantifying Ga-PSMA-11 uptake in metastatic colorectal cancer
Cuda, Tahleesa J., Riddell, Andrew D., Liu, Cheng, Whitehall, Vicki L., Borowsky, Jennifer, Wyld, David K., Burge, Matthew E., Ahern, Elizabeth, Griffin, Alison, Lyons, Nicholas J. R., Rose, Stephen E., Clark, David A., Stevenson, Andrew R. L., Hooper, John D., Puttick, Simon and Thomas, Paul A. (2020). PET imaging quantifying Ga-PSMA-11 uptake in metastatic colorectal cancer. The Journal of Nuclear Medicine, 61 (11), 1576-1579. doi: 10.2967/jnumed.119.233312
2020
Journal Article
Revisiting Glycogen in Cancer: A Conspicuous and Targetable Enabler of Malignant Transformation
Khan, Tashbib, Sullivan, Mitchell A., Gunter, Jennifer H., Kryza, Thomas, Lyons, Nicholas, He, Yaowu and Hooper, John D. (2020). Revisiting Glycogen in Cancer: A Conspicuous and Targetable Enabler of Malignant Transformation. Frontiers in Oncology, 10 592455, 592455. doi: 10.3389/fonc.2020.592455
2020
Journal Article
HBV induced hepatocellular carcinoma and related potential immunotherapy
Jia, Liyang, Gao, Yanan, He, Yaowu, Hooper, John D. and Yang, Pengyuan (2020). HBV induced hepatocellular carcinoma and related potential immunotherapy. Pharmacological Research, 159 104992, 104992. doi: 10.1016/j.phrs.2020.104992
2020
Journal Article
miRNa signature in small extracellular vesicles and their association with platinum resistance and cancer recurrence in ovarian cancer
Alharbi, Mona, Sharma, Shayna, Guanzon, Dominic, Lai, Andrew, Zuñiga, Felipe, Shiddiky, Muhammad J.A., Yamauchi, Yusuke, Salas-Burgos, Alexis, He, Yaowu, Pejovic, Tanja, Winters, Carmen, Morgan, Terry, Perrin, Lewis, Hooper, John D. and Salomon, Carlos (2020). miRNa signature in small extracellular vesicles and their association with platinum resistance and cancer recurrence in ovarian cancer. Nanomedicine: Nanotechnology, Biology, and Medicine, 28 102207, 102207. doi: 10.1016/j.nano.2020.102207
2020
Journal Article
Disruption of glycogen utilization markedly improves the efficacy of carboplatin against preclinical models of clear cell ovarian carcinoma
Khan, Tashbib, He, Yaowu, Kryza, Thomas, Harrington, Brittney S., Gunter, Jennifer H., Sullivan, Mitchell A., Cuda, Tahleesa, Rogers, Rebecca, Davies, Claire M., Broomfield, Amy, Gough, Madeline, Wu, Andy C., McGann, Thomas, Weroha, S. John, Haluska, Paul, Forbes, Josephine M., Armes, Jane E., Barry, Sinead C., Coward, Jermaine I., Jagasia, Nisha, Chetty, Naven, Snell, Cameron E., Lourie, Rohan, Perrin, Lewis C. and Hooper, John D. (2020). Disruption of glycogen utilization markedly improves the efficacy of carboplatin against preclinical models of clear cell ovarian carcinoma. Cancers, 12 (4) 869, 869. doi: 10.3390/cancers12040869
2020
Journal Article
Anti-CDCP1 immuno-conjugates for detection and inhibition of ovarian cancer
Harrington, Brittney S., He, Yaowu, Khan, Tashbib, Puttick, Simon, Conroy, Paul J., Kryza, Thomas, Cuda, Tahleesa, Sokolowski, Kamil A., Tse, Brian W.C ., Robbins, Katherine K., Arachchige, Buddhika J., Stehbens, Samantha J., Pollock, Pamela M., Reed, Sarah, Weroha, S. John, Haluska, Paul, Salomon, Carlos, Lourie, Rohan, Perrin, Lewis C., Law, Ruby H. P., Whisstock, James C. and Hooper, John D. (2020). Anti-CDCP1 immuno-conjugates for detection and inhibition of ovarian cancer. Theranostics, 10 (5), 2095-2114. doi: 10.7150/thno.30736
