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2025 Journal Article Structural basis for TIR domain–mediated innate immune signaling by Toll-like receptor adaptors TRIF and TRAMManik, Mohammad K., Pan, Mengqi, Xiao, Le, Gu, Weixi, Kim, Hyoyoung, Pospich, Sabrina, Hedger, Andrew, Vajjhala, Parimala R., Lee, Morris Y. L., Qian, Xiaoqi, Landsberg, Michael J., Ve, Thomas, Nanson, Jeffrey D., Raunser, Stefan, Stacey, Katryn J., Wu, Hao and Kobe, Bostjan (2025). Structural basis for TIR domain–mediated innate immune signaling by Toll-like receptor adaptors TRIF and TRAM. Proceedings of the National Academy of Sciences, 122 (2) e2418988122, e2418988122-2. doi: 10.1073/pnas.2418988122 |
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2024 Journal Article o-Vanillin binds covalently to MAL/TIRAP Lys-210 but independently inhibits TLR2Rahaman, Md. Habibur, Thygesen, Sara J., Maxwell, Michael J., Kim, Hyoyoung, Mudai, Prerna, Nanson, Jeffrey D., Jia, Xinying, Vajjhala, Parimala R., Hedger, Andrew, Vetter, Irina, Haselhorst, Thomas, Robertson, Avril A. B., Dymock, Brian, Ve, Thomas, Mobli, Mehdi, Stacey, Katryn J. and Kobe, Bostjan (2024). o-Vanillin binds covalently to MAL/TIRAP Lys-210 but independently inhibits TLR2. Journal of Enzyme Inhibition and Medicinal Chemistry, 39 (1) 2313055, 2313055. doi: 10.1080/14756366.2024.2313055 |
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2023 Journal Article Kinetics of severe dengue virus infection and development of gut pathology in micePliego Zamora, Adriana, Kim, Jaehyeon, Vajjhala, Parimala R., Thygesen, Sara J., Watterson, Daniel, Modhiran, Naphak, Bielefeldt-Ohmann, Helle and Stacey, Katryn J. (2023). Kinetics of severe dengue virus infection and development of gut pathology in mice. Journal of Virology, 97 (11) e0125123, e0125123. doi: 10.1128/jvi.01251-23 |
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2023 Conference Publication TLR4 TIR domain higher-order assemblies reveal the structural basis of adaptor recruitment in Toll-like receptor signaling pathwaysNanson, J. D., Muusse, T. W., Li, Y., Pan, M., Landsberg, M. J., Vajjhala, P. R., Ve, T., Stacey, K. J. and Kobe, B. (2023). TLR4 TIR domain higher-order assemblies reveal the structural basis of adaptor recruitment in Toll-like receptor signaling pathways. XXVI IUCr Congress, Melbourne, VIC Australia, 22-29 August 2023. Hoboken, NJ United States: Wiley-Blackwell. doi: 10.1107/s2053273323086631 |
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2023 Conference Publication Structural evolution of TIR-domain signalosomes in mammalian, plant and bacterial immunity characterized by integrative structural biologyKobe, Bostjan, Nanson, Jeffrey D., Manik, Mohammad K., Li, Sulin, Gu, Weixi, Pan, Mengqi, Li, Yan, Muusse, Timothy W., Vajjhala, Parimala R, Stacey, Katryn J., Holmes, Susannah, Darmanin, Connie, Clabbers, Max T. B., Xu, Hongyi, Shi, Yun and Ve, Thomas (2023). Structural evolution of TIR-domain signalosomes in mammalian, plant and bacterial immunity characterized by integrative structural biology. XXVI IUCr Congress, Melbourne, VIC Australia, 22-29 August 2023. Hoboken, NJ United States: Wiley-Blackwell. doi: 10.1107/s2053273323093658 |
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2023 Journal Article TLR4 phosphorylation at tyrosine 672 activates the ERK/c‐FOS signaling module for LPS‐induced cytokine responses in macrophagesCurson, James E. B., Liu, Liping, Luo, Lin, Muusse, Timothy W., Lucas, Richard M., Gunther, Kimberley S., Vajjhala, Parimala R., Abrol, Rishika, Jones, Alun, Kapetanovic, Ronan, Stacey, Katryn J., Stow, Jennifer L. and Sweet, Matthew J. (2023). TLR4 phosphorylation at tyrosine 672 activates the ERK/c‐FOS signaling module for LPS‐induced cytokine responses in macrophages. European Journal of Immunology, 53 (7) e2250056, e2250056. doi: 10.1002/eji.202250056 |
