Professor Lars Nielsen
Professor

Lars Nielsen

Email: 
Phone: 
+61 7 334 63986

Background

Professor Lars Nielsen is leading the development of experimental and computational tools to analyse and design complex biological systems. His expertise in metabolic modelling and flux analysis is available nowhere else in Australia – and in few labs across the world. Professor Nielsen’s studies of biological systems as diverse as bacteria, baker's yeast, sugarcane, insects and mammals has attracted industrial partnerships with companies including Dow, Metabolix, Amyris, LanzaTech, Boeing, Virgin Australia and GE. These metabolic engineering partnerships have focussed on developing new ways of producing aviation fuel, various materials and bioactives (antibiotics, biopesticides, monoclonal antibodies). Professor Nielsen is also applying system analysis and design approaches to tissue engineering including novel strategies for generating microtissues for drug screening and using stem cells to produce red and white blood cells for transfusion.

International links

Professor Nielsen collaborates with some of the world’s pre-eminent metabolic engineers. A joint project with Prof Sang Yup Lee (KAIST, Korea) enabled several extended mutual visits to explore use of sugar for higher value products. A separate project focused on producing synthetic aviation fuel based on isoprenoids involves Professor Nielsen collaborating with global synthetic biotechnology company Amyris and leading isoprenoid metabolic engineer Professor Jay Keasling, from UC Berkeley. Professor Nielsen has secured $8million since 2006 from industry through research grants with US, European, Japanese, Korean, New Zealand and Australian companies.

Availability

Professor Lars Nielsen is:
Available for supervision
Media expert

Fields of research

Biological Sciences Industrial biotechnology Microbiology

Qualifications

  • Masters (Coursework), Technical University of Denmark
  • Doctor of Philosophy, The University of Queensland

Research impacts

Research

Systems and synthetic biology. Professor Nielsen is dedicated to both the advancement of bioengineering science and its application to specific problems. Using thermodynamic principles, novel approaches are developed for handling complex, transient dynamics in developing tissue as well as rational design of complex pathways. These novel approaches are used in the design of bioprocesses as diverse as the production of blood cells for transfusion and the production of industrial biopolymers.

Memberships, patents and funding

Professor Nielsen has been granted four patents – two in stem cells and another two in metabolic engineering. He is on the Scientific Advisory Board of InSphero (2009–), a Swiss company commercialising microtissue technology originating in the Nielsen laboratory. He is an editorial board member of ACS Synthetic Biology, Metabolic Engineering, Biotechnology Journal, Biotechnology and Bioengineering and Bioprocess and Biosystems Engineering. Professor Nielsen formed and chaired the 2005 CRC SIIB Industrial Biotechnology Panel to develop an industrial biotechnology strategy for the Australian sugar industry. He successfully lobbied for the inclusion of Industrial Biotechnology in the Queensland Biotechnology Strategic Plan 2005-2015. Since then he has served as expert advisor to government, research bodies, domestic and major international companies such as Dow Chemical, DuPont and GS Caltex. In the past decade, he has been part of successful research grant applications totalling $56 million, with $31.2 million for his own systems and synthetic biology group at AIBN.

Awards and plenaries

Professor Nielsen has received the UQ Foundation Research Excellence Award; and the Australian Institute of Political Science Queensland Young Tall Poppy Award. He was invited to speak at the 2010 Science at the Shine Dome Symposium on Genomics & Mathematics, Canberra; and the Australian Academy of Technical Sciences & Engineering’s National Symposium on Future-Proofing Australia.

Search Professor Lars Nielsen’s works on UQ eSpace

401 works between 1991 and 2025
All (401) Journal Article (291) Book Chapter (15) Conference Publication (91) Other Outputs (4)

221 - 240 of 401 works

2012

Journal Article

METABOLIC PATHWAY ENGINEERING FOR HYALURONIC ACID PRODUCTION

Marcellin, Esteban, Chen, Wendy Y. and Nielsen, Lars K. (2012). METABOLIC PATHWAY ENGINEERING FOR HYALURONIC ACID PRODUCTION. Carbohydrate-Modifying Biocatalysts, 571-584.