2020
Journal Article
Effective targeting of intact and proteolysed CDCP1 for imaging and treatment of pancreatic ductal adenocarcinoma
Kryza, Thomas, Khan, Tashbib, Puttick, Simon, Li, Chao, Sokolowski, Kamil A., Tse, Brian W.C., Cuda, Tahleesa, Lyons, Nicholas, Gough, Madeline, Yin, Julia, Parkin, Ashleigh, Deryugina, Elena I., Quigley, James P., Law, Ruby H.P., Whisstock, James C., Riddell, Andrew D., Barbour, Andrew P., Wyld, David K., Thomas, Paul A., Rose, Stephen, Snell, Cameron E., Pajic, Marina, He, Yaowu and Hooper, John D. (2020). Effective targeting of intact and proteolysed CDCP1 for imaging and treatment of pancreatic ductal adenocarcinoma. Theranostics, 10 (9), 4116-4133. doi: 10.7150/thno.43589
2020
Conference Publication
Thioguanine inhibits colorectal tumorigenesis via β-catenin signalling in intestinal epithelial cells independently of immunosuppression or autophagy
Sheng, Yong H., Giri, Rabina, Davies, Julie, Schreiber, Veronika, Alabbas, Saleh Y., He, Yaowu, Wu, Andy, Hooper, John, Oancea, Iulia, Kijanka, Gregor, Hasnain, Sumaira Z., McGuckin, Michael, Florin, Timothy H. and Begun, Jakob (2020). Thioguanine inhibits colorectal tumorigenesis via β-catenin signalling in intestinal epithelial cells independently of immunosuppression or autophagy. DDW 2020, Online, 2-5 May 2020. Philadelphia, PA United States: Elsevier . doi: 10.1016/s0016-5085(20)30856-8
2019
Conference Publication
Inhibition of Aurora B kinase activity triggers senescence that can be bypassed by blocking p53 and RB function, promoting replication stress
Andrews, Ariel, Kumar, Ramyashree Prasanna, Nazareth, Deborah, Ehmann, Anna, Hooper, John, McMillan, Nigel and Gabrielli, Brian (2019). Inhibition of Aurora B kinase activity triggers senescence that can be bypassed by blocking p53 and RB function, promoting replication stress. AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Therapeutics, Boston, MA USA, 26-30 October 2019. Philadelphia, PA USA: American Association for Cancer Research. doi: 10.1158/1535-7163.TARG-19-A060
2019
Journal Article
The molecular function of kallikrein-related peptidase 14 demonstrates a key modulatory role in advanced prostate cancer
Kryza, Thomas, Bock, Nathalie, Lovell, Scott, Rockstroh, Anja, Lehman, Melanie L., Lesner, Adam, Panchadsaram, Janaththani, Silva, Lakmali Munasinghage, Srinivasan, Srilakshmi, Snell, Cameron E., Williams, Elizabeth D., Fazli, Ladan, Gleave, Martin, Batra, Jyotsna, Nelson, Colleen, Tate, Edward W., Harris, Jonathan, Hooper, John D. and Clements, Judith A. (2019). The molecular function of kallikrein-related peptidase 14 demonstrates a key modulatory role in advanced prostate cancer. Molecular Oncology, 14 (1) 1878-0261.12587, 105-128. doi: 10.1002/1878-0261.12587
2019
Conference Publication
"Theranostics" - radioimmunodetection and radioimmunotherapy for metastatic colorectal cancer in preclinical cancer models
Cuda, T., Thomas, P., Rose, S., Puttick, S. and Hooper, J. (2019). "Theranostics" - radioimmunodetection and radioimmunotherapy for metastatic colorectal cancer in preclinical cancer models. 32nd Annual Congress of the European-Association-of-Nuclear-Medicine (EANM), Barcelona, Spain, 12-16 October 2019. Heidelberg, Germany: Springer.
