Overview
Availability
- Dr Anne Lagendijk is:
- Available for supervision
Qualifications
- Doctor of Philosophy, Utrecht University
Research interests
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Cellular mechanisms to maintain a healthy vasculature
Our vascular system transports approximately 7500 liters of blood each day. Arteries deliver oxygen and nutrients throughout the body after which the venous system returns the deoxygenated blood back to the heart. Architecturally, these blood vessels are extremely heterogeneous. The Lagendijk group investigates the development and maintenance of a functional blood vessel network in zebrafish and bioengineered human microvessels. The cells that make up our blood vessels continuously adapt their size, adhesiveness and compliance order to ensure the right balance between vessel integrity and permeability in a context dependent manner. Mechanical cues play a major role in the functional adaptation of blood vessels. Despite ongoing research unraveling the structural components of mechanical hubs in the cells, it is essential to assess the magnitude of forces that are transduced at these sites and the biological consequences for vessel function. Dr. Lagendijk has previously developed a VE-cadherin tension biosensor line in zebrafish. This line provides the first vertebrate model that reports intra-molecular tension and was utilised to identify changes in junctional organisation and VE-cadherin tension that occur as arteries mature and revealed molecular pathways that allow for this maturation to happen. In addition, the lab has established disease models for vascular malformations that are known to lead to neurological deficits and stroke. Modelling in zebrafish allows analyses of the initiating mechanisms of these vascular pathologies at unprecedented cellular and subcellular resolution.
Works
Search Professor Anne Lagendijk’s works on UQ eSpace
2024
Journal Article
c-Src induced vascular malformations require localised matrix degradation at focal adhesions
Essebier, Patricia, Keyser, Mikaela, Yordanov, Teodor, Hill, Brittany, Yu, Alexander, Noordstra, Ivar, Yap, Alpha S., Stehbens, Samantha J., Lagendijk, Anne K., Schimmel, Lilian and Gordon, Emma J. (2024). c-Src induced vascular malformations require localised matrix degradation at focal adhesions. Journal of Cell Science, 137 (13) jcs262101. doi: 10.1242/jcs.262101
2024
Journal Article
Hyaluronic acid turnover controls the severity of cerebral cavernous malformations in bioengineered human micro-vessels
Yordanov, Teodor E., Keyser, Mikaela S., Enriquez Martinez, Marco A., Esposito, Tyron, Tefft, Juliann B., Morris, Elysse K., Labzin, Larisa I., Stehbens, Samantha J., Rowan, Alan E., Hogan, Benjamin M., Chen, Christopher S., Lauko, Jan and Lagendijk, Anne K. (2024). Hyaluronic acid turnover controls the severity of cerebral cavernous malformations in bioengineered human micro-vessels. APL Bioengineering, 8 (1) 016108, 016108. doi: 10.1063/5.0159330
2023
Conference Publication
Uncovering Dynamic Changes In Medulloblastoma Associated Vasculature In Zebrafish
Morris, Elysse, Daignault-Mill, Sheena, Ju, Robert J., da Silva, Jason, Gordon, Emma, Wainwright, Brandon, Stehbens, Samantha, Genovesi, Laura and Lagendijk, Anne (2023). Uncovering Dynamic Changes In Medulloblastoma Associated Vasculature In Zebrafish. 2023 Pediatric Neuro-Oncology Research Conference, Washington, DC United States, 22-24 June 2023. Oxford, United Kingdom: Oxford University Press. doi: 10.1093/neuonc/noad073.274
2023
Journal Article
Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development
Grimm, Lin, Mason, Elizabeth, Yu, Hujun, Dudczig, Stefanie, Panara, Virginia, Chen, Tyrone, Bower, Neil I., Paterson, Scott, Rondon Galeano, Maria, Kobayashi, Sakurako, Senabouth, Anne, Lagendijk, Anne K., Powell, Joseph, Smith, Kelly A., Okuda, Kazuhide S., Koltowska, Katarzyna and Hogan, Benjamin M. (2023). Single‐cell analysis of lymphatic endothelial cell fate specification and differentiation during zebrafish development. The EMBO Journal, 42 (11) e112590, e112590. doi: 10.15252/embj.2022112590
2023
Journal Article