METABOLIC PATHWAY ENGINEERING FOR HYALURONIC ACID PRODUCTION

2012

Conference Publication

Major adjustments in metabolic flux occur to accommodate PHB production in sugarcane

McQualter, Richard, Gebbie, Leigh, Dal'Molin, Cristiana G.d., Petrasovits, Lars, Snell, K. D., Nielsen, Lars K. and Brumbley, Stevens (2012). Major adjustments in metabolic flux occur to accommodate PHB production in sugarcane. The 13th International Symposium on Biopolymers (ISBP 2012), Cairns, Australia, 7-10 October.

Major adjustments in metabolic flux occur to accommodate PHB production in sugarcane

2012

Journal Article

Mammalian cells as biopharmaceutical production hosts in the age of omics

Dietmair, Stefanie, Nielsen, Lars K. and Timmins, Nicholas E. (2012). Mammalian cells as biopharmaceutical production hosts in the age of omics. Biotechnology Journal, 7 (1), 75-89. doi: 10.1002/biot.201100369

Mammalian cells as biopharmaceutical production hosts in the age of omics

2012

Conference Publication

Metabolomic Analysis of CHO Cultures with Different Growth Characteristics - Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells

Dietmair, Stefanie, Timmins, Nicholas E., Chrysanthopoulos, Panagiotis, Gray, Peter P., Kromer, Jens O. and Nielsen, Lars K. (2012). Metabolomic Analysis of CHO Cultures with Different Growth Characteristics - Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells. 21st Annual Meeting of the European-Society-for-Animal-Cell-Technology (ESACT), Dublin Ireland, Jun 07-10, 2009. DORDRECHT: SPRINGER. doi: 10.1007/978-94-007-0884-6_6

Metabolomic Analysis of CHO Cultures with Different Growth Characteristics - Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells

2012

Journal Article

Bioreactor for blood product production

Doran, Michael R., Aird, Ian Alexander, Marturana, Flavia, Timmins, Nicholas, Atkinson, Kerry and Nielsen, Lars K. (2012). Bioreactor for blood product production. Cell Transplantation, 21 (6), 1235-1244. doi: 10.3727/096368911X627363

Bioreactor for blood product production

2012

Book Chapter

Metabolic pathway engineering for hyaluronic acid production

Marcellin, Esteban, Chen, Wendy Y. and Nielsen, Lars K. (2012). Metabolic pathway engineering for hyaluronic acid production. Carbohydrate modifying biocatalysts. (pp. 571-584) edited by Peter Grundwald. Singapore: Pan Stanford Publishing. doi: 10.4032/9789814303484

Metabolic pathway engineering for hyaluronic acid production

2012

Conference Publication

Dynamic analysis of Saccharopolyspora erythraea life cycle in bioreactors; a systems approach

Licona Cassani, Cuauthemoc, Marcellin Saldana, Esteban Stefane and Nielsen, Lars K. (2012). Dynamic analysis of Saccharopolyspora erythraea life cycle in bioreactors; a systems approach. Actinobacteria within soil, Munster, Germany, 25-28 October 2012.

Dynamic analysis of Saccharopolyspora erythraea life cycle in bioreactors; a systems approach

2012

Book Chapter

Metabolomic analysis of CHO cultures with different growth characteristics – development of a metabolite extraction protocol for suspension adapted mammalian cells

Dietmair, Stefanie, Timmins, Nicholas E., Chrysanthopoulos, Panagiotis, Gray, Peter P., Krömer, Jens O. and Nielsen, Lars K. (2012). Metabolomic analysis of CHO cultures with different growth characteristics – development of a metabolite extraction protocol for suspension adapted mammalian cells. Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. (pp. 37-41) edited by Nigel Jenkins, Niall Barron and Paula Alves. Dordrecht, Netherlands: Springer.

