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Dr Anne Lagendijk
Dr

Anne Lagendijk

Email: 
Phone: 
+61 7 334 62105

Overview

Availability

Dr Anne Lagendijk is:
Available for supervision

Qualifications

  • Doctor of Philosophy, Utrecht University

Research interests

  • Cellular mechanisms to maintain a healthy vasculature

    Our vascular system transports approximately 7500 liters of blood each day. Arteries deliver oxygen and nutrients throughout the body after which the venous system returns the deoxygenated blood back to the heart. Architecturally, these blood vessels are extremely heterogeneous. The Lagendijk group investigates the development and maintenance of a functional blood vessel network in zebrafish and bioengineered human microvessels. The cells that make up our blood vessels continuously adapt their size, adhesiveness and compliance order to ensure the right balance between vessel integrity and permeability in a context dependent manner. Mechanical cues play a major role in the functional adaptation of blood vessels. Despite ongoing research unraveling the structural components of mechanical hubs in the cells, it is essential to assess the magnitude of forces that are transduced at these sites and the biological consequences for vessel function. Dr. Lagendijk has previously developed a VE-cadherin tension biosensor line in zebrafish. This line provides the first vertebrate model that reports intra-molecular tension and was utilised to identify changes in junctional organisation and VE-cadherin tension that occur as arteries mature and revealed molecular pathways that allow for this maturation to happen. In addition, the lab has established disease models for vascular malformations that are known to lead to neurological deficits and stroke. Modelling in zebrafish allows analyses of the initiating mechanisms of these vascular pathologies at unprecedented cellular and subcellular resolution.

Works

Search Professor Anne Lagendijk’s works on UQ eSpace

49 works between 2006 and 2024

1 - 20 of 49 works

2024

Journal Article

c-Src induced vascular malformations require localised matrix degradation at focal adhesions

Essebier, Patricia, Keyser, Mikaela, Yordanov, Teodor, Hill, Brittany, Yu, Alexander, Noordstra, Ivar, Yap, Alpha S., Stehbens, Samantha J., Lagendijk, Anne K., Schimmel, Lilian and Gordon, Emma J. (2024). c-Src induced vascular malformations require localised matrix degradation at focal adhesions. Journal of Cell Science, 137 (13) jcs262101. doi: 10.1242/jcs.262101

c-Src induced vascular malformations require localised matrix degradation at focal adhesions

2024

Journal Article

Hyaluronic acid turnover controls the severity of cerebral cavernous malformations in bioengineered human micro-vessels

Yordanov, Teodor E., Keyser, Mikaela S., Enriquez Martinez, Marco A., Esposito, Tyron, Tefft, Juliann B., Morris, Elysse K., Labzin, Larisa I., Stehbens, Samantha J., Rowan, Alan E., Hogan, Benjamin M., Chen, Christopher S., Lauko, Jan and Lagendijk, Anne K. (2024). Hyaluronic acid turnover controls the severity of cerebral cavernous malformations in bioengineered human micro-vessels. APL Bioengineering, 8 (1) 016108, 016108. doi: 10.1063/5.0159330

Hyaluronic acid turnover controls the severity of cerebral cavernous malformations in bioengineered human micro-vessels

2023

Conference Publication

Uncovering Dynamic Changes In Medulloblastoma Associated Vasculature In Zebrafish

Morris, Elysse, Daignault-Mill, Sheena, Ju, Robert J., da Silva, Jason, Gordon, Emma, Wainwright, Brandon, Stehbens, Samantha, Genovesi, Laura and Lagendijk, Anne (2023). Uncovering Dynamic Changes In Medulloblastoma Associated Vasculature In Zebrafish. 2023 Pediatric Neuro-Oncology Research Conference, Washington, DC United States, 22-24 June 2023. Oxford, United Kingdom: Oxford University Press. doi: 10.1093/neuonc/noad073.274

Uncovering Dynamic Changes In Medulloblastoma Associated Vasculature In Zebrafish

