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2024 Journal Article Sequence diversity in the monooxygenases involved in oxime production in plant defense and signaling: a conservative revision in the nomenclature of the highly complex CYP79 familyKoleva, Donka Teneva, Bengochea, Anthony W., Mellor, Silas B., Ochoa-Fernandez, Rocio, Nelson, David R., Møller, Birger Lindberg, Gillam, Elizabeth M. J. and Sørensen, Mette (2024). Sequence diversity in the monooxygenases involved in oxime production in plant defense and signaling: a conservative revision in the nomenclature of the highly complex CYP79 family. Plant Journal, 120 (3), 1236-1256. doi: 10.1111/tpj.17044 |
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2024 Journal Article Deep mutational scanning of CYP2C19 in human cells reveals a substrate specificity-abundance tradeoffBoyle, Gabriel E., Sitko, Katherine A., Galloway, Jared G., Haddox, Hugh K., Bianchi, Aisha Haley, Dixon, Ajeya, Wheelock, Melinda K., Vandi, Allyssa J., Wang, Ziyu R., Thomson, Raine E. S., Garge, Riddhiman K., Rettie, Allan E., Rubin, Alan F., Geck, Renee C., Gillam, Elizabeth M. J., DeWitt, William S., Matsen, Frederick A. and Fowler, Douglas M. (2024). Deep mutational scanning of CYP2C19 in human cells reveals a substrate specificity-abundance tradeoff. Genetics, 228 (3) iyae156. doi: 10.1093/genetics/iyae156 |
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2024 Journal Article Thermostable fatty acid hydroxylases from ancestral reconstruction of cytochrome P450 family 4 enzymesHarris, Kurt L., Zhang, Yichi, Yang, Jade, Zeigler, Maxwell B., Thomson, Raine E. S., Carrera-Pacheco, Saskya E., Russell, Drake, Okada, Shoko, Strohmaier, Silja J., Gumulya, Yosephine, Scott, Colin, Totah, Rheem A. and Gillam, Elizabeth M. J. (2024). Thermostable fatty acid hydroxylases from ancestral reconstruction of cytochrome P450 family 4 enzymes. Catalysis Science & Technology, 14 (15), 4211-4227. doi: 10.1039/d4cy00090k |
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2024 Journal Article Engineering Biocatalysts for the C−H Activation of Fatty Acids by Ancestral Sequence Reconstruction**Jones, Bethan S., Ross, Connie M., Foley, Gabriel, Pozhydaieva, Nadiia, Sharratt, Joseph W., Kress, Nico, Seibt, Lisa S., Thomson, Raine E. S., Gumulya, Yosephine, Hayes, Martin A., Gillam, Elizabeth M. J. and Flitsch, Sabine L. (2024). Engineering Biocatalysts for the C−H Activation of Fatty Acids by Ancestral Sequence Reconstruction**. Angewandte Chemie, 136 (18). doi: 10.1002/ange.202314869 |
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2024 Journal Article Biocatalysis using thermostable cytochrome P450 enzymes in bacterial membranes - comparison of metabolic pathways with human liver microsomes and recombinant human enzymesJurva, Ulrik, Sandinge, Ann-Sofie, Baek, Jong Min, Avanthay, Mickael, Thomson, Raine E.S., D'Cunha, Stephlina A, Andersson, Shalini, Hayes, Martin A. and Gillam, Elizabeth M. J. (2024). Biocatalysis using thermostable cytochrome P450 enzymes in bacterial membranes - comparison of metabolic pathways with human liver microsomes and recombinant human enzymes. Drug Metabolism and Disposition, 52 (3), 442-251. doi: 10.1124/dmd.123.001569 |
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2024 Journal Article Engineering Biocatalysts for the C‐H Activation of Fatty Acids by Ancestral Sequence ReconstructionJones, Bethan S., Ross, Connie M., Foley, Gabriel, Pozhydaieva, Nadiia, Sharratt, Joseph W., Kress, Nico, Seibt, Lisa S., Thomson, Raine E. S., Gumulya, Yosephine, Hayes, Martin A., Gillam, Elizabeth M. J. and Flitsch, Sabine (2024). Engineering Biocatalysts for the C‐H Activation of Fatty Acids by Ancestral Sequence Reconstruction. Angewandte Chemie International Edition, 63 (18) e202314869, 1-10. doi: 10.1002/anie.202314869 |
