
Overview
Background
1991-94 BSc Honours I (Chemistry) University of Queensland, University Medal
1995-99 PhD (Cancer Pathology) University of Queensland
1999-00 Post-Doctoral Fellow, Queensland University of Technology
2001-03 NHMRC CJ Martin/RG Menzies Fellow, Scripps Research Institute, San Diego, CA, USA
2003-05 NHMRC CJ Martin/RG Menzies Fellow, Queensland University of Technology
2005-09 NHMRC RD Wright Fellow, Queensland University of Technology
2010-15 Associate Professor, Mater Research Institute, The University of Queensland
2012-16 ARC Future Fellow, Mater Research Institute, The University of Queensland
2016- Professor of Cancer Biology, Mater Research Institute, The University of Queensland
Availability
- Honorary Professor John Hooper is:
- Available for supervision
- Media expert
Fields of research
Research interests
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Cancers of the urological system, gynaecological system and gastrointestinal tract
Our focus is on the identification and evaluation of molecular targets and biomarkers of cancer. As much as possible our research employs disease relevant models that incorporate patient tumours. We have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in a PET-CT imaging clinical trial evaluating a new radio-imaging agent to guide targeted therapy for ovarian and bladder cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals.
Research impacts
My major research contributions are in the identification and evaluation of molecular targets and biomarkers for cancers of the ovary, pancreas, prostate and bowel. At a molecular level my focus is on cell surface receptors, proteolytic enzymes, intracellular signal transducers, mediators of metabolism and protein post-translational modifications. Most recently we have developed a successful R&D pipeline to identify cell surface receptors that are enriched in cancer for the purpose of targeting them for delivery of radiation and cytotoxins for cancer detection and treatment. This has culminated in phase 1 PET-CT imaging clinical trials evaluating the safety and tumour/normal biodistribution of a new radio-imaging agent to guide targeted therapy for ovarian and bladder cancer. My team is expert in generating and employing in vitro, ex vivo and mouse models of cancer, using patient specimens for much of this work. We have extensive experience in cell and molecular biology, protein analysis, including generation, purification and characterisation of recombinant proteins from insect and mammalian cells, enzymology, wide field fluorescent and confocal microscopy of live and fixed specimens, flow cytometry analysis and fluorescent activated cell sorting, bioluminescent and PET/CT imaging of mouse models of cancer, and histological and immunohistochemical analysis of mouse xenografts and patient tumours. We also have expertise in radio- and cytotoxin-labelling of biomolecules using these for detection and treatment of cancer in preclinical models. Our discovery and translational research activities are supported by close collaborations with medical specialists involved in treatment and diagnosis of cancer at Mater, Royal Brisbane and Women’s, Wesley, and Princess Alexandra Hospitals. To date my research has attracted ~$17M in funding, producing 4 patents and 128 papers.
Works
Search Professor John Hooper’s works on UQ eSpace
2009
Journal Article
Functional role of cell surface CUB domain-containing protein 1 in tumor cell dissemination
Deryugina, Elena I., Conn, Erin M., Wortmann, Andreas, Partridge, Juneth J., Kupriyanova, Tatyana A., Ardi, Veronica C., Hooper, John D. and Quigley, James P. (2009). Functional role of cell surface CUB domain-containing protein 1 in tumor cell dissemination. Molecular Cancer Research, 7 (8), 1197-1211. doi: 10.1158/1541-7786.MCR-09-0100
2009
Journal Article
Matriptase-2 (TMPRSS6): A proteolytic regulator of iron homeostasis
Ramsay, Andrew J., Hooper, John D., Folgueras, Alicia R., Velasco, Gloria and Lopez-Otin, Carlos (2009). Matriptase-2 (TMPRSS6): A proteolytic regulator of iron homeostasis. Haematologica, 94 (6), 840-849. doi: 10.3324/haematol.2008.001867
2009
Journal Article
The cell surface glycoprotein CDCP1 in cancer - Insights, opportunities, and challenges
Wortmann, Andreas, He, Yaowu, Deryugina, Elena I., Quigley, James P. and Hooper, John D. (2009). The cell surface glycoprotein CDCP1 in cancer - Insights, opportunities, and challenges. IUBMB Life, 61 (7), 723-730. doi: 10.1002/iub.198
