Overview
Background
1991-94 BSc Honours I (Chemistry) University of Queensland, University Medal
1995-99 PhD (Cancer Pathology) University of Queensland
1999-00 Post-Doctoral Fellow, Queensland University of Technology
2001-03 NHMRC CJ Martin/RG Menzies Fellow, Scripps Research Institute, San Diego, CA, USA
2003-05 NHMRC CJ Martin/RG Menzies Fellow, Queensland University of Technology
2005-09 NHMRC RD Wright Fellow, Queensland University of Technology
2010-15 Associate Professor, Mater Research Institute, The University of Queensland
2012-16 ARC Future Fellow, Mater Research Institute, The University of Queensland
2016- Professor of Cancer Biology, Mater Research Institute, The University of Queensland
Availability
- Honorary Professor John Hooper is:
- Available for supervision
Research interests
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Cancers of the reproductive system and gastrointestinal tract
Our focus is on the identification and evaluation of molecular targets and biomarkers of cancer. As much as possible our research employs disease relevant models that incorporate patient tumours. We have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals.
Research impacts
My major research contributions are in the identification and evaluation of molecular targets and biomarkers for cancers of the ovary, pancreas, prostate and bowel. At a molecular level my focus is on cell surface receptors, proteolytic enzymes, intracellular signal transducers, mediators of metabolism and protein post-translational modifications. Most recently we have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals. To date my research has attracted ~$11M in funding, producing 4 patents and 95 papers.
Works
Search Professor John Hooper’s works on UQ eSpace
2003
Journal Article
Mouse matriptase-2: identification, characterization and comparative mRNA expression analysis with mouse hepsin in adult and embryonic tissues
Hooper, John D., Campagnolo, Luisa, Goodarzi, Goodarz, Truong, Tony N., Stuhlmann, Heidi and Quigley, James P. (2003). Mouse matriptase-2: identification, characterization and comparative mRNA expression analysis with mouse hepsin in adult and embryonic tissues. Biochemical Journal, 373 (3), 689-702. doi: 10.1042/BJ20030390
2003
Journal Article
Membrane anchored serine proteases: A rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer
Netzel-Arnett, Sarah, Hooper, John D., Szabo, Roman, Madison, Edwin L., Quigley, James P., Bugge, Thomas H. and Antalis, Toni M. (2003). Membrane anchored serine proteases: A rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer. Cancer and Metastasis Reviews, 22 (2-3), 237-258. doi: 10.1023/A:1023003616848
2003
Journal Article
Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen
Hooper, John D., Zijlstra, Andries, Aimes, Ronald T., Liang, Hongyan, Claassen, Gisela F., Tarin, David, Testa, Jacqueline E. and Quigley, James P. (2003). Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene, 22 (12), 1783-1794. doi: 10.1038/sj.onc.1206220
2003
Journal Article
Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis
Aimes, RT, Zijlstra, A, Hooper, JD, Ogbourne, SM, Sit, ML, Fuchs, S, Gotley, DC, Quigley, JP and Antalis, TM (2003). Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thrombosis And Haemostasis, 89 (3), 561-572. doi: 10.1055/s-0037-1613388
2002
Journal Article
A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction
Zijlstra, A, Mellor, R, Panzarella, G, Aimes, RT, Hooper, JD, Marchenko, ND and Quigley, JP (2002). A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction. Cancer Research, 62 (23), 7083-7092.