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2023 Journal Article Strigolactones and shoot branching: what is the real hormone and how does it work?Dun, Elizabeth A., Brewer, Philip B., Gillam, Elizabeth M. J. and Beveridge, Christine A. (2023). Strigolactones and shoot branching: what is the real hormone and how does it work?. Plant And Cell Physiology, 64 (9), 967-983. doi: 10.1093/pcp/pcad088 |
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2023 Journal Article Solar-powered P450 catalysis: Engineering electron transfer pathways from photosynthesis to P450sAgustinus, Bernadius and Gillam, Elizabeth M.J. (2023). Solar-powered P450 catalysis: Engineering electron transfer pathways from photosynthesis to P450s. Journal of Inorganic Biochemistry, 245 112242, 1-12. doi: 10.1016/j.jinorgbio.2023.112242 |
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2023 Journal Article Opportunities for accelerating drug discovery and development by using engineered drug-metabolizing enzymesGillam, Elizabeth M. J. and Kramlinger, Valerie M (2023). Opportunities for accelerating drug discovery and development by using engineered drug-metabolizing enzymes. Drug Metabolism and Disposition, 51 (3), 392-402. doi: 10.1124/dmd.121.000743 |
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2023 Journal Article Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplastsMellor, Silas B., Behrendorff, James B. Y. H., Ipsen, Johan Ø., Crocoll, Christoph, Laursen, Tomas, Gillam, Elizabeth M. J. and Pribil, Mathias (2023). Exploiting photosynthesis-driven P450 activity to produce indican in tobacco chloroplasts. Frontiers in Plant Science, 13 1049177, 1-17. doi: 10.3389/fpls.2022.1049177 |
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2022 Journal Article Engineering indel and substitution variants of diverse and ancient enzymes using Graphical Representation of Ancestral Sequence Predictions (GRASP)Foley, Gabriel, Mora, Ariane, Ross, Connie M., Bottoms, Scott, Sützl, Leander, Lamprecht, Marnie L., Zaugg, Julian, Essebier, Alexandra, Balderson, Brad, Newell, Rhys, Thomson, Raine E. S., Kobe, Bostjan, Barnard, Ross T., Guddat, Luke, Schenk, Gerhard, Carsten, Jörg, Gumulya, Yosephine, Rost, Burkhard, Haltrich, Dietmar, Sieber, Volker, Gillam, Elizabeth M. J. and Bodén, Mikael (2022). Engineering indel and substitution variants of diverse and ancient enzymes using Graphical Representation of Ancestral Sequence Predictions (GRASP). PL o S Computational Biology, 18 (10) e1010633, e1010633. doi: 10.1371/journal.pcbi.1010633 |
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2022 Journal Article Engineering functional thermostable proteins using ancestral sequence reconstructionThomson, Raine E.S., Carrera-Pacheco, Saskya E. and Gillam, Elizabeth M.J. (2022). Engineering functional thermostable proteins using ancestral sequence reconstruction. Journal of Biological Chemistry, 298 (10) 102435, 1-16. doi: 10.1016/j.jbc.2022.102435 |
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2022 Journal Article Ancestral sequence reconstruction of the CYP711 family reveals functional divergence in strigolactone biosynthetic enzymes associated with gene duplication events in monocot grassesVinde, Marcos H., Cao, Da, Chesterfield, Rebecca J., Yoneyama, Kaori, Gumulya, Yosephine, Thomson, Raine E. S., Matila, Tebogo, Ebert, Birgitta E., Beveridge, Christine A., Vickers, Claudia E. and Gillam, Elizabeth M. J. (2022). Ancestral sequence reconstruction of the CYP711 family reveals functional divergence in strigolactone biosynthetic enzymes associated with gene duplication events in monocot grasses. New Phytologist, 235 (5), 1900-1912. doi: 10.1111/nph.18285 |
