Overview
Availability
- Professor Esteban Marcellin is:
- Available for supervision
Fields of research
Qualifications
- Bachelor (Honours) of Chemical Engineering, Universidad Iberoamericana
- Doctor of Philosophy, The University of Queensland
Research interests
-
Systems and Synthetic Biology
Systems and synthetic biology are powerful tools to guide the improvement of biological systems for producing fuels, chemicals, foods and biopharmaceuticals. Systems and synthetic approaches can be used to identify metabolic engineering targets and develop new production strains. Using data-driven approaches that take advantage of the tremendous growth in high throughput ‘-omics (genomics, transcriptomics, proteomics and metabolomics) coupled with mathematical models we can better understand cells. The data gathered can be used to suggest potential metabolic engineering strategies (e.g. bottlenecks that can be targeted for knockout/up-regulation) to improve yields and productivity. Biological systems are refined and improved using biological data, leading to further iterative rounds of metabolic engineering to enhance production. Using gas fermentation, we are consuming greenhouse gases (CO and CO2) as feedstock for fermentation and converting those greenhouse gases into valuable fuels, chemicals and fuels. This allows us to decrease our dependence on finite fossil fuel resources and improves sustainability for Australia. Our projects aim at producing efficient cell lines to increase the yields and productivity of biological systems. In collaboration with leaders in the industry, such as Lanzatech, Amyris, Zoetis, Dow, CSL and Thermo-Fisher, we are applying systems metabolic engineering to enhance yields and increase the production scope of the advanced biomanufacturing sector.
Works
Search Professor Esteban Marcellin’s works on UQ eSpace
2022
Journal Article
Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: implications for baculovirus expression
Malmanche, Henry, Marcellin, Esteban and Reid, Steven (2022). Knockout of Sf‐Caspase‐1 generates apoptosis‐resistant Sf9 cell lines: implications for baculovirus expression. Biotechnology Journal, 17 (7) 2100532, 2100532. doi: 10.1002/biot.202100532
2022
Conference Publication
Engineering novel regulation factors in Clostridium autoethanogenum using rationale from a test-system in Escherichia coli
Heffernan, James, Gonzalez Garcia, Axa, Warden, Andrew, Nielsen, Lars K. and Marcellin, Esteban (2022). Engineering novel regulation factors in Clostridium autoethanogenum using rationale from a test-system in Escherichia coli. Centre of Excellence in Synthetic Biology Annual Conference, Australia, 3-6 April 2022.
2022
Journal Article
A genome-scale metabolic model of Methanoperedens nitroreducens: assessing bioenergetics and thermodynamic feasibility
He, Bingqing, Cai, Chen, McCubbin, Tim, Muriel, Jorge Carrasco, Sonnenschein, Nikolaus, Hu, Shihu, Yuan, Zhiguo and Marcellin, Esteban (2022). A genome-scale metabolic model of Methanoperedens nitroreducens: assessing bioenergetics and thermodynamic feasibility. Metabolites, 12 (4) 314, 314. doi: 10.3390/metabo12040314
2022
Journal Article
Absolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum
Valgepea, Kaspar, Talbo, Gert, Takemori, Nobuaki, Takemori, Ayako, Ludwig, Christina, Mahamkali, Vishnuvardhan, Mueller, Alexander P., Tappel, Ryan, Köpke, Michael, Simpson, Séan Dennis, Nielsen, Lars Keld and Marcellin, Esteban (2022). Absolute proteome quantification in the gas-fermenting acetogen Clostridium autoethanogenum. mSystems, 7 (2), 1. doi: 10.1128/msystems.00026-22
2022
Journal Article
Modelling apoptosis resistance in CHO cells with CRISPR‐mediated knock‐outs of Bak1, Bax, and Bok
