Directed evolution of multivalency for enhanced selectivity
Abstract
Multivalency is a natural molecular adaptation that enhances the potency and selectivity of biomolecular interactions. While it is common in nature, engineering multivalency in a high-throughput setting remains challenging. To address this, we present MultiKnot, a display platform for the directed evolution of multivalency in constrained peptides. This project aims to establish, refine, and apply MultiKnot to discover novel miticides with species specificity, tailored to curb the varroa mite infestation threatening Australian bees and agriculture. Additionally, we anticipate generating intellectual property with strong commercial potential in the rapidly growing constrained peptide market, while training future biotechnology leaders.