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2022 Journal Article Ancestral sequence reconstruction of a cytochrome P450 family involved in chemical defense reveals the functional evolution of a promiscuous, xenobiotic-metabolizing enzyme in vertebratesHarris, Kurt L., Thomson, Raine E.S., Gumulya, Yosephine, Foley, Gabriel, Carrera-Pacheco, Saskya E., Syed, Parnayan, Janosik, Tomasz, Sandinge, Ann-Sofie, Andersson, Shalini, Jurva, Ulrik, Bodén, Mikael and Gillam, Elizabeth M.J. (2022). Ancestral sequence reconstruction of a cytochrome P450 family involved in chemical defense reveals the functional evolution of a promiscuous, xenobiotic-metabolizing enzyme in vertebrates. Molecular Biology and Evolution, 39 (6) msac116. doi: 10.1093/molbev/msac116 |
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2022 Book Chapter Using the evolutionary history of proteins to engineer insertion-deletion mutants from robust, ancestral templates using Graphical Representation of Ancestral Sequence Predictions (GRASP)Ross, Connie M., Foley, Gabriel, Boden, Mikael and Gillam, Elizabeth M. J. (2022). Using the evolutionary history of proteins to engineer insertion-deletion mutants from robust, ancestral templates using Graphical Representation of Ancestral Sequence Predictions (GRASP). Enzyme engineering. (pp. 85-110) edited by Francesca Magnani, Chiara Marabelli and Francesca Paradisi. New York, NY, United States: Humana Press. doi: 10.1007/978-1-0716-1826-4_6 |
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2022 Book Chapter Use of engineered cytochromes P450 for accelerating drug discovery and developmentThomson, Raine E.S., D'Cunha, Stephlina A., Hayes, Martin A. and Gillam, Elizabeth M.J. (2022). Use of engineered cytochromes P450 for accelerating drug discovery and development. Pharmacology and toxicology of cytochrome P450 – 60th anniversary. (pp. 195-252) edited by Hiroshi Yamazaki. Cambridge, MA, United States: Academic Press. doi: 10.1016/bs.apha.2022.06.001 |
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2021 Journal Article Resurrection and characterization of ancestral CYP11A1 enzymesHartz, Philip, Strohmaier, Silja J., EL‐Gayar, Basma M., Abdulmughni, Ammar, Hutter, Michael C., Hannemann, Frank, Gillam, Elizabeth M. J. and Bernhardt, Rita (2021). Resurrection and characterization of ancestral CYP11A1 enzymes. The FEBS Journal, 288 (22) febs.16054, 6510-6527. doi: 10.1111/febs.16054 |
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2020 Book Chapter Directed evolution of enzymesJackson, Colin J., Gillam, Elizabeth M.J. and Ollis, David L. (2020). Directed evolution of enzymes. Comprehensive Natural Products III: Chemistry and biology. (pp. 654-673) London, United Kingdom: Elsevier. doi: 10.1016/B978-008045382-8.00675-4 |
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2020 Journal Article Oxygen surrogate systems for supporting human drug-metabolizing cytochrome P450 enzymesStrohmaier, Silja J., De Voss, James J., Jurva, Ulrik, Andersson, Shalini and Gillam, Elizabeth M. J. (2020). Oxygen surrogate systems for supporting human drug-metabolizing cytochrome P450 enzymes. Drug Metabolism and Disposition , 48 (6), 432-437. doi: 10.1124/dmd.120.090555 |
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2020 Journal Article Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostabilityBart, Aaron G., Harris, Kurt L., Gillam, Elizabeth M. J. and Scott, Emily E. (2020). Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability. Journal of Biological Chemistry, 295 (17), 5640-5653. doi: 10.1074/jbc.ra119.010727 |