2001
Journal Article
TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is located on 17q24 and upregulated in renal cell carcinoma
Rae, Fiona K., Hooper, John D., Eyre, Helen J., Sutherland, Grant R., Nicol, David L. and Clements, Judith A. (2001). TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is located on 17q24 and upregulated in renal cell carcinoma. Genomics, 77 (3), 200-207. doi: 10.1006/geno.2001.6629
2001
Journal Article
Identification and characterization of KLK14, a novel kallikrein serine protease gene located on human chromosome 19q13.4 and expressed in prostate and skeletal muscle
Hooper, John D., Bui, Loan T., Rae, Fiona K., Harvey, Tracey J., Myers, Stephen A., Ashworth, Linda K. and Clements, Judith A. (2001). Identification and characterization of KLK14, a novel kallikrein serine protease gene located on human chromosome 19q13.4 and expressed in prostate and skeletal muscle. Genomics, 73 (1), 117-122. doi: 10.1006/geno.2000.6490
2001
Journal Article
Organization and chromosomal localization of the murine Testisin gene encoding a serine protease temporally expressed during spermatogenesis
Scarman, Anthony L., Hooper, John D., Boucaut, Kerry J., Sit, Mae-Le, Webb, Graham C., Normyle, John F. and Antalis, Toni M. (2001). Organization and chromosomal localization of the murine Testisin gene encoding a serine protease temporally expressed during spermatogenesis. European Journal of Biochemistry, 268 (5), 1250-1258. doi: 10.1046/j.1432-1327.2001.01986.x
2001
Journal Article
Type II transmembrane serine proteases - Insights into an emerging class of cell surface proteolytic enzymes
Hooper, John D., Clements, Judith A., Quigley, James P. and Antalis, Toni M. (2001). Type II transmembrane serine proteases - Insights into an emerging class of cell surface proteolytic enzymes. Journal of Biological Chemistry, 276 (2), 857-860. doi: 10.1074/jbc.R000020200
2001
Conference Publication
Identification and characterization of a novel gene STAG1 is up-regulated in renal cell carcinoma
Rae, F., Hooper, J.D., Nicol, D. L. and Clements, J.A. (2001). Identification and characterization of a novel gene STAG1 is up-regulated in renal cell carcinoma. 2001 Annual Scientific Meeting of the Urological Society of Australasia, Coolum, Australia, March 2001. Australia: Blackwell Publishing Asia.
2001
Journal Article
The expanded human kallikrein (KLK) gene family: genomic organisation, tissue-specific expression and potential function.
Clements, Judith, Hooper, John, Dong, Ying and Harvey, Tracey (2001). The expanded human kallikrein (KLK) gene family: genomic organisation, tissue-specific expression and potential function.. Biological Chemistry, 382 (1), 5-14. doi: 10.1515/BC.2001.002
2000
Journal Article
Tissue-specific expression patterns and fine mapping of the human kallikrein (KLK) locus on proximal 19q13.4
Harvey, TJ, Hooper, JD, Myers, SA, Stephenson, SA, Ashworth, LK and Clements, JA (2000). Tissue-specific expression patterns and fine mapping of the human kallikrein (KLK) locus on proximal 19q13.4. Journal of Biological Chemistry, 275 (48), 37397-37406. doi: 10.1074/jbc.M004525200
2000
Journal Article
Cloning of the cDNA and localization of the gene encoding human NRBP, a ubiquitously expressed, multidomain putative adapter protein
Hooper, John D., Baker, Elizabeth, Ogbourne, Steven M., Sutherland, Grant R. and Antalis, Toni M. (2000). Cloning of the cDNA and localization of the gene encoding human NRBP, a ubiquitously expressed, multidomain putative adapter protein. Genomics, 66 (1), 113-118. doi: 10.1006/geno.2000.6167
2000
Conference Publication
Localization, structure and regulation of the human PRSS14 gene encoding the serine proteinase Testisin
Antalis, T. M., Boucaut, K. J., Normyle, J. F., Fitzpatrick, D. R. and Hooper, J. (2000). Localization, structure and regulation of the human PRSS14 gene encoding the serine proteinase Testisin. 91st Annual Meeting of the American Association for Cancer Research, San Francisco, 1-5 Apr, 2000. Philadelphia, U.S.A.: American Association for Cancer Research.
2000
Journal Article
Localization, expression and genomic structure of the gene encoding the human serine protease testisin
Hooper, John D., Bowen, Natalie, Marshall, Heidi, Cullen, Lara M., Sood, Raman, Daniels, Rachael, Stuttgen, Melanie A., Normyle, John F., Higgs, Douglas R., Kastner, Daniel L., Ogbourne, Steven M., Pera, Martin F., Jazwinska, Elizabeth C. and Antalis, Toni M. (2000). Localization, expression and genomic structure of the gene encoding the human serine protease testisin. Biochimica et Biophysica Acta, 1492 (1), 63-71. doi: 10.1016/S0167-4781(00)00071-3
2000
Journal Article
Localization of the mosaic transmembrane serine protease corin to heart myocytes
Hooper, J. D., Scarman, A. L., Clarke, B., Normyle, J. F. and Antalis, T. M. (2000). Localization of the mosaic transmembrane serine protease corin to heart myocytes. European Journal of Biochemistry, 267 (23), 6931-6937. doi: 10.1046/j.1432-1327.2000.01806.x
1999
Journal Article
Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors
Hooper, J. D., Nicol, D. L., Dickinson, J. L., Eyre, H. J., Scarman, A. L., Normyle, J. F., Stuttgen, M. A., Douglas, M. L., Loveland, K. A. L., Sutherland, G. R. and Antalis, T. M. (1999). Testisin, a new human serine proteinase expressed by premeiotic testicular germ cells and lost in testicular germ cell tumors. Cancer Research, 59 (13), 3199-3205.