2009
Conference Publication
Kallikrein-related proteases as novel therapeutic targets in prostate and ovarian cancer
Clements, J. A., Dong, Y., Loessner, D., Tan, O., Sieh, S., Reichert, J., Burke, L., Stephens, C., Lawrence, M., Stansfield, S., Swedberg, J., Ramsay, A., Hooper, J., Harris, J. and Hutmacher, D. (2009). Kallikrein-related proteases as novel therapeutic targets in prostate and ovarian cancer. 40th Annual Conference of the Society for Reproductive Biology, Clayton, VIC, Australia: CSIRO Publishing. doi: 10.1071/srb09abs009
2008
Journal Article
The ubiquitin-protein ligase Nedd4-2 differentially interacts with and regulates members of the Tweety family of chloride ion channels
He, Yaowu, Hryciw, Deanne H., Carroll, Melanie L., Myers, Stephen A., Whitbread, Astrid K., Kumar, Sharad, Poronnik, Philip and Hooper, John D. (2008). The ubiquitin-protein ligase Nedd4-2 differentially interacts with and regulates members of the Tweety family of chloride ion channels. Journal of Biological Chemistry, 283 (35), 24000-24010. doi: 10.1074/jbc.M803361200
2008
Journal Article
N-glycosylation analysis of the human Tweety family of putative chloride ion channels supports a penta-spanning membrane arrangement: impact of N-glycosylation on cellular processing of Tweety homologue 2 (TTYH2)
He, Yaowu, Ramsay, Andrew, Hunt, Melanie, Whitbread, Astrid, Myers, Stephen and Hooper, John (2008). N-glycosylation analysis of the human Tweety family of putative chloride ion channels supports a penta-spanning membrane arrangement: impact of N-glycosylation on cellular processing of Tweety homologue 2 (TTYH2). Biochemical Journal, 412 (1), 45-55. doi: 10.1042/BJ20071722
2008
Journal Article
Kallikrein-related peptidase 4( KLK4)initiates intracellular signaling via protease-activated receptors(PARs):KLK4 and PAR-2 are co-expressed during prostate cancer progression
Ramsay, Andrew J., Dong, Ying, Hunt, Melanie L., Linn, MayLa, Samaratunga, Hemamali, Clements, Judith A. and Hooper, John D. (2008). Kallikrein-related peptidase 4( KLK4)initiates intracellular signaling via protease-activated receptors(PARs):KLK4 and PAR-2 are co-expressed during prostate cancer progression. The Journal of Biological Chemistry, 283 (18), 12293-12304. doi: 10.1074/jbc.M709493200
2008
Journal Article
A novel transcript from the KLKP1 gene is androgen regulated, down-regulated during prostate cancer progression and encodes the first non-serine protease identified from the human kallikrein gene locus
Myers, Stephen A., Kaushal, Aneel, Dong, Ying, Lai, John, Tan, Olivia L., Bui, Loan T., Hunt, Melanie L., Digby, Matthew R., Samaratunga, Hemamali, Gardiner, Robert A., Clements, Judith A. and Hooper, John D. (2008). A novel transcript from the KLKP1 gene is androgen regulated, down-regulated during prostate cancer progression and encodes the first non-serine protease identified from the human kallikrein gene locus. Prostate, 68 (4), 381-399. doi: 10.1002/pros.20685
2008
Journal Article
Tissue-specific promoter utilisation of the kallikrein-related peptidase genes, KLK5 and KLK7, and cellular localisation of the encoded proteins suggest roles in exocrine pancreatic function
Dong, Ying, Matigian, Nick, Harvey, Tracey J., Samaratunga, Hemamali, Hooper, John D. and Clements, Judith A. (2008). Tissue-specific promoter utilisation of the kallikrein-related peptidase genes, KLK5 and KLK7, and cellular localisation of the encoded proteins suggest roles in exocrine pancreatic function. Biological Chemistry, 389 (2), 99-109. doi: 10.1515/BC.2008.013
2008
Journal Article
The type II transmembrane serine protease Matriptase-2 - identification, structural features, enzymology, expression pattern and potential roles
Ramsay, Andrew J., Reid, Janet C., Velasco, Gloria, Quigley, James P. and Hooper, John D. (2008). The type II transmembrane serine protease Matriptase-2 - identification, structural features, enzymology, expression pattern and potential roles. Frontiers in Bioscience, 13 (2), 569-579. doi: 10.2741/2702
2008
Conference Publication
Prostatic trypsin-like kallikrein-related peptidases (KLKs) and other prostate-expressed tryptic proteinases as regulators of signalling via proteinase-activated receptors (PARs)