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2022 Journal Article Flow cytometric reporter assays provide robust functional analysis of signaling complexesMuusse, Timothy W., Lee, Morris Y.L., Kim, Hyoyoung, Parat, Marie-Odile, Nanson, Jeffrey D., Kobe, Bostjan, Vajjhala, Parimala R. and Stacey, Katryn J. (2022). Flow cytometric reporter assays provide robust functional analysis of signaling complexes. Journal of Biological Chemistry, 298 (12) 102666, 102666. doi: 10.1016/j.jbc.2022.102666 |
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2021 Journal Article MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallographyClabbers, Max T. B., Holmes, Susannah, Muusse, Timothy W., Vajjhala, Parimala R., Thygesen, Sara J., Malde, Alpeshkumar K., Hunter, Dominic J. B., Croll, Tristan I., Flueckiger, Leonie, Nanson, Jeffrey D., Rahaman, Md. Habibur, Aquila, Andrew, Hunter, Mark S., Liang, Mengning, Yoon, Chun Hong, Zhao, Jingjing, Zatsepin, Nadia A., Abbey, Brian, Sierecki, Emma, Gambin, Yann, Stacey, Katryn J., Darmanin, Connie, Kobe, Bostjan, Xu, Hongyi and Ve, Thomas (2021). MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography. Nature Communications, 12 (1) 2578, 1-14. doi: 10.1038/s41467-021-22590-6 |
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2017 Journal Article Caspase-1 is an apical caspase leading to caspase-3 cleavage in the AIM2 inflammasome response, independent of caspase-8Sagulenko, Vitaliya, Vitak, Nazarii, Vajjhala, Parimala, Vince, James E and Stacey, Katryn J (2017). Caspase-1 is an apical caspase leading to caspase-3 cleavage in the AIM2 inflammasome response, independent of caspase-8. Journal of Molecular Biology, 430 (2), 238-247. doi: 10.1016/j.jmb.2017.10.028 |
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2017 Journal Article Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signalingVe, Thomas, Vajjhala, Parimala, Hedger, Andrew, Croll, Tristan, DiMaio, Frank, Horsefield, Shane, Yu, Xiong, Lavrencic, Peter, Hassan, Zahid, Morgan, Garry P., Mansell, Ashley, Mobli, Mehdi, O'Carroll, Ailis, Chauvin, Brieuc, Gambin, Yann, Sierecki, Emma, Landsberg, Michael J., Stacey, Katryn, Egelman, Edward H. and Kobe, Bostjan (2017). Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling. Nature Structural and Molecular Biology, 24 (9), 743-751. doi: 10.1038/nsmb.3444 |
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2017 Journal Article The molecular mechanisms of signaling by cooperative assembly formation in innate immunity pathwaysVajjhala, Parimala R., Ve, Thomas, Bentham, Adam, Stacey, Katryn J. and Kobe, Bostjan (2017). The molecular mechanisms of signaling by cooperative assembly formation in innate immunity pathways. Molecular Immunology, 86, 23-37. doi: 10.1016/j.molimm.2017.02.012 |
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2016 Journal Article Cryo-EM structure of caspase-8 tandem DED filament reveals assembly and regulation mechanisms of the death-inducing signaling complexFu, Tian-Min, Li, Yang, Lu, Alvin, Li, Zongli, Vajjhala, Parimala R., Cruz, Anthony C., Srivastava, Devendra B., DiMaio, Frank, Penczek, Pawel A., Siegel, Richard M., Stacey, Katryn J., Egelman, Edward H. and Wu, Hao (2016). Cryo-EM structure of caspase-8 tandem DED filament reveals assembly and regulation mechanisms of the death-inducing signaling complex. Molecular Cell, 64 (2), 236-250. doi: 10.1016/j.molcel.2016.09.009 |
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2016 Conference Publication Cytosolic DNA, HIN-200 proteins and inflammasome activationStacey, K. J., Vajjhala, P. R., Sagulenko, V, Thygesen, S., Vitak, N. and Sester, D. P. (2016). Cytosolic DNA, HIN-200 proteins and inflammasome activation. International Congress of Immunology (ICI), Melbourne, VIC Australia, 21-26 August 2016. Weinheim, Germany: Wiley. |