2023

Journal Article

Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

Grimm, Lin, Mason, Elizabeth, Yu, Hujun, Dudczig, Stefanie, Panara, Virginia, Chen, Tyrone, Bower, Neil I., Paterson, Scott, Rondon Galeano, Maria, Kobayashi, Sakurako, Senabouth, Anne, Lagendijk, Anne K., Powell, Joseph, Smith, Kelly A., Okuda, Kazuhide S., Koltowska, Katarzyna and Hogan, Benjamin M. (2023). Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development. The EMBO Journal, 42 (11) e112590, e112590. doi: 10.15252/embj.2022112590

Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development

2023

Journal Article

Macrophage ACE2 is necessary for SARS-CoV-2 replication and subsequent cytokine responses that restrict continued virion release

Labzin, Larisa I., Chew, Keng Yih, Eschke, Kathrin, Wang, Xiaohui, Esposito, Tyron, Stocks, Claudia J., Rae, James, Patrick, Ralph, Mostafavi, Helen, Hill, Brittany, Yordanov, Teodor E., Holley, Caroline L., Emming, Stefan, Fritzlar, Svenja, Mordant, Francesca L., Steinfort, Daniel P., Subbarao, Kanta, Nefzger, Christian M., Lagendijk, Anne K., Gordon, Emma J., Parton, Robert G., Short, Kirsty R., Londrigan, Sarah L. and Schroder, Kate (2023). Macrophage ACE2 is necessary for SARS-CoV-2 replication and subsequent cytokine responses that restrict continued virion release. Science Signaling, 16 (782) eabq1366, eabq1366. doi: 10.1126/scisignal.abq1366

Macrophage ACE2 is necessary for SARS-CoV-2 replication and subsequent cytokine responses that restrict continued virion release

2023

Journal Article

Addressing blood-brain-tumor-barrier heterogeneity in pediatric brain tumors with innovative preclinical models

Morris, Elysse K., Daignault-Mill, Sheena, Stehbens, Samantha J., Genovesi, Laura A. and Lagendijk, Anne K. (2023). Addressing blood-brain-tumor-barrier heterogeneity in pediatric brain tumors with innovative preclinical models. Frontiers in Oncology, 13 1101522, 1-9. doi: 10.3389/fonc.2023.1101522

Addressing blood-brain-tumor-barrier heterogeneity in pediatric brain tumors with innovative preclinical models

2022

Journal Article

Dynamically regulated focal adhesions coordinate endothelial cell remodelling in developing vasculature

Chau, Tevin C. Y., Keyser, Mikaela S., Da Silva, Jason A., Morris, Elysse K., Yordanov, Teodor E., Duscyz, Kinga P., Paterson, Scott, Yap, Alpha S., Hogan, Benjamin M. and Lagendijk, Anne Karine (2022). Dynamically regulated focal adhesions coordinate endothelial cell remodelling in developing vasculature. Development, 149 (23) dev200454, 1-14. doi: 10.1242/dev.200454

Dynamically regulated focal adhesions coordinate endothelial cell remodelling in developing vasculature

2022

Journal Article

The people behind the papers – Tevin Chau and Anne Lagendijk

Lagendijk, Anne and Chau, Tevin (2022). The people behind the papers – Tevin Chau and Anne Lagendijk. Boletin de Malariologia y Salud Ambiental, 149 (23) dev201441. doi: 10.1242/dev.201441

The people behind the papers – Tevin Chau and Anne Lagendijk

2022

Journal Article

Editorial: Forces in biology - Cell and developmental mechanobiology and its implications in disease - Volume II

Wu, Selwin K., Gomez, Guillermo A., Stehbens, Samantha, Acharya, Bipul R., Ratheesh, Aparna, Priya, Rashmi, Lagendijk, Anne and Bershadsky, Alexander (2022). Editorial: Forces in biology - Cell and developmental mechanobiology and its implications in disease - Volume II. Frontiers in Cell and Developmental Biology, 10 1082857, 1082857. doi: 10.3389/fcell.2022.1082857

Editorial: Forces in biology - Cell and developmental mechanobiology and its implications in disease - Volume II

2022

Journal Article

Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection

Gibson, Josie F., Bojarczuk, Aleksandra, Evans, Robert J., Kamuyango, Alfred Alinafe, Hotham, Richard, Lagendijk, Anne K., Hogan, Benjamin M., Ingham, Philip W., Renshaw, Stephen A. and Johnston, Simon A. (2022). Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection. PLoS pathogens, 18 (4) e1010389, e1010389. doi: 10.1371/journal.ppat.1010389

Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection

2021

Journal Article

The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

Koltowska, Katarzyna, Okuda, Kazuhide S., Gloger, Marleen, Rondon-Galeano, Maria, Mason, Elizabeth, Xuan, Jiachen, Dudczig, Stefanie, Chen, Huijun, Arnold, Hannah, Skoczylas, Renae, Bower, Neil I., Paterson, Scott, Lagendijk, Anne Karine, Baillie, Gregory J., Leshchiner, Ignaty, Simons, Cas, Smith, Kelly A., Goessling, Wolfram, Heath, Joan K., Pearson, Richard B., Sanij, Elaine, Schulte-Merker, Stefan and Hogan, Benjamin M. (2021). The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function. Nature Cell Biology, 23 (11), 1136-1147. doi: 10.1038/s41556-021-00784-w

The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function

2021

Journal Article

Endothelial cells are not productively infected by SARS‐CoV‐2

Schimmel, Lilian, Chew, Keng Yih, Stocks, Claudia J., Yordanov, Teodor E., Essebier, Patricia, Kulasinghe, Arutha, Monkman, James, Santos Miggiolaro, Anna Flavia Ribeiro, Cooper, Caroline, Noronha, Lucia, Schroder, Kate, Lagendijk, Anne Karine, Labzin, Larisa I., Short, Kirsty R. and Gordon, Emma J. (2021). Endothelial cells are not productively infected by SARS‐CoV‐2. Clinical and Translational Immunology, 10 (10) e1350, e1350. doi: 10.1002/cti2.1350

Endothelial cells are not productively infected by SARS‐CoV‐2

2021

Journal Article

Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice

Chau, Tevin CY., Baek, Sungmin, Coxam, Baptiste, Skoczylas, Renae, Rondon‐Galeano, Maria, Bower, Neil I., Wainwright, Elanor N., Stacker, Steven A., Cooper, Helen M., Koopman, Peter A., Lagendijk, Anne K., Harvey, Natasha L., François, Mathias and Hogan, Benjamin M. (2021). Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice. Developmental Dynamics, 251 (2) dvdy.390, 336-349. doi: 10.1002/dvdy.390

Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice

2021

Journal Article

Mechanotransduction activates RhoA in the neighbors of apoptotic epithelial cells to engage apical extrusion

Duszyc, Kinga, Gomez, Guillermo A., Lagendijk, Anne K., Yau, Mei-Kwan, Nanavati, Bageshri Naimish, Gliddon, Briony L., Hall, Thomas E., Verma, Suzie, Hogan, Benjamin M., Pitson, Stuart M., Fairlie, David P., Parton, Robert G. and Yap, Alpha S. (2021). Mechanotransduction activates RhoA in the neighbors of apoptotic epithelial cells to engage apical extrusion. Current Biology, 31 (6), 1326-1336.e5. doi: 10.1016/j.cub.2021.01.003

Mechanotransduction activates RhoA in the neighbors of apoptotic epithelial cells to engage apical extrusion

2020

Journal Article

Patient derived orthotopic xenograft models of Medulloblastoma lack a functional blood brain barrier

Genovesi, Laura A., Puttick, Simon, Millar, Amanda, Kojic, Marija, Ji, Pengxiang, Lagendijk, Anne K., Brighi, Caterina, Bonder, Claudine S, Adolphe, Christelle and Wainwright, Brandon J. (2020). Patient derived orthotopic xenograft models of Medulloblastoma lack a functional blood brain barrier. Neuro-Oncology, 23 (5), 732-742. doi: 10.1093/neuonc/noaa266

Patient derived orthotopic xenograft models of Medulloblastoma lack a functional blood brain barrier

2020

Journal Article

Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion

Teo, Jessica L, Tomatis, Vanesa M, Coburn, Luke, Lagendijk, Anne K, Schouwenaar, Irin-Maya, Budnar, Srikanth, Hall, Thomas E, Verma, Suzie, McLachlan, Robert W, Hogan, Benjamin M, Parton, Robert G, Yap, Alpha S and Gomez, Guillermo A (2020). Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion. Molecular Biology of the Cell, 31 (23), 2495-2629. doi: 10.1091/mbc.E20-01-0084

Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion

2020

Journal Article

Localised Collagen2a1 secretion supports lymphatic endothelial cell migration in the zebrafish embryo

Chaudhury, Smrita, Okuda, Kazuhide S., Koltowska, Katarzyna, Lagendijk, Anne K., Paterson, Scott, Baillie, Gregory J., Simons, Cas, Smith, Kelly A., Hogan, Benjamin M. and Bower, Neil I. (2020). Localised Collagen2a1 secretion supports lymphatic endothelial cell migration in the zebrafish embryo. Development, 147 (18) dev190983, dev.190983. doi: 10.1242/dev.190983

Localised Collagen2a1 secretion supports lymphatic endothelial cell migration in the zebrafish embryo

2019

Journal Article

The alternative splicing regulator Nova2 constrains vascular Erk signaling to limit specification of the lymphatic lineage

Baek, Sungmin, Oh, Tae Gyu, Secker, Genevieve, Sutton, Drew L., Okuda, Kazuhide S., Paterson, Scott, Bower, Neil I., Toubia, John, Koltowska, Katarzyna, Capon, Samuel J., Baillie, Gregory J., Simons, Cas, Muscat, George E.O., Lagendijk, Anne K., Smith, Kelly A., Harvey, Natasha L. and Hogan, Benjamin M. (2019). The alternative splicing regulator Nova2 constrains vascular Erk signaling to limit specification of the lymphatic lineage. Developmental Cell, 49 (2), 279-292. doi: 10.1016/j.devcel.2019.03.017

The alternative splicing regulator Nova2 constrains vascular Erk signaling to limit specification of the lymphatic lineage

2019

Journal Article

Peri-arterial specification of vascular mural cells from naive mesenchyme requires Notch signaling

Ando, Koji, Wang, Weili, Peng, Di, Chiba, Ayano, Lagendijk, Anne K., Barske, Lindsey, Crump, J. Gage, Stainier, Didier Y. R., Lendahl, Urban, Koltowska, Katarzyna, Hogan, Benjamin M., Fukuhara, Shigetomo, Mochizuki, Naoki and Betsholtz, Christer (2019). Peri-arterial specification of vascular mural cells from naive mesenchyme requires Notch signaling. Development, 146 (2) dev165589, dev165589. doi: 10.1242/dev.165589

Peri-arterial specification of vascular mural cells from naive mesenchyme requires Notch signaling

2018

Journal Article

Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

Paatero, Ilkka, Sauteur, Loïc, Lee, Minkyoung, Lagendijk, Anne K., Heutschi, Daniel, Wiesner, Cora, Guzmán, Camilo, Bieli, Dimitri, Hogan, Benjamin M., Affolter, Markus and Belting, Heinz-Georg (2018). Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature Communications, 9 (1) 3545, 3545. doi: 10.1038/s41467-018-05851-9

Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction

Funding

Current funding

  • 2024 - 2027
    Discovering therapeutic vulnerabilities of circulating melanoma clusters
    United States Congressionally Directed Medical Research Programs - Melanoma Research Program
    Open grant
  • 2024 - 2027
    The impact of Hyaluronic Acid on growth factor signalling and angiogenesis (ARC Discovery Project administered by the University of Melbourne)
    University of Melbourne
    Open grant
  • 2024 - 2027
    Validating new druggable pathways for cerebral cavernous malformations
    NHMRC IDEAS Grants
    Open grant
  • 2023 - 2026
    Identifying genetic and lifestyle determinants of abdominal aortic calcification that predispose to cardiovascular disease (MRFF Cardiovascular Health Mission Grant administered by Edith Cowan Uni)
    Edith Cowan University
    Open grant
  • 2023 - 2026
    Adrenomedullin: a specific regulator of venous vessel integrity
    ARC Discovery Projects
    Open grant
  • 2022 - 2026
    Integrating innovative models of the brain microenvironment to identify new treatment strategies for medulloblastoma
    The Cure Starts Now Australia
    Open grant
  • 2022 - 2025
    Epithelial mechanics and the fate of cancer
    NHMRC IDEAS Grants
    Open grant
  • 2021 - 2025
    Blood Brain Barrier Program (Carrie Bickmore's Beanies 4 Brain Cancer Limited grant administered by The Walter and Eliza Hall Institute of Medical Research)
    Walter & Eliza Hall Institute of Medical Research (WEHI)
    Open grant