Ramsay, Andrew J., Reid, Janet C., Adams, Mark N., Samaratunga, Hemamali, Dong, Ying, Clements, Judith A. and Hooper, John D. (2008). Prostatic trypsin-like kallikrein-related peptidases (KLKs) and other prostate-expressed tryptic proteinases as regulators of signalling via proteinase-activated receptors (PARs). 2nd International Symposium on Kallikrein and Kallikrein-Related Peptidases (ISK 2007), Santorini, Greece, 16-18 October 2007. Berlin, Germany: Walter de Gruyter. doi: 10.1515/BC.2008.078
2007
Journal Article
Potential physiological and pathophysiological roles for protease-activated receptor-2 in the kidney
Vesey, David A., Hooper, John D., Gobe, Glenda C. and Johnson, David W. (2007). Potential physiological and pathophysiological roles for protease-activated receptor-2 in the kidney. Nephrology, 12 (1), 36-43. doi: 10.1111/j.1440-1797.2006.00746.x
2005
Journal Article
The membrane-anchored serine protease, TMPRSS2, activates PAR-2 in prostate cancer cells
Wilson, S, Greer, B, Hooper, J, Zijlstra, A, Walker, B, Quigley, J and Hawthorne, S (2005). The membrane-anchored serine protease, TMPRSS2, activates PAR-2 in prostate cancer cells. Biochemical Journal, 388 (3), 967-972. doi: 10.1042/BJ20041066
2004
Book Chapter
The tissue kallikrein gene cluster
Clements, J. A., Hooper, John David, Odorico, D. M. and Dong, Y. (2004). The tissue kallikrein gene cluster. Handbook of Proteolytic Enzymes / 1. Aspartic and metallo peptidases. (pp. 478-478) edited by Barrett, A. J., Rawlings, N. D. and Woessner, J. F.. Amsterdam, The Netherlands: Elsevier Academic Press.
2003
Journal Article
Mouse matriptase-2: identification, characterization and comparative mRNA expression analysis with mouse hepsin in adult and embryonic tissues
Hooper, John D., Campagnolo, Luisa, Goodarzi, Goodarz, Truong, Tony N., Stuhlmann, Heidi and Quigley, James P. (2003). Mouse matriptase-2: identification, characterization and comparative mRNA expression analysis with mouse hepsin in adult and embryonic tissues. Biochemical Journal, 373 (3), 689-702. doi: 10.1042/BJ20030390
2003
Journal Article
Membrane anchored serine proteases: A rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer
Netzel-Arnett, Sarah, Hooper, John D., Szabo, Roman, Madison, Edwin L., Quigley, James P., Bugge, Thomas H. and Antalis, Toni M. (2003). Membrane anchored serine proteases: A rapidly expanding group of cell surface proteolytic enzymes with potential roles in cancer. Cancer and Metastasis Reviews, 22 (2-3), 237-258. doi: 10.1023/A:1023003616848
2003
Journal Article
Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen
Hooper, John D., Zijlstra, Andries, Aimes, Ronald T., Liang, Hongyan, Claassen, Gisela F., Tarin, David, Testa, Jacqueline E. and Quigley, James P. (2003). Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen. Oncogene, 22 (12), 1783-1794. doi: 10.1038/sj.onc.1206220
2003
Journal Article
Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis
Aimes, RT, Zijlstra, A, Hooper, JD, Ogbourne, SM, Sit, ML, Fuchs, S, Gotley, DC, Quigley, JP and Antalis, TM (2003). Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thrombosis And Haemostasis, 89 (3), 561-572. doi: 10.1055/s-0037-1613388
2002
Journal Article
A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction
Zijlstra, A, Mellor, R, Panzarella, G, Aimes, RT, Hooper, JD, Marchenko, ND and Quigley, JP (2002). A quantitative analysis of rate-limiting steps in the metastatic cascade using human-specific real-time polymerase chain reaction. Cancer Research, 62 (23), 7083-7092.
2001
Journal Article
TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is located on 17q24 and upregulated in renal cell carcinoma
Rae, Fiona K., Hooper, John D., Eyre, Helen J., Sutherland, Grant R., Nicol, David L. and Clements, Judith A. (2001). TTYH2, a human homologue of the Drosophila melanogaster gene tweety, is located on 17q24 and upregulated in renal cell carcinoma. Genomics, 77 (3), 200-207. doi: 10.1006/geno.2001.6629
Funding
Current funding
Supervision
Availability
- Honorary Professor John Hooper is:
- Available for supervision
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Available projects
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Cellular targets for cancer detection and treatment
The project involves the use of state-of-the-art in silico and omics approaches to identify antigens that are suitable targets for delivery of radioactive and cytotoxic payloads to cancers. Candidates will be validated by analysis of patient tumours and normal organs.