Macrophage ACE2 is necessary for SARS-CoV-2 replication and subsequent cytokine responses that restrict continued virion release
Labzin, Larisa I., Chew, Keng Yih, Eschke, Kathrin, Wang, Xiaohui, Esposito, Tyron, Stocks, Claudia J., Rae, James, Patrick, Ralph, Mostafavi, Helen, Hill, Brittany, Yordanov, Teodor E., Holley, Caroline L., Emming, Stefan, Fritzlar, Svenja, Mordant, Francesca L., Steinfort, Daniel P., Subbarao, Kanta, Nefzger, Christian M., Lagendijk, Anne K., Gordon, Emma J., Parton, Robert G., Short, Kirsty R., Londrigan, Sarah L. and Schroder, Kate (2023). Macrophage ACE2 is necessary for SARS-CoV-2 replication and subsequent cytokine responses that restrict continued virion release. Science Signaling, 16 (782) eabq1366, eabq1366. doi: 10.1126/scisignal.abq1366
2023
Journal Article
Addressing blood-brain-tumor-barrier heterogeneity in pediatric brain tumors with innovative preclinical models
Morris, Elysse K., Daignault-Mill, Sheena, Stehbens, Samantha J., Genovesi, Laura A. and Lagendijk, Anne K. (2023). Addressing blood-brain-tumor-barrier heterogeneity in pediatric brain tumors with innovative preclinical models. Frontiers in Oncology, 13 1101522, 1-9. doi: 10.3389/fonc.2023.1101522
2022
Journal Article
Dynamically regulated focal adhesions coordinate endothelial cell remodelling in developing vasculature
Chau, Tevin C. Y., Keyser, Mikaela S., Da Silva, Jason A., Morris, Elysse K., Yordanov, Teodor E., Duscyz, Kinga P., Paterson, Scott, Yap, Alpha S., Hogan, Benjamin M. and Lagendijk, Anne Karine (2022). Dynamically regulated focal adhesions coordinate endothelial cell remodelling in developing vasculature. Development, 149 (23) dev200454, 1-14. doi: 10.1242/dev.200454
2022
Journal Article
The people behind the papers – Tevin Chau and Anne Lagendijk
Lagendijk, Anne and Chau, Tevin (2022). The people behind the papers – Tevin Chau and Anne Lagendijk. Boletin de Malariologia y Salud Ambiental, 149 (23) dev201441. doi: 10.1242/dev.201441
2022
Journal Article
Editorial: Forces in biology - Cell and developmental mechanobiology and its implications in disease - Volume II
Wu, Selwin K., Gomez, Guillermo A., Stehbens, Samantha, Acharya, Bipul R., Ratheesh, Aparna, Priya, Rashmi, Lagendijk, Anne and Bershadsky, Alexander (2022). Editorial: Forces in biology - Cell and developmental mechanobiology and its implications in disease - Volume II. Frontiers in Cell and Developmental Biology, 10 1082857, 1082857. doi: 10.3389/fcell.2022.1082857
2022
Journal Article
Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection
Gibson, Josie F., Bojarczuk, Aleksandra, Evans, Robert J., Kamuyango, Alfred Alinafe, Hotham, Richard, Lagendijk, Anne K., Hogan, Benjamin M., Ingham, Philip W., Renshaw, Stephen A. and Johnston, Simon A. (2022). Blood vessel occlusion by Cryptococcus neoformans is a mechanism for haemorrhagic dissemination of infection. PLoS pathogens, 18 (4) e1010389, e1010389. doi: 10.1371/journal.ppat.1010389
2021
Journal Article
The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function
Koltowska, Katarzyna, Okuda, Kazuhide S., Gloger, Marleen, Rondon-Galeano, Maria, Mason, Elizabeth, Xuan, Jiachen, Dudczig, Stefanie, Chen, Huijun, Arnold, Hannah, Skoczylas, Renae, Bower, Neil I., Paterson, Scott, Lagendijk, Anne Karine, Baillie, Gregory J., Leshchiner, Ignaty, Simons, Cas, Smith, Kelly A., Goessling, Wolfram, Heath, Joan K., Pearson, Richard B., Sanij, Elaine, Schulte-Merker, Stefan and Hogan, Benjamin M. (2021). The RNA helicase Ddx21 controls Vegfc-driven developmental lymphangiogenesis by balancing endothelial cell ribosome biogenesis and p53 function. Nature Cell Biology, 23 (11), 1136-1147. doi: 10.1038/s41556-021-00784-w
2021
Journal Article
Endothelial cells are not productively infected by SARS‐CoV‐2
Schimmel, Lilian, Chew, Keng Yih, Stocks, Claudia J., Yordanov, Teodor E., Essebier, Patricia, Kulasinghe, Arutha, Monkman, James, Santos Miggiolaro, Anna Flavia Ribeiro, Cooper, Caroline, Noronha, Lucia, Schroder, Kate, Lagendijk, Anne Karine, Labzin, Larisa I., Short, Kirsty R. and Gordon, Emma J. (2021). Endothelial cells are not productively infected by SARS‐CoV‐2. Clinical and Translational Immunology, 10 (10) e1350, e1350. doi: 10.1002/cti2.1350