2019
Journal Article
CDCP1 enhances Wnt signaling in colorectal cancer promoting nuclear localization of β-catenin and E-cadherin
He, Yaowu, Davies, Claire M., Harrington, Brittney S., Hellmers, Linh, Sheng, Yonghua, Broomfield, Amy, McGann, Thomas, Bastick, Kate, Zhong, Laurie, Wu, Andy, Maresh, Grace, McChesney, Shannon, Yau Wong, Kuan, Adams, Mark N., Sullivan, Ryan C., Palmer, James S., Burke, Lez J., Ewing, Adam D., Zhang, Xin, Margolin, David, Li, Li, Lourie, Rohan, Matsika, Admire, Srinivasan, Bhuvana, McGuckin, Michael A., Lumley, John W. and Hooper, John D. (2019). CDCP1 enhances Wnt signaling in colorectal cancer promoting nuclear localization of β-catenin and E-cadherin. Oncogene, 39 (1), 219-233. doi: 10.1038/s41388-019-0983-3
2019
Journal Article
MUC13 promotes the development of colitis-associated colorectal tumors via β-catenin activity
Sheng, Yong Hua, Wong, Kuan Yau, Seim, Inge, Wang, Ran, He, Yaowu, Wu, Andy, Patrick, Maya, Lourie, Rohan, Schreiber, Veronika, Giri, Rabina, Ng, Choa Ping, Popat, Amirali, Hooper, John, Kijanka, Gregor, Florin, Timothy H., Begun, Jakob, Radford, Kristen J., Hasnain, Sumaira and McGuckin, Michael A. (2019). MUC13 promotes the development of colitis-associated colorectal tumors via β-catenin activity. Oncogene, 38 (48), 7294-7310. doi: 10.1038/s41388-019-0951-y
2019
Journal Article
Ovarian cancer-derived exosomes promote tumor metastasis in vivo an effect modulated by the invasiveness capacity of their originating cells
Alharbi, Mona, Lai, Andrew, Guanzon, Dominic, Palma, Carlos, Zuñiga, Felipe, Perrin, Lewis, He, Yaowu, Hooper, John D and Salomon, Carlos (2019). Ovarian cancer-derived exosomes promote tumor metastasis in vivo an effect modulated by the invasiveness capacity of their originating cells. Clinical Science, 133 (13), CS20190082-1419. doi: 10.1042/cs20190082
2019
Conference Publication
GALIUM-68-PSMA-617 AS A NOVEL PET-CT MARKER FOR DISEASE STRATIFYING METASTATIC COLORECTAL CANCER: A PROSPECTIVE PILOT STUDY
Cuda, T., Riddell, A., Thomas, P., Puttick, S. and Hooper, J. (2019). GALIUM-68-PSMA-617 AS A NOVEL PET-CT MARKER FOR DISEASE STRATIFYING METASTATIC COLORECTAL CANCER: A PROSPECTIVE PILOT STUDY. HOBOKEN: WILEY.
2019
Conference Publication
"Theranostics": radioimmunodetection and radioimmunotherapy for metastatic colorectal cancer
Cuda, T., Hooper, J., Puttick, S., He, Y., Kryza, T. and Khan, T. (2019). "Theranostics": radioimmunodetection and radioimmunotherapy for metastatic colorectal cancer. Australian and New Zealand Society of Nuclear Medicine (ANZSNM) 49th ASM, Adelaide, Australia, 26-28 April 2019. Richmond, VIC, Australia: Wiley-Blackwell Publishing Asia.
2019
Conference Publication
Ovarian cancer cell invasiveness regulates their exosomes which induces oncogenic potential and cancer progression in vivo
Alharbi, Mona G., Lai, Andrew, Zuniga, Felipe, Perrin, Lewis, He, Yaowu, Hooper, John D. and Salomon, Carlos (2019). Ovarian cancer cell invasiveness regulates their exosomes which induces oncogenic potential and cancer progression in vivo. 66th Annual Scientific Meeting of the Society for Reproductive Investigation (SRI), Paris, France, March 12-16, 2019. Thousand Oaks, CA, United States: Sage Publications. doi: 10.1177/1933719119834079
2019
Conference Publication
Circulating exosomal miRNAs from women with ovarian cancer for early detection and real-time monitoring of disease progression
Sharma, Shayna, Lai, Andrew, Guanzon, Dominic, Morgan, Terry, Perrin, Lewis C., Hooper, John D. and Salomon, Carlos (2019). Circulating exosomal miRNAs from women with ovarian cancer for early detection and real-time monitoring of disease progression. 66th Annual Scientific Meeting of the Society for Reproductive Investigation (SRI), Paris, France, 12-16 March 2019. Thousand Oaks, CA, United States: Sage Publications. doi: 10.1177/1933719119834079
Funding
Current funding
Supervision
Availability
- Honorary Professor John Hooper is:
- Available for supervision
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Supervision history
Current supervision
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Doctor Philosophy
Factors impacting receptor processing in response to peptide and antibody ligands
Principal Advisor
Other advisors: Dr Jodi Saunus
-
Doctor Philosophy
Molecular and cellular determinants of CDCP1 targeted, payload-delivery antibodies.