Metabolomic analysis of CHO cultures with different growth characteristics – development of a metabolite extraction protocol for suspension adapted mammalian cells

2012

Conference Publication

Large Scale Ex Vivo Manufacture of Blood Cells

Timmins, Nicholas E. and Nielsen, Lars K. (2012). Large Scale Ex Vivo Manufacture of Blood Cells. 21st Annual Meeting of the European-Society-for-Animal-Cell-Technology (ESACT), Dublin Ireland, Jun 07-10, 2009. DORDRECHT: SPRINGER. doi: 10.1007/978-94-007-0884-6_92

Large Scale Ex Vivo Manufacture of Blood Cells

2012

Book Chapter

Large scale ex vivo manufacture of blood cells

Timmins, Nicholas E. and Nielsen, Lars K. (2012). Large scale ex vivo manufacture of blood cells. Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009. (pp. 557-571) edited by Nigel Jenkins, Niall Barron and Paula Alves. Dordrecht, Netherlands: Springer.

Large scale ex vivo manufacture of blood cells

2011

Journal Article

AlgaGEM - A genome-scale metabolic reconstruction of Algae based on Chlamydomonas reinhardtii genome

Dal'Molin, Cristiana Gomes de Oliveira, Quek, Lake-Ee, Palfreyman, Robin W. and Nielsen, Lars K. (2011). AlgaGEM - A genome-scale metabolic reconstruction of Algae based on Chlamydomonas reinhardtii genome. BMC Genomics, 12 (S4), s5.1-s5.10. doi: 10.1186/1471-2164-12-S4-S5

AlgaGEM - A genome-scale metabolic reconstruction of Algae based on Chlamydomonas reinhardtii genome

2011

Journal Article

Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture

Arifin, Yalun, Sabri, Suriana, Sugiarto, Haryadi, Krömer, Jens O., Vickers, Claudia E. and Nielsen, Lars K. (2011). Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture. Journal of Biotechnology, 156 (4), 275-278. doi: 10.1016/j.jbiotec.2011.07.003

Deletion of cscR in Escherichia coli W improves growth and poly-3-hydroxybutyrate (PHB) production from sucrose in fed batch culture

2011

Journal Article

Manufactured RBC - Rivers of blood, or an oasis in the desert?

Timmins, N. E. and Nielsen, L. K. (2011). Manufactured RBC - Rivers of blood, or an oasis in the desert?. Biotechnology Advances, 29 (6), 661-666. doi: 10.1016/j.biotechadv.2011.05.002

Manufactured RBC - Rivers of blood, or an oasis in the desert?

2011

Journal Article

Ultra-high-yield manufacture of red blood cells from hematopoietic stem cells

Timmins, Nicholas E., Athanasas, Stavrosia, Gunther, Marko, Buntine, Penelope and Nielsen, Lars K. (2011). Ultra-high-yield manufacture of red blood cells from hematopoietic stem cells. Tissue Engineering Part C-Methods, 17 (11), 1131-1137. doi: 10.1089/ten.tec.2011.0207

Ultra-high-yield manufacture of red blood cells from hematopoietic stem cells

2011

Journal Article

HR index-a simple method for the prediction of oxygen uptake

Wicks, John Richard, Oldridge, Neil B., Nielsen, Lars K. and Vickers, Claudia E. (2011). HR index-a simple method for the prediction of oxygen uptake. Medicine and Science in Sports and Exercise, 43 (10), 2005-2012. doi: 10.1249/MSS.0b013e318217276e

HR index-a simple method for the prediction of oxygen uptake

2011

Journal Article

Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum

Etebari, Kayvan, Palfreyman, Robin W., Schlipalius, David, Nielsen, Lars K., Glatz, Richard V. and Asgari, Sassan (2011). Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum. BMC Genomics, 12 (1) 446, 446.1-446.18. doi: 10.1186/1471-2164-12-446

Deep sequencing-based transcriptome analysis of Plutella xylostella larvae parasitized by Diadegma semiclausum

2011

Journal Article

Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors

Kromer, Jens O., Dietmair, Stefanie, Jacob, Shana S. and Nielsen, Lars K. (2011). Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors. Analytical Biochemistry, 416 (1), 129-131. doi: 10.1016/j.ab.2011.05.019