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Agents for targeted delivery of cytotoxins to cancer
A range of screening approaches will be employed to identify organic compounds, peptides and antibodies that bind with high affinity and specificity to antigens enriched on the surface of cancer cells. The efficacy of these agents for delivery of payloads to cancer will be evaluated using cellular and mouse models of cancer.
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Disrupting metabolsim to improve cancer treatment efficacy
The project will employ disease-relevant in vitro mouse models to test metabolism modulating approaches to improve the efficacy of current anti-cancer treatments.
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Targeting cell division to significatly improve the effectiveness of ovarian cancer treatments
The project will employ nanoparticle formulations of cell division disrupting drugs against patient-derived in vitro, ex vivo and in vivo models of high-grade serous ovarian cancer.
Supervision history
Current supervision
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Doctor Philosophy
Understanding the function of CDCP1 and its potential as a theranostic target for cholangiocarcinoma
Principal Advisor
Other advisors: Professor Kristofer Thurecht
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Doctor Philosophy
Cancer-associated post-translational modifications of the receptor CDCP1 Background:
Principal Advisor
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Doctor Philosophy
Novel Theranostic Targets for Colorectal Cancer
Principal Advisor
Other advisors: Professor David Clark
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Doctor Philosophy
Factors impacting receptor processing in response to peptide and antibody ligands
Principal Advisor
Other advisors: Dr Justin Goh
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Doctor Philosophy
Dual antibody targeting of CDCP1 for Breast and Ovarian cancers
Principal Advisor
Other advisors: Associate Professor Michael Landsberg
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Doctor Philosophy
Development of antibody-drug conjugates against hard-to-cure solid cancers
Associate Advisor
Other advisors: Dr Brett Paterson, Associate Professor Fernando Guimaraes
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Doctor Philosophy
Developing new strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
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Doctor Philosophy
Developing novel strategies to overcome immune suppression in cancer
Associate Advisor
Other advisors: Dr Sherry Wu
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Doctor Philosophy
Enhancing immune responses to cancer
Associate Advisor
Other advisors: Dr Jazmina Gonzalez Cruz, Professor Brian Gabrielli
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Doctor Philosophy
Genomic and epigenomic correlates of prostate cancer therapy
Associate Advisor
Other advisors: Associate Professor Adam Ewing
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Doctor Philosophy
Characterisation of EV-associated lipids in the progression of ovarian cancer
Associate Advisor
Other advisors: Dr Dominic Guanzon, Professor Carlos Salomon Gallo, Dr Andrew Lai
Completed supervision
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2022
Doctor Philosophy
Profiling of Immunoglobulin (Ig) G, IgM and IgA Isotype Immune Responses and Development of Autoantibody Biomarkers for Early Detection of Colorectal Cancer
Principal Advisor
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2022
Doctor Philosophy
Theranostics: Molecular Imaging and Molecularly-Directed Radionuclide Therapy for Metastatic Colorectal Cancer
Principal Advisor
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2022
Doctor Philosophy
Investigating New Therapeutic Targets for Clear Cell Cancers
Principal Advisor
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2016
Doctor Philosophy
Thesis Title: Investigation into the role of the cell surface glycoprotein CDCP1 in high-grade serous ovarian cancer progression
Principal Advisor
Other advisors: Professor Brian Gabrielli
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2025
Doctor Philosophy
Biomarker driven diagnostic and therapeutic innovations in breast cancer
Associate Advisor
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2020
Doctor Philosophy
Opportunities for epigenetic therapies in ovarian cancer
Associate Advisor
Other advisors: Associate Professor Jason Lee
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2020
Doctor Philosophy
Regulation of Epithelial Ovarian Cancer Initiation and Progression by Exosomal Proteins and miRNAs
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2020
Doctor Philosophy
New Strategies for Identification of Therapeutic Target of Ovarian Cancer
Associate Advisor
Other advisors: Professor Carlos Salomon Gallo
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2018
Master Philosophy
The effects of Rab13 derived from lymph node stromal cell extracellular vesicles on the pathogenesis of colorectal cancer
Associate Advisor
Other advisors: Honorary Professor Li Li
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2018
Doctor Philosophy
Dysregulation of EGF-trafficking in squamous cell carcinoma and cetuximab resistance
Associate Advisor
Other advisors: Professor Fiona Simpson
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2018
Doctor Philosophy
Targeting Apoptosis as A Novel Therapy for Medulloblastoma
Associate Advisor
Other advisors: Dr Laura Genovesi, Professor Brandon Wainwright
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2010
Doctor Philosophy
Androgen receptor, caveolin-1 and androgen self-sufficiency in prostate cancer
Associate Advisor
Other advisors: Professor David Johnson
Media
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