Principal Advisor
Other advisors: Associate Professor Michael Landsberg
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Doctor Philosophy
Understanding the function of CDCP1 and its potential as a theranostic target for cholangiocarcinoma
Principal Advisor
Other advisors: Professor Kristofer Thurecht
-
Doctor Philosophy
Investigations into genetic aberrations underlying recurrent ovarian cancer
Principal Advisor
Other advisors: Professor Geoff Faulkner
-
Doctor Philosophy
Novel theranostic targets for metastatic colorectal cancer
Principal Advisor
Other advisors: Professor David Clark
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Doctor Philosophy
Cancer-associated post-translational modifications of the receptor CDCP1 Background:
Principal Advisor
-
Doctor Philosophy
Developing novel strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
-
Doctor Philosophy
Enhancing immune responses to cancer
Associate Advisor
Other advisors: Dr Jazmina Gonzalez Cruz, Professor Brian Gabrielli
-
Doctor Philosophy
Biomarker driven diagnostic and therapeutic innovations in breast cancer
Associate Advisor
-
Doctor Philosophy
Genomic and epigenomic correlates of prostate cancer therapy
Associate Advisor
Other advisors: Associate Professor Adam Ewing
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Doctor Philosophy
Characterisation of EV-associated lipids in the progression of ovarian cancer
Associate Advisor
Other advisors: Dr Dominic Guanzon, Professor Carlos Salomon Gallo, Dr Andrew Lai
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Doctor Philosophy
Developing new strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
Completed supervision
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2022
Doctor Philosophy
Profiling of Immunoglobulin (Ig) G, IgM and IgA Isotype Immune Responses and Development of Autoantibody Biomarkers for Early Detection of Colorectal Cancer
Principal Advisor
-
2022
Doctor Philosophy
Theranostics: Molecular Imaging and Molecularly-Directed Radionuclide Therapy for Metastatic Colorectal Cancer
Principal Advisor
-
2022
Doctor Philosophy
Investigating New Therapeutic Targets for Clear Cell Cancers
Principal Advisor
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2016
Doctor Philosophy
Thesis Title: Investigation into the role of the cell surface glycoprotein CDCP1 in high-grade serous ovarian cancer progression
Principal Advisor
Other advisors: Professor Brian Gabrielli
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2020
Doctor Philosophy
Opportunities for epigenetic therapies in ovarian cancer
Associate Advisor
Other advisors: Associate Professor Jason Lee
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2020
Doctor Philosophy
Regulation of Epithelial Ovarian Cancer Initiation and Progression by Exosomal Proteins and miRNAs
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2020
Doctor Philosophy
New Strategies for Identification of Therapeutic Target of Ovarian Cancer
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2018
Master Philosophy
The effects of Rab13 derived from lymph node stromal cell extracellular vesicles on the pathogenesis of colorectal cancer
Associate Advisor
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2018
Doctor Philosophy
Dysregulation of EGF-trafficking in squamous cell carcinoma and cetuximab resistance
Associate Advisor
Other advisors: Associate Professor Fiona Simpson
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2018
Doctor Philosophy
Targeting Apoptosis as A Novel Therapy for Medulloblastoma
Associate Advisor
Other advisors: Dr Laura Genovesi, Professor Brandon Wainwright
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2010
Doctor Philosophy
Androgen receptor, caveolin-1 and androgen self-sufficiency in prostate cancer
Associate Advisor
Other advisors: Professor David Johnson
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