Quantification of L-alanyl-L-glutamine in mammalian cell culture broth: Evaluation of different detectors

2011

Journal Article

Synthesis of short-chain-length/medium-chain length polyhydroxyalkanoate (PHA) copolymers in peroxisomes of transgenic sugarcane plants

Anderson, David J., Gnanasambandam, Annathurai, Mills, Edwina, O'Shea, Michael G., Nielsen, Lars K. and Brumbley, Stevens M. (2011). Synthesis of short-chain-length/medium-chain length polyhydroxyalkanoate (PHA) copolymers in peroxisomes of transgenic sugarcane plants. Tropical Plant Biology, 4 (3-4), 170-184. doi: 10.1007/s12042-011-9080-7

Synthesis of short-chain-length/medium-chain length polyhydroxyalkanoate (PHA) copolymers in peroxisomes of transgenic sugarcane plants

2011

Journal Article

In vitro production of Helicoverpa baculovirus biopesticides: Automated selection of insect cell clones for manufacturing and systems biology studies

Nguyen, Quan, Qi, Ying Mei, Wu, Yang, Chan, Leslie C. L., Nielsen, Lars K. and Reid, Steven (2011). In vitro production of Helicoverpa baculovirus biopesticides: Automated selection of insect cell clones for manufacturing and systems biology studies. Journal of Virological Methods, 175 (2), 197-205. doi: 10.1016/j.jviromet.2011.05.011

In vitro production of Helicoverpa baculovirus biopesticides: Automated selection of insect cell clones for manufacturing and systems biology studies

2011

Journal Article

Engineering a mammalian super producer

Dietmair, Stefanie, Nielsen, Lars K. and Timmins, Nicholas E. (2011). Engineering a mammalian super producer. Journal of Chemical Technology and Biotechnology, 86 (7), 905-914. doi: 10.1002/jctb.2576

Engineering a mammalian super producer

Current funding

  • 2024 - 2028
    Integrated Design Environment for Advanced Biomanufacturing (IDEA Bio) - Bioplatforms Australia (BPA) NCRIS 2022
    Bioplatforms Australia Ltd
    Open grant
  • 2023 - 2028
    Queensland Metabolomics and Proteomics (Q-MAP) Facility - Bioplatforms Australia (BPA) NCRIS 2022
    Bioplatforms Australia Ltd
    Open grant
  • 2023 - 2028
    Development of reagents for blood product safety
    Australian Red Cross Society
    Open grant
  • 2022 - 2025
    From trash to treasure: engineering waste carbon utilisation in yeast (administered by Macquarie University)
    Macquarie University
    Open grant
  • 2020 - 2027
    ARC Centre of Excellence in Synthetic Biology (ARC Centre of Excellence administered by Macquarie University)
    Macquarie University
    Open grant
  • 2018 - 2025
    Collaboration on Industrial Biotechnology
    Technical University of Denmark
    Open grant