2021
Journal Article
Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice
Chau, Tevin CY., Baek, Sungmin, Coxam, Baptiste, Skoczylas, Renae, Rondon‐Galeano, Maria, Bower, Neil I., Wainwright, Elanor N., Stacker, Steven A., Cooper, Helen M., Koopman, Peter A., Lagendijk, Anne K., Harvey, Natasha L., François, Mathias and Hogan, Benjamin M. (2021). Pkd1 and Wnt5a genetically interact to control lymphatic vascular morphogenesis in mice. Developmental Dynamics, 251 (2) dvdy.390, 336-349. doi: 10.1002/dvdy.390
2021
Journal Article
Mechanotransduction activates RhoA in the neighbors of apoptotic epithelial cells to engage apical extrusion
Duszyc, Kinga, Gomez, Guillermo A., Lagendijk, Anne K., Yau, Mei-Kwan, Nanavati, Bageshri Naimish, Gliddon, Briony L., Hall, Thomas E., Verma, Suzie, Hogan, Benjamin M., Pitson, Stuart M., Fairlie, David P., Parton, Robert G. and Yap, Alpha S. (2021). Mechanotransduction activates RhoA in the neighbors of apoptotic epithelial cells to engage apical extrusion. Current Biology, 31 (6), 1326-1336.e5. doi: 10.1016/j.cub.2021.01.003
2020
Journal Article
Patient derived orthotopic xenograft models of Medulloblastoma lack a functional blood brain barrier
Genovesi, Laura A., Puttick, Simon, Millar, Amanda, Kojic, Marija, Ji, Pengxiang, Lagendijk, Anne K., Brighi, Caterina, Bonder, Claudine S, Adolphe, Christelle and Wainwright, Brandon J. (2020). Patient derived orthotopic xenograft models of Medulloblastoma lack a functional blood brain barrier. Neuro-Oncology, 23 (5), 732-742. doi: 10.1093/neuonc/noaa266
2020
Journal Article
Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion
Teo, Jessica L, Tomatis, Vanesa M, Coburn, Luke, Lagendijk, Anne K, Schouwenaar, Irin-Maya, Budnar, Srikanth, Hall, Thomas E, Verma, Suzie, McLachlan, Robert W, Hogan, Benjamin M, Parton, Robert G, Yap, Alpha S and Gomez, Guillermo A (2020). Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion. Molecular Biology of the Cell, 31 (23), 2495-2629. doi: 10.1091/mbc.E20-01-0084
2020
Journal Article
Localised Collagen2a1 secretion supports lymphatic endothelial cell migration in the zebrafish embryo
Chaudhury, Smrita, Okuda, Kazuhide S., Koltowska, Katarzyna, Lagendijk, Anne K., Paterson, Scott, Baillie, Gregory J., Simons, Cas, Smith, Kelly A., Hogan, Benjamin M. and Bower, Neil I. (2020). Localised Collagen2a1 secretion supports lymphatic endothelial cell migration in the zebrafish embryo. Development, 147 (18) dev190983, dev.190983. doi: 10.1242/dev.190983
2019
Journal Article
The alternative splicing regulator Nova2 constrains vascular Erk signaling to limit specification of the lymphatic lineage
Baek, Sungmin, Oh, Tae Gyu, Secker, Genevieve, Sutton, Drew L., Okuda, Kazuhide S., Paterson, Scott, Bower, Neil I., Toubia, John, Koltowska, Katarzyna, Capon, Samuel J., Baillie, Gregory J., Simons, Cas, Muscat, George E.O., Lagendijk, Anne K., Smith, Kelly A., Harvey, Natasha L. and Hogan, Benjamin M. (2019). The alternative splicing regulator Nova2 constrains vascular Erk signaling to limit specification of the lymphatic lineage. Developmental Cell, 49 (2), 279-292. doi: 10.1016/j.devcel.2019.03.017
2019
Journal Article
Peri-arterial specification of vascular mural cells from naive mesenchyme requires Notch signaling
Ando, Koji, Wang, Weili, Peng, Di, Chiba, Ayano, Lagendijk, Anne K., Barske, Lindsey, Crump, J. Gage, Stainier, Didier Y. R., Lendahl, Urban, Koltowska, Katarzyna, Hogan, Benjamin M., Fukuhara, Shigetomo, Mochizuki, Naoki and Betsholtz, Christer (2019). Peri-arterial specification of vascular mural cells from naive mesenchyme requires Notch signaling. Development, 146 (2) dev165589, dev165589. doi: 10.1242/dev.165589
2018
Journal Article
Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction
Paatero, Ilkka, Sauteur, Loïc, Lee, Minkyoung, Lagendijk, Anne K., Heutschi, Daniel, Wiesner, Cora, Guzmán, Camilo, Bieli, Dimitri, Hogan, Benjamin M., Affolter, Markus and Belting, Heinz-Georg (2018). Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature Communications, 9 (1) 3545, 3545. doi: 10.1038/s41467-018-05851-9
Funding
Current funding
Past funding
Supervision
Availability
- Dr Anne Lagendijk is:
- Available for supervision
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Available projects
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How to make and maintain a healthy vasculature
Our group aims to understand how our blood vessel network is established during embryonic development and how its function is maintained during life. The development of an aberrant vessel network contributes to a range of cardio-vascular diseases. For example leaky vessels in the brain can cause stroke. In the lab we employ the zebrafish as a model organism since zebrafish embryos are viable ex utero and can be imaged at extremely high resolution which gives us a unique, live, view of vascular development. We make use of existing and novel biosensors in zebrafish to reveal dynamics of adhesion complexes at the cell-cell and cell-matrix interface whilst also examining distribution of tension at these mechanical hubs. By combining these high-end imaging approaches with detailed, innovative molecular genetics approaches, like CRISPR mutagenesis, we explore the fundamental importance of cell-matrix adhesion in vascular biology. We complement this in vivo modelling with analysis of bioengineered human micro-vessels. This adaptable system allows us to detail the impact of physical cues by the environment and by blood flow.
PhD projects include:
- The role of neuropeptides in establishing and maintaining blood vessels in zebrafish and 3D bioengineered human vessels.
- Using CRISPR mutagenesis to uncover novel players that drive a vascular disease that can cause stroke.
- Modelling how the brain vasculature impacts on brain tumors growth and cancer treatment using zebrafish models.
- Profiling changes in tension across wild-type and mutant forms of the adhesion protein VE-cadherin using in vivo live imaging.
- The impact of cell-matrix interactions on vascular growth in zebrafish mutant models.
Supervision history
Current supervision
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Doctor Philosophy
Investigating the role of the blood vasculature in Medulloblastoma progression using zebrafish models
Principal Advisor
Other advisors: Dr Laura Genovesi, Dr Emma Gordon
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Doctor Philosophy
Characterisation of a molecular pathway controlling cell-cell adhesion in veins but not arteries
Principal Advisor
Other advisors: Professor Alpha Yap
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Doctor Philosophy
Uncovering why CCM vascular malformations are restricted to the brain.
Principal Advisor
Other advisors: Professor Robert Parton
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Doctor Philosophy
Targetting blood vessel dysfunction in disease
Principal Advisor
Other advisors: Dr Samantha Stehbens, Dr Emma Gordon
-
Doctor Philosophy
How the interaction between blood flow forces and ECM controls vessel assembly and function during development
Associate Advisor
Other advisors: Dr Mel White
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Doctor Philosophy
The role of c-Src in facilitating endothelial dysfunction in aortic disease
Associate Advisor
Other advisors: Dr Emma Gordon, Dr Lilian Schimmel
Completed supervision
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2021
Doctor Philosophy
Cell Polarity and Cell-Matrix Adhesion in Vascular Development
Principal Advisor
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2020
Doctor Philosophy
Defining the early molecular events governing AVC formation in zebrafish
Associate Advisor
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2019
Doctor Philosophy
Elimination of apoptotic epithelial cells: Regulation of apoptotic extrusion and immune responses to epithelial apoptosis
Associate Advisor
Other advisors: Professor Alpha Yap
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2018
Doctor Philosophy
Characterisation of novel regulators of cardiovascular development in zebrafish
Associate Advisor
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2017
Doctor Philosophy
Characterisation of novel molecular mechanisms of lymphatic vascular development
Associate Advisor
Media
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