ASAP-NMR: A leap forward in structural studies of proteins using NMR spectroscopy (2012-2016)
Abstract
Nuclear magnetic resonance (NMR) is the only method that enables study of the structure of proteins in solution at atomic resolution. However, its use has been limited by its low throughput relative to X-ray crystallography. I have developed substantially faster methods for NMR data acquisition and I now propose to fully automate the protein structure determination process using NMR so that solution structures can be determined in less than 1 week. I will apply my method to study disulfide-rich proteins isolated from animal venoms, a proven source of drug and insecticide leads for which limited structural data is available. This will yield novel 3D structures with unprecedented precision and allow the engineering of new bioactive peptides.