Past funding

  • 2022 - 2024
    Synthetic Biology for Synthesis of Novel Polymers Used in Energy Storage
    Commonwealth Defence Science and Technology Group
    Open grant
  • 2019 - 2024
    BioPlatforms Australia (BPA) Queensland Co-Investment (RICF)
    Bioplatforms Australia Ltd
    Open grant
  • 2017 - 2023
    ARC Training Centre for Biopharmaceutical Innovation
    ARC Industrial Transformation Training Centres
    Open grant
  • 2016 - 2019
    Improving clostridial toxoid production through molecular fermentation maps
    ARC Linkage Projects
    Open grant
  • 2015 - 2018
    Developing an integrated systems and synthetic biology platform to expand the product spectrum of acetogens
    ARC Linkage Projects
    Open grant
  • 2015
    A sensitive, high resolution QTOF mass spectrometer with nanoUPLC system for qualitative and quantitative biomolecule analysis.
    UQ Major Equipment and Infrastructure
    Open grant
  • 2015
    Building a Gas Fermentation Facility for the Production of Fuels and Chemicals
    UQ Major Equipment and Infrastructure
    Open grant
  • 2014 - 2015
    A unique model platform for C4 plant systems and synthetic biology
    United States Asian Office of Aerospace Research and Development
    Open grant
  • 2014 - 2016
    Toward sustainable diesel production using microbial cells: unravelling isoprenoid pathway regulation through systems biology
    ARC Discovery Projects
    Open grant
  • 2013 - 2024
    Bioplatforms Australia Project - Metabolomics (Commonwealth NCRIS 2013 (2013-2023) grant administered by Macquarie University via Bioplatforms Australia Ltd)
    Bioplatforms Australia Ltd
    Open grant
  • 2013 - 2014
    Emerging Biomolecular Platforms and Informatics Project (CRIS grant administered by Macquarie University via Bioplatforms Australian Ltd (BPA))
    Bioplatforms Australia Ltd
    Open grant
  • 2012 - 2016
    Sustainable Dollar Notes and Other Polypropylenes from Bioderived Feedstocks
    ARC Linkage Projects
    Open grant
  • 2012 - 2014
    Transforming greenhouse gases into fuels and chemicals: A systems approach
    UQ Collaboration and Industry Engagement Fund
    Open grant
  • 2012
    Reconstruction of a Genome Scale Model
    LanzaTech New Zealand Limited
    Open grant
  • 2012 - 2014
    Transforming industrial greenhouse gas emissions into useful chemicals: a systems biology approach
    UQ FirstLink Scheme
    Open grant
  • 2012 - 2016
    Clostridial Systems Biology
    Zoetis Australia Research and Manufacturing Pty Ltd
    Open grant
  • 2012
    Plant Fluxomics Platform to understand the interplay of plant metabolic networks
    UWA-UQ Bilateral Research Collaboration Award
    Open grant
  • 2011 - 2019
    Stem Cells Australia (ARC Special Research Initiative administered by the University of Melbourne)
    University of Melbourne
    Open grant
  • 2011 - 2013
    Computational systems biology: understanding mammalian cell fates using genome-scale network models
    ARC Discovery Projects
    Open grant
  • 2011
    Mass spectrometer for biomolecule discovery, structural analysis and quantification.
    UQ Major Equipment and Infrastructure
    Open grant
  • 2011
    Multichannel potentiostats to drive microbial and electrochemical production processes
    UQ Major Equipment and Infrastructure
    Open grant
  • 2010 - 2014
    Metabolomics Australia - Queensland Node
    Queensland Government Smart Futures Co-Investment Fund
    Open grant
  • 2010 - 2011
    Sponsored Research Agreement (Microbes)
    Dow Chemical Australia Limited
    Open grant
  • 2010 - 2015
    Redirecting Carbon Flow through Mesophyll and Bundle Sheath Cells of Sugarcane to Produce Poly-3-Hydroxybutyrate
    ARC Linkage Projects
    Open grant
  • 2010 - 2012
    Sponsored Research Agreement (Plants -Plant Cell Fluxomics)
    Dow Chemical Australia Limited
    Open grant
  • 2010 - 2014
    Queensland Sustainable Aviation Fuel Initiative
    Queensland Government Smart State National and International Research Alliances Program
    Open grant
  • 2010 - 2012
    From Genotype to Phenotype - Systems Biology Bridging the Gap
    ARC Discovery Projects
    Open grant
  • 2010
    Rapid and high-resolution nanocharacterisation of proteins, nanoparticles and polymers
    UQ Major Equipment and Infrastructure
    Open grant
  • 2009 - 2010
