Unlocking the proteomics of synaptic glycine receptor complexes (ARC Discovery Projects administered by University of the Sunshine Coast) (2025-2028)
Abstract
This project aims to investigate glycine receptors (GlyRs) that are vital for spinal motor output, pain processing and cortical neuronal migration. This project expects to generate new interdisciplinary knowledge by: i) Using GlyR subtype-specific antibodies and knockout mice in high-fidelity proteomics; ii) Examining the location and functional impacts of GlyR interactors using super-resolution microscopy, shRNA knockdown and artificial synapses. Expected outcomes include the first comprehensive proteomic analysis of synaptic GlyR complexes. This project will also provide significant benefits by characterising a new class of GlyR auxiliary subunits and linking Australian researchers to the international Synaptic Gene Ontologies initiative.