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2016 Conference Publication Molecular arrangement and activation of procaspases-1 and-8 at inflammasomesVajjhala, P., Lu, A., Brown, D., Sagulenko, V, Schroder, K., Stow, J., Wu, H. and Stacey, K. (2016). Molecular arrangement and activation of procaspases-1 and-8 at inflammasomes. ICI 2016 International Congress of Immunology, Melbourne, Australia, 21-26 August 2016. Weinheim, Germany: Wiley. |
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2016 Book Chapter Assessment of inflammasome formation by flow cytometrySester, David P., Zamoshnikova, Alina, Thygesen, Sara J., Vajjhala, Parimala R., Cridland, Simon O., Schroder, Kate and Stacey, Katryn J. (2016). Assessment of inflammasome formation by flow cytometry. Current protocols in immunology. (pp. 14.40.1-14.40.29) edited by Coligan, John E., Bierer, Barbara, Margulies, David H., Shevach, Ethan M. and Strober, Warren. Hoboken, NJ United States: John Wiley & Sons. doi: 10.1002/cpim.13 |
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2015 Journal Article The inflammasome adaptor ASC induces procaspase-8 death effector domain filamentsVajjhala, Parimala R., Lu, Alvin, Brown, Darren L., Pang, Siew Wai, Sagulenko, Vitaliya, Sester, David P., Cridland, Simon O., Hill, Justine M., Schroder, Kate, Stow, Jennifer L., Wu, Hao and Stacey, Katryn J. (2015). The inflammasome adaptor ASC induces procaspase-8 death effector domain filaments. Journal of Biological Chemistry, 290 (49), 29217-29230. doi: 10.1074/jbc.M115.687731 |
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2015 Journal Article A novel flow cytometric method to assess inflammasome formationSester, David P., Thygesen, Sara J., Sagulenko, Vitaliya, Vajjhala, Parimala R., Cridland, Jasmyn A., Vitak, Nazarii, Chen, Kaiwen W., Osborne, Geoffrey W., Schroder, Kate and Katryn J. Stacey (2015). A novel flow cytometric method to assess inflammasome formation. The Journal of Immunology, 194 (1), 455-462. doi: 10.4049/jimmunol.1401110 |
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2014 Journal Article Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assemblyVajjhala, Parimala R., Kaiser, Sebastian, Smith, Sarah J., Ong, Qi-Rui, Soh, Stephanie L., Stacey, Katryn J. and Hill, Justine M. (2014). Identification of multifaceted binding modes for pyrin and ASC pyrin domains gives insights into pyrin inflammasome assembly. Journal of Biological Chemistry, 289 (34), 23504-23519. doi: 10.1074/jbc.M114.553305 |
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2013 Journal Article AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASCSagulenko, V., Thygesen, S. J., Sester, D. P., Idris, A., Cridland, J. A., Vajjhala, P. R., Roberts, T., Schroder, K., Vince, J. E., Hill, J. M., Silke, J. and Stacey, K. J. (2013). AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASC. Cell Death and Differentiation, 20 (9), 1149-1160. doi: 10.1038/cdd.2013.37 |
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2013 Journal Article The structure of the caspase recruitment domain of BinCARD reveals that all three cysteines can be oxidizedChen, Kai-En, Richards, Ayanthi A., Caradoc-Davies, Tom T., Vajjhala, Parimala R., Robin, Gautier, Lua, Linda H. L., Hill, Justine M., Schroder, Kate, Sweet, Matthew J., Kellie, Stuart, Kobe, Bostjan and Martin, Jennifer (2013). The structure of the caspase recruitment domain of BinCARD reveals that all three cysteines can be oxidized. Acta Crystallographica Section D: Biological Crystallography, 69 (5), 774-784. doi: 10.1107/S0907444913001558 |