    Defining the oxygen and redox conditions for human cord blood stem cell expansion
    Inner Wheel Australia Incorporated
    Open grant
  • 2009 - 2010
    Building a Systems Biology Platform for the Production of a Vaccine to Prevent Enterotoxaemia
    UQ FirstLink Scheme
    Open grant
  • 2009 - 2011
    Production of Neutrophils (ASCC Collaborative Stream 1 - Module 4)
    Australian Stem Cell Centre
    Open grant
  • 2009 - 2010
    Process Modelling Applicable to Biofuels
    Queensland Government Smart Futures Partnerships-Alliances Facilitation Program
    Open grant
  • 2009 - 2011
    Queensland Bio Jet Fuel Initiative
    Queensland Government Smart Futures Partnerships-Alliances Facilitation Program
    Open grant
  • 2009 - 2010
    Evaluation of Fermentation Targets
    Dow Chemical Australia Limited
    Open grant
  • 2009 - 2012
    In-Vitro Production of Baculovirus Biopesticides - A Systems Biology Approach
    ARC Linkage Projects
    Open grant
  • 2008 - 2012
    Korea-Australia Bio-Product Alliance
    Queensland Government Smart State National and International Research Alliances Program
    Open grant
  • 2008 - 2010
    Ex vivo generated allogeneic neutrophils for anti-infective supportive care in acute leukaemia
    Australian Stem Cell Centre
    Open grant
  • 2008 - 2012
    Implementing an Investment Plan for the National Collaborative Research Infrastructure Strategy's Research Capability known as 'Evolving Biomolecular Platforms and Informatics' (administered by BPA)
    Open grant
  • 2008 - 2009
    High Mw Hyaluronic Acid (Wendy Chen)
    CRC for Sugar Industry Innovation through Biotechnology
    Open grant
  • 2007 - 2012
    National Collaborative Research Infrastructure Strategy (NCRIS) - Capability Area 5.1 Evolving Bio-molecular Platforms and Informatics (Sub-capability - Metabolomics)
    National Collaborative Infrastructure Strategy - Queensland Government Contribution
    Open grant
  • 2007 - 2008
    Optimization of hyaluronic acid production by fermentation of the strain streptococcus Equi
    Novozymes A/S
    Open grant
  • 2006 - 2009
    Ex vivo production of neutrophils
    ARC Linkage Projects
    Open grant
  • 2006 - 2010
    The Production of PHB/PHAs in Plants
    CRC for Sugar Industry Innovation through Biotechnology
    Open grant
  • 2004 - 2005
    Neutrophil Pilot Project
    Australian Red Cross Blood Service
    Open grant
  • 2003 - 2006
    EX VIVO PRODUCTION OF MATURE RED BLOOD CELLS FOR TRANSFUSION
    ARC Linkage Projects
    Open grant
  • 2003 - 2006
    Biorefinery
    CRC for Sugar Industry Innovation through Biotechnology
    Open grant
  • 2003 - 2004
    Reduced nitrogen requirements in lactic acid production using Streptococcus bovis
    Toyota Motor Corporation
    Open grant
  • 2002 - 2005
    Metabolic engineering of sugarcane: production of a biodegradable bioplastic as a test-case
    ARC Linkage Projects
    Open grant
  • 2001
    Polyhydroxybutyrate (PHB) Production in Sugarcane
    Bureau of Sugar Experiment Stations
    Open grant
  • 2000
    Determining intracellular metabolic fluxes in animal cell culture.
    ARC Australian Research Council (Small grants)
    Open grant
  • 2000
    To develop the theoretical framework of ME of HA production
    UQ Travel Grants Scheme
    Open grant
  • 2000
    Understanding and Resolving the Problem of Poor Transduction Efficiency Observed in Retroviral Gene Therapy.
    UQ Foundation
    Open grant
  • 1999 - 2001
    Metabolic Engineering of Hyaluronic Acid Production
    ARC Australian Research Council (Large grants)
    Open grant
  • 1999 - 2001
    Self-assembled membranes for protection of foreign tissue transplants
    ARC Australian Research Council (Large grants)
    Open grant
  • 1999
    Understanding and resolving the problem of poor transduction efficiency observed in retroviral gene therapy
    ARC Australian Research Council (Small grants)
    Open grant

Availability

Professor Lars Nielsen is:
Available for supervision

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Supervision history

Current supervision

Completed supervision

Enquiries

Contact Professor Lars Nielsen directly for media enquiries about:

  • Engineering - biological
  • Metabolic engineering
  • Synthetic biology

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