Past funding

  • 2023 - 2024
    Integrating innovative models of the brain microenvironment to identify new treatment strategies for medulloblastoma
    The Cure Starts Now Foundation
    Open grant
  • 2023
    IMB Stroke research led by Dr Anne Lagendijk
    Research Donation Generic
    Open grant
  • 2023
    Improving the accuracy of CCM modelling
    Be Brave for Life Foundation
    Open grant
  • 2020 - 2024
    Integrating innovative models of the brain microenvironment to identify new treatment strategies for medulloblastoma
    Brainchild Foundation
    Open grant
  • 2020
    Coupling the mechanical, signalling and transcriptional mechanisms that initiate pathogenesis of Cerebral Cavernous Malformation (NHMRC Project Grant administered by The University of Melbourne)
    University of Melbourne
    Open grant
  • 2020 - 2023
    Defining mechanisms behind the formation of hierarchical vascular networks
    ARC Discovery Projects
    Open grant
  • 2017 - 2020
    Coupling the mechanical, signalling and transcriptional mechanisms that initiate pathogenesis of Cerebral Cavernous Malformation
    NHMRC Project Grant
    Open grant
  • 2012 - 2015
    The contribution of cell-cell forces to vascular morphogenesis
    UQ Postdoctoral Research Fellowship
    Open grant

Supervision

Availability

Dr Anne Lagendijk is:
Available for supervision

Before you email them, read our advice on how to contact a supervisor.

Available projects

  • How to make and maintain a healthy vasculature

    Our group aims to understand how our blood vessel network is established during embryonic development and how its function is maintained during life. The development of an aberrant vessel network contributes to a range of cardio-vascular diseases. For example leaky vessels in the brain can cause stroke. In the lab we employ the zebrafish as a model organism since zebrafish embryos are viable ex utero and can be imaged at extremely high resolution which gives us a unique, live, view of vascular development. We make use of existing and novel biosensors in zebrafish to reveal dynamics of adhesion complexes at the cell-cell and cell-matrix interface whilst also examining distribution of tension at these mechanical hubs. By combining these high-end imaging approaches with detailed, innovative molecular genetics approaches, like CRISPR mutagenesis, we explore the fundamental importance of cell-matrix adhesion in vascular biology. We complement this in vivo modelling with analysis of bioengineered human micro-vessels. This adaptable system allows us to detail the impact of physical cues by the environment and by blood flow.

    PhD projects include:

    - The role of neuropeptides in establishing and maintaining blood vessels in zebrafish and 3D bioengineered human vessels.

    - Using CRISPR mutagenesis to uncover novel players that drive a vascular disease that can cause stroke.

    - Modelling how the brain vasculature impacts on brain tumors growth and cancer treatment using zebrafish models.

    - Profiling changes in tension across wild-type and mutant forms of the adhesion protein VE-cadherin using in vivo live imaging.

    - The impact of cell-matrix interactions on vascular growth in zebrafish mutant models.

Supervision history

Current supervision

  • Doctor Philosophy

    Investigating the role of the blood vasculature in Medulloblastoma progression using zebrafish models

    Principal Advisor

    Other advisors: Dr Laura Genovesi, Dr Emma Gordon

  • Doctor Philosophy

    Characterisation of a molecular pathway controlling cell-cell adhesion in veins but not arteries

    Principal Advisor

    Other advisors: Professor Alpha Yap

  • Doctor Philosophy

    Uncovering why CCM vascular malformations are restricted to the brain.

    Principal Advisor

    Other advisors: Professor Robert Parton

  • Doctor Philosophy

    Targetting blood vessel dysfunction in disease

    Principal Advisor

    Other advisors: Dr Samantha Stehbens, Dr Emma Gordon

  • Doctor Philosophy

    How the interaction between blood flow forces and ECM controls vessel assembly and function during development

    Associate Advisor

    Other advisors: Dr Mel White

  • Doctor Philosophy

    The role of c-Src in facilitating endothelial dysfunction in aortic disease

    Associate Advisor

    Other advisors: Dr Emma Gordon, Dr Lilian Schimmel

Completed supervision

Media

Enquiries

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communications@uq.edu.au