Funding
Current funding
Supervision
Availability
- Honorary Professor John Hooper is:
- Available for supervision
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Supervision history
Current supervision
-
Doctor Philosophy
Molecular and cellular determinants of CDCP1 targeted, payload-delivery antibodies.
Principal Advisor
Other advisors: Associate Professor Michael Landsberg
-
Doctor Philosophy
Understanding the function of CDCP1 and its potential as a theranostic target for cholangiocarcinoma
Principal Advisor
Other advisors: Professor Kristofer Thurecht
-
Doctor Philosophy
Investigations into genetic aberrations underlying recurrent ovarian cancer
Principal Advisor
Other advisors: Professor Geoff Faulkner
-
Doctor Philosophy
Novel theranostic targets for metastatic colorectal cancer
Principal Advisor
Other advisors: Professor David Clark
-
Doctor Philosophy
Cancer-associated post-translational modifications of the receptor CDCP1 Background:
Principal Advisor
-
Doctor Philosophy
Factors impacting receptor processing in response to peptide and antibody ligands
Principal Advisor
Other advisors: Dr Jodi Saunus
-
Doctor Philosophy
Characterisation of EV-associated lipids in the progression of ovarian cancer
Associate Advisor
Other advisors: Dr Dominic Guanzon, Professor Carlos Salomon Gallo, Dr Andrew Lai
-
Doctor Philosophy
Developing new strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
-
Doctor Philosophy
Developing novel strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
-
Doctor Philosophy
Enhancing immune responses to cancer
Associate Advisor
Other advisors: Dr Jazmina Gonzalez Cruz, Professor Brian Gabrielli
-
Doctor Philosophy
Biomarker driven diagnostic and therapeutic innovations in breast cancer
Associate Advisor
-
Doctor Philosophy
Genomic and epigenomic correlates of prostate cancer therapy
Associate Advisor
Other advisors: Associate Professor Adam Ewing
Completed supervision
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2022
Doctor Philosophy
Profiling of Immunoglobulin (Ig) G, IgM and IgA Isotype Immune Responses and Development of Autoantibody Biomarkers for Early Detection of Colorectal Cancer
Principal Advisor
-
2022
Doctor Philosophy
Theranostics: Molecular Imaging and Molecularly-Directed Radionuclide Therapy for Metastatic Colorectal Cancer
Principal Advisor
-
2022
Doctor Philosophy
Investigating New Therapeutic Targets for Clear Cell Cancers
Principal Advisor
-
2016
Doctor Philosophy
Thesis Title: Investigation into the role of the cell surface glycoprotein CDCP1 in high-grade serous ovarian cancer progression
Principal Advisor
Other advisors: Professor Brian Gabrielli
-
2020
Doctor Philosophy
Opportunities for epigenetic therapies in ovarian cancer
Associate Advisor
Other advisors: Associate Professor Jason Lee
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2020
Doctor Philosophy
Regulation of Epithelial Ovarian Cancer Initiation and Progression by Exosomal Proteins and miRNAs
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2020
Doctor Philosophy
New Strategies for Identification of Therapeutic Target of Ovarian Cancer
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2018
Master Philosophy
The effects of Rab13 derived from lymph node stromal cell extracellular vesicles on the pathogenesis of colorectal cancer
Associate Advisor
-
2018
Doctor Philosophy
Dysregulation of EGF-trafficking in squamous cell carcinoma and cetuximab resistance
Associate Advisor
Other advisors: Associate Professor Fiona Simpson
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2018
Doctor Philosophy
Targeting Apoptosis as A Novel Therapy for Medulloblastoma
Associate Advisor
Other advisors: Dr Laura Genovesi, Professor Brandon Wainwright
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2010
Doctor Philosophy
Androgen receptor, caveolin-1 and androgen self-sufficiency in prostate cancer
Associate Advisor
Other advisors: Professor David Johnson
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