MacDonald, Michael A., Barry, Craig, Groves, Teddy, Martínez, Verónica S., Gray, Peter P., Baker, Kym, Shave, Evan, Mahler, Stephen, Munro, Trent, Marcellin, Esteban and Nielsen, Lars K. (2022). Modelling apoptosis resistance in CHO cells with CRISPR‐mediated knock‐outs of Bak1, Bax, and Bok. Biotechnology and Bioengineering, 119 (6), 1380-1391. doi: 10.1002/bit.28062
2022
Journal Article
Multi-omic characterisation of Streptomyces hygroscopicus NRRL 30439: detailed assessment of its secondary metabolic potential
Barry, Craig P., Gillane, Rosemary, Talbo, Gert H., Plan, Manual, Palfreyman, Robin, Haber-Stuk, Andrea K., Power, John, Nielsen, Lars K. and Marcellin, Esteban (2022). Multi-omic characterisation of Streptomyces hygroscopicus NRRL 30439: detailed assessment of its secondary metabolic potential. Molecular Omics, 18 (3), 226-236. doi: 10.1039/d1mo00150g
2022
Journal Article
Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy
Pavan, Marilene, Reinmets, Kristina, Garg, Shivani, Mueller, Alexander P., Marcellin, Esteban, Köpke, Michael and Valgepea, Kaspar (2022). Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy. Metabolic Engineering, 71, 117-141. doi: 10.1016/j.ymben.2022.01.015
2021
Journal Article
High methanol‐to‐formate ratios induce butanol production in Eubacterium limosum
Wood, Jamin C., Marcellin, Esteban, Plan, Manuel R. and Virdis, Bernardino (2021). High methanol‐to‐formate ratios induce butanol production in Eubacterium limosum. Microbial Biotechnology, 15 (5), 1542-1549. doi: 10.1111/1751-7915.13963
2021
Journal Article
Strategies to improve viability of a circular carbon bioeconomy – A techno-economic review of microbial electrosynthesis and gas fermentation
Wood, Jamin C., Grové, Johannes, Marcellin, Esteban, Heffernan, James K., Hu, Shihu, Yuan, Zhiguo and Virdis, Bernardino (2021). Strategies to improve viability of a circular carbon bioeconomy – A techno-economic review of microbial electrosynthesis and gas fermentation. Water Research, 201 117306, 1-14. doi: 10.1016/j.watres.2021.117306
2021
Journal Article
Roles and opportunities for microbial anaerobic oxidation of methane in natural and engineered systems
Cai, Chen, Zhang, Xueqin, Wu, Mengxiong, Liu, Tao, Lai, Chun-Yu, Frank, Joshua, He, Bingqing, Marcellin, Esteban, Guo, Jianhua, Hu, Shihu and Yuan, Zhiguo (2021). Roles and opportunities for microbial anaerobic oxidation of methane in natural and engineered systems. Energy and Environmental Science, 14 (9), 4803-4830. doi: 10.1039/d1ee00708d
2021
Journal Article
Comparative Economic Analysis Between Endogenous and Recombinant Production of Hyaluronic Acid
Torres-Acosta, Mario A., Castaneda-Aponte, Héctor M., Mora-Galvez, Liliana M., Gil-Garzon, Monica R., Banda-Magaña, Martin P., Marcellin, Esteban, Mayolo-Deloisa, Karla and Licona-Cassani, Cuauhtemoc (2021). Comparative Economic Analysis Between Endogenous and Recombinant Production of Hyaluronic Acid. Frontiers in Bioengineering and Biotechnology, 9 680278, 680278. doi: 10.3389/fbioe.2021.680278
2021
Journal Article
Network analyses predict small RNAs that might modulate gene expression in the testis and epididymis of Bos indicus bulls
de Lima, Andressa O., Afonso, Juliana, Edson, Janette, Marcellin, Esteban, Palfreyman, Robin, Porto-Neto, Laercio R., Reverter, Antonio and Fortes, Marina R. S. (2021). Network analyses predict small RNAs that might modulate gene expression in the testis and epididymis of Bos indicus bulls. Frontiers in Genetics, 12 610116, 610116. doi: 10.3389/fgene.2021.610116
2021
Journal Article
Towards sustainable bioinoculants: a fermentation strategy for high cell density cultivation of Paraburkholderia sp. SOS3, a plant growth-promoting bacterium isolated in Queensland, Australia
Petersen, Ian, Paungfoo-Lonhienne, Chanyarat, Marcellin, Esteban, Nielsen, Lars Keld and Gonzalez, Axayacatl (2021). Towards sustainable bioinoculants: a fermentation strategy for high cell density cultivation of Paraburkholderia sp. SOS3, a plant growth-promoting bacterium isolated in Queensland, Australia. Fermentation, 7 (2) 58, 1-17. doi: 10.3390/fermentation7020058
2021
Journal Article
Cyclic di-AMP oversight of counter-Ion osmolyte pools impacts intrinsic cefuroxime resistance in Lactococcus lactis
Pham, Huong Thi, Shi, Wen, Xiang, Yuwei, Foo, Su Yi, Plan, Manuel R., Courtin, Pascal, Chapot-Chartier, Marie-Pierre, Smid, Eddy J., Liang, Zhao-Xun, Marcellin, Esteban and Turner, Mark S. (2021). Cyclic di-AMP oversight of counter-Ion osmolyte pools impacts intrinsic cefuroxime resistance in Lactococcus lactis. mBio, 12 (2) e00324-21, 1-18. doi: 10.1128/mBio.00324-21
2021
Journal Article
multiTFA: a Python package for multi-variate thermodynamics-based flux analysis
Mahamkali, Vishnuvardhan, McCubbin, Tim, Beber, Moritz Emanuel, Noor, Elad, Marcellin, Esteban and Nielsen, Lars Keld (2021). multiTFA: a Python package for multi-variate thermodynamics-based flux analysis. Bioinformatics, 37 (18), 3064-3066. doi: 10.1093/bioinformatics/btab151
2021
Journal Article
Transcriptional control of Clostridium autoethanogenum using CRISPRi
Fackler, Nick, Heffernan, James, Juminaga, Alex, Doser, Damien, Nagaraju, Shilpa, Gonzalez-Garcia, R. Axayacatl, Simpson, Séan D., Marcellin, Esteban and Köpke, Michael (2021). Transcriptional control of Clostridium autoethanogenum using CRISPRi. Synthetic Biology, 6 (1) ysab008, 1-8. doi: 10.1093/synbio/ysab008
2021
Journal Article
Perfusion culture of Chinese Hamster Ovary cells for bioprocessing applications
MacDonald, Michael A., Nöbel, Matthias, Roche Recinos, Dinora, Martínez, Verónica S., Schulz, Benjamin L., Howard, Christopher B., Baker, Kym, Shave, Evan, Lee, Yih Yean, Marcellin, Esteban, Mahler, Stephen, Nielsen, Lars Keld and Munro, Trent (2021). Perfusion culture of Chinese Hamster Ovary cells for bioprocessing applications. Critical Reviews in Biotechnology, 42 (7), 1-17. doi: 10.1080/07388551.2021.1998821
2020
Journal Article
Time-course transcriptomics reveals that amino acids catabolism plays a key role in toxinogenesis and morphology in Clostridium tetani
Orellana, Camila A., Zaragoza, Nicolas E., Licona-Cassani, Cuauhtemoc, Palfreyman, Robin W., Cowie, Nicholas, Moonen, Glenn, Moutafis, George, Power, John, Nielsen, Lars K. and Marcellin, Esteban (2020). Time-course transcriptomics reveals that amino acids catabolism plays a key role in toxinogenesis and morphology in Clostridium tetani. Journal of Industrial Microbiology and Biotechnology, 47 (12), 1059-1073. doi: 10.1007/s10295-020-02330-3
2020
Journal Article
Enhanced metal recovery by efficient agglomeration of precipitates in an up-flow fixed-bed bioreactor
Liu, Yun, Vaughan, James, Southam, Gordon, Serrano, Antonio, Marcellin, Esteban, Nancucheo, Ivan and Villa-Gomez, Denys K. (2020). Enhanced metal recovery by efficient agglomeration of precipitates in an up-flow fixed-bed bioreactor. Chemical Engineering Journal, 416 127662, 127662. doi: 10.1016/j.cej.2020.127662
2020
Journal Article
Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae
Espinosa, Monica I., Gonzalez-Garcia, Ricardo A., Valgepea, Kaspar, Plan, Manuel R., Scott, Colin, Pretorius, Isak S., Marcellin, Esteban, Paulsen, Ian T. and Williams, Thomas C. (2020). Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae. Nature Communications, 11 (1) 5564, 5564. doi: 10.1038/s41467-020-19390-9
Funding
Current funding
Supervision
Availability
- Professor Esteban Marcellin is:
- Available for supervision
Before you email them, read our advice on how to contact a supervisor.
Supervision history
Current supervision
-
Doctor Philosophy
Optimizing Gas Fermentation for the Valorization of Mixed Gases into High-Value Bio-Products
Principal Advisor
Other advisors: Dr James Heffernan
-
Doctor Philosophy
Accelerating the design of cell lines for biologic production
Principal Advisor
Other advisors: Dr Marianne Gillard, Professor Timothy Mercer
-
Doctor Philosophy
Biorecycyling carbon using acetogens
Principal Advisor
Other advisors: Professor Lars Nielsen, Professor Gary Schenk, Dr Axa Gonzalez, Dr James Heffernan
-
Doctor Philosophy
Modelling cyanobacterial metabolism for the production of high-value products
Principal Advisor
-
Doctor Philosophy
Modelling cyanobacterial metabolism for the production of high-value products
Principal Advisor
-
Doctor Philosophy
Profitable Upcycling of GM Biomass from Precision Fermentation
Principal Advisor
Other advisors: Dr Denys Villa Gomez
-
Master Philosophy
Harnessing Microbial Power: Turning Carbon into Valuable Products through Gas Fermentation
Principal Advisor
Other advisors: Dr James Heffernan
-
Doctor Philosophy
Biological CO2 and H2 utilization for sustainable animal feed production
Principal Advisor
Other advisors: Professor Lars Nielsen, Dr James Heffernan
-
Doctor Philosophy
Pathway engineering and systems biology for optimised isobutanol production in gas-fermenting acetogens: From strain isolation to metabolic limitations identification
Principal Advisor
Other advisors: Professor Lars Nielsen, Professor Gary Schenk, Dr Axa Gonzalez, Dr James Heffernan
-
Doctor Philosophy
Production of the Mannopeptimycin Antibiotic and other unknown Biosynthetic Gene Clusters from Streptomyces hygroscopicus
Principal Advisor
Other advisors: Dr Zeinab Khalil
-
Doctor Philosophy
Engineering cyanobacteria for high-value flavours and fragrances production
Principal Advisor
-
Doctor Philosophy
Optimizing Mixed Gas Fermentation for Enhanced Biofuel Production
Principal Advisor
Other advisors: Dr James Heffernan, Dr Yosephine Gumulya
-
Doctor Philosophy
Harnessing Microbal Power: Turning Carbon into valuable Produscts through gas fermentation
Principal Advisor
Other advisors: Dr James Heffernan
-
Doctor Philosophy
Biorecycyling carbon using acetogens
Principal Advisor
Other advisors: Professor Lars Nielsen, Professor Gary Schenk, Dr Axa Gonzalez, Dr James Heffernan
-
Doctor Philosophy
Engineering Eubacterium limosum for Enhanced CO2 Fixation via rational genome-scale
Principal Advisor
Other advisors: Dr James Heffernan
-
Doctor Philosophy
Greenhouse Gas Fermentation and Computational Fluid Dynamics (CFD)
Principal Advisor
Other advisors: Dr James Heffernan
-
Doctor Philosophy
Harnessing Microbal Power: Turning Carbon into valuable Produscts through gas fermentation
Principal Advisor
Other advisors: Dr James Heffernan
-
Doctor Philosophy
Engineering cyanobacteria for high-value flavours and fragrances production
Principal Advisor
-
Doctor Philosophy
Co-assimilation of C1 compounds (methanol, formate, formaldehyde) and components from lignocellulosic feedstock
Associate Advisor
Other advisors: Dr Birgitta Ebert
-
Doctor Philosophy
Systems biology-guided cell line engineering for production of therapeutic proteins with complex post-translational modifications
Associate Advisor
Other advisors: Professor Benjamin Schulz
-
Doctor Philosophy
EnzOnomy - an enzyme-based production pipeline for the bioeconomy
Associate Advisor
Other advisors: Professor Damian Hine, Professor Gary Schenk
-
Doctor Philosophy
Advancing biofertiliser for sustainable agriculture
Associate Advisor
Other advisors: Professor Susanne Schmidt, Dr Axa Gonzalez
-
Doctor Philosophy
Leveraging Biomass Waste: Exploiting Proteins from Precision Fermentation Bioprocesses
Associate Advisor
Other advisors: Dr Huadong Peng
-
Doctor Philosophy
Accelerating the extraction of critical metals such as rare earth elements and gallium from red mud tailings.
Associate Advisor
Other advisors: Dr Denys Villa Gomez
-
Doctor Philosophy
Microbial fatty acid derivative production from CO2-derived C2 intermediates
Associate Advisor
Other advisors: Dr Birgitta Ebert
-
Doctor Philosophy
Mechanisms of osmoresistance in industrial Lactococcus cemoris
Associate Advisor
Other advisors: Professor Mark Turner
-
Doctor Philosophy
Systems biology-guided cell line engineering for production of therapeutic proteins with complex post-translational modifications
Associate Advisor
Other advisors: Professor Benjamin Schulz
-
Doctor Philosophy
Influence of structurally different polysaccharides and their interactions with saliva and tannins on mouthfeel in wine
Associate Advisor
Other advisors: Dr Marlize Bekker, Dr Huadong Peng, Professor Heather Smyth
-
Doctor Philosophy
Engineering biology of yeast for sustainable fragrance production through precision fermentation
Associate Advisor
Other advisors: Dr Axa Gonzalez, Dr Huadong Peng
-
Doctor Philosophy
Advancing biofertiliser for sustainable agriculture
Associate Advisor
Other advisors: Professor Susanne Schmidt, Dr Axa Gonzalez
-
Doctor Philosophy
Using synthetic biology to accelerate critical metals recovery from mine waste
Associate Advisor
Other advisors: Dr Denys Villa Gomez
Completed supervision
-
2024
Doctor Philosophy
Production of the Mannopeptimycin Antibiotic and Other Unknown Biosynthetic Gene Clusters from Streptomyces hygroscopicus
Principal Advisor
Other advisors: Dr Zeinab Khalil
-
2023
Doctor Philosophy
Systems metabolic engineering for one-carbon (C1) utilization metabolism
Principal Advisor
Other advisors: Honorary Professor Zhiguo Yuan
-
2023
Doctor Philosophy
Sustainable fuel and chemical production via Gas Fermentation: exploring CO2 metabolism by Clostridium autoethanogenum
Principal Advisor
Other advisors: Professor Lars Nielsen, Dr Axa Gonzalez
-
2023
Doctor Philosophy
Modelling and characterising life and death of mammalian suspension cultures
Principal Advisor
Other advisors: Professor Lars Nielsen, Dr Veronica Martinez Salazar, Dr Gert Talbo
-
2022
Doctor Philosophy
Cell Line Development for High Density Culture of CHO Cells in Perfusion: Applications of Apoptosis Resistance
Principal Advisor
Other advisors: Professor Peter Gray, Dr Veronica Martinez Salazar
-
2021
Doctor Philosophy
Insight into acetogens metabolism through the use of thermodynamic metabolic flux analysis
Principal Advisor
Other advisors: Professor Lars Nielsen
-
2020
Doctor Philosophy
Understanding toxin production in Clostridium tetani: a systems approach
Principal Advisor
Other advisors: Professor Lars Nielsen
-
2019
Doctor Philosophy
From waste to chemicals: On the metabolism of Clostridium autoethanogenum and PHB production from gas fermentation
Principal Advisor
Other advisors: Professor Lars Nielsen
-
2018
Doctor Philosophy
Exploring the metabolism of propionibacteria using genome-scale modelling
Principal Advisor
Other advisors: Professor Lars Nielsen
-
2018
Doctor Philosophy
Metabolic Engineering of propionibacteria's Wood Werkman cycle in Escherichia coli and Saccharomyces cerevisiae
Principal Advisor
Other advisors: Professor Lars Nielsen, Professor Mark Turner
-
2017
Doctor Philosophy
Understanding high propionic acid production in Propionibacterium
Principal Advisor
Other advisors: Professor Lars Nielsen
-
2025
Doctor Philosophy
Systems biology-guided cell line engineering for production of therapeutic proteins with complex post-translational modifications
Associate Advisor
Other advisors: Dr Lucia Zacchi, Professor Benjamin Schulz
-
2024
Doctor Philosophy
Mechanisms of osmoresistance in industrial Lactococcus cemoris
Associate Advisor
Other advisors: Dr Huong Thi Pham, Professor Mark Turner
-
2023
Doctor Philosophy
Exploring Metabolic Demands of High Cell Density Chinese Hamster Ovary Cell Culture - Studying Apoptosis Resistance in Perfusion Culture
Associate Advisor
Other advisors: Dr Gert Talbo, Dr Veronica Martinez Salazar
-
2023
Doctor Philosophy
Production of liquid chemicals from C1 feedstocks using Eubacterium limosum
Associate Advisor
Other advisors: Dr Bernardino Virdis
-
2022
Doctor Philosophy
Sf9 cell line engineering to improve baculovirus expression
Associate Advisor
-
2016
Doctor Philosophy
Understanding CHO cells biology for enhanced biopharmaceutical production: a comparative transcriptomic and proteomic approach
Associate Advisor
Other advisors: Professor Peter Gray, Professor Lars Nielsen
-
2015
Doctor Philosophy
Quantitative bacterial phosphoproteomics
Associate Advisor
Other advisors: Professor Lars Nielsen
-
2014
Doctor Philosophy
Characterisation of the Saccharopolyspora erythraea metabolic switch: a systems approach
Associate Advisor
Other advisors: Professor Lars Nielsen
Media
Enquiries
For media enquiries about Professor Esteban Marcellin's areas of expertise, story ideas and help finding experts, contact